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pInducer(p20) x mKate2-NLS-P2A-HRas(N17)_ires-puro

Plasmid

#167832

Purpose

Inducible expression of dominant negative HRas(N17) and P2A-coupled reporter mKate2-NLS

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

HRAS(N17) (HRAS Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S17N

Promoter

Ubc, TRE

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)

Plasmid

#209086

Purpose

Ngn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expression

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)
BirA

Tags

AviTag

Expression

Mammalian

Mutation

human codon optimized

Promoter

TRE4G and hSyn

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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RAB11A-GFP HDRT Source (pTR 143)

Plasmid

#112012

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-GFP HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Available Since

Aug. 4, 2018

Availability

Academic Institutions and Nonprofits only

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RAB11A-mCherry HDRT Source (pTR 144)

Plasmid

#112013

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-mCherry HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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CD4-C-GFP HDRT Source (pTR 152)

Plasmid

#112018

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-GFP HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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CLTA-GFP HDRT Source (pTR 153)

Plasmid

#112016

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CLTA-GFP HDRT (CLTA Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pHis-TEV-Nsp15

Plasmid

#187656

Purpose

E. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

Depositor

Debra Hansen

Article

Jernigan et al Structure. 2022 Dec 30:S0969-2126(22)004

Insert

Nsp15 (N Severe acute respiratory syndrome coronavirus 2)

Tags

TEV protease cleavable His6-tag

Expression

Bacterial

Mutation

none

Promoter

T7lac

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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CLTA-mCherry HDRT Source (pTR 177)

Plasmid

#112017

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CLTA-mCherry HDRT (CLTA Human)

Use

CRISPR and Synthetic Biology

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTL-DHDDS

Plasmid

#203177

Purpose

Centromeric yeast expression vector for hDHDDS

Depositor

William Sessa

Article

Galosi et al Brain. 2022 Mar 29;145(1):208-223. doi:

Insert

DHDDS (DHDDS Human)

Expression

Yeast

Promoter

RER2

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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BATF-GFP HDRT Source (pTR 146)

Plasmid

#112015

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human BATF gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

BATF-GFP HDRT (BATF Human)

Use

CRISPR and Synthetic Biology

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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