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pTE_19 - pIVT_CMV-synUTR-inactT7-NLS-Cas9-NLS-XTEN-NLS-HbaUTR

Plasmid

#252015

Purpose

In vitro transcription of Cas9 only for mRNA generation

Depositor

Neville Sanjana

Article

Burrell et al bioRxiv 2026.02.19.706875

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Mutation

NA

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-Puro-ArfGAP1_ALPS1-mKate2-3NLS-P2A-eGFP-LMNB1

Plasmid

#250914

Purpose

Dual color expression of mKate2-3xNLS tagged rat ALPS1 and eGFP tagged zebrafish Lamin B1 in mammalian cell lines

Depositor

Philipp Niethammer

Article

Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/

Insert

ArfGAP1-mKate2-3xNLS-P2A-EGFP-LMNB1 (lmnb1, Arfgap1 Rat, Zebrafish)

Tags

EGFP on LMNB1 and mKate2 on ArfGAP1

Expression

Mammalian

Mutation

ARFGAP1 amino acids 196–234 only

Promoter

CMV

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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XZ388 (IVT) CMV-TO-T7-EGFP-SGc(stem-1(3xMS2))cSG-Cre-hybridUTR3

Plasmid

#233424

Purpose

IVT template for LIDAR reporter mRNA; EGFP as marker, Cre recombinase as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

T7-EGFP-SGc(stem-1(3xMS2))cSG-Cre-hybridUTR3

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4-CMV-3xFLAG-NLS/SV40-AcGFP-NES/PKI-P2A-Puro

Plasmid

#228399

Purpose

Encodes 3xFLAG-NLS/SV40-AcGFP-NES/PKI which is a AcGFP with an N-terminal SV40 NLS and a C-terminal PKI NES that shuttles between the nucleus and the cytoplasm

Depositor

Dirk Daelemans

Article

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

3xFLAG-NLS/SV40-AcGFP-NES/PKI

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pBLO44.1_dProtease

Plasmid

#121631

Purpose

CMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

TEV protease

Expression

Mammalian

Promoter

U6 and chicken beta-actin promoter

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CMVd1-NLS-GAL4-iLight2-VP16

Plasmid

#219550

Purpose

NLS-Gal4(1-65)-iLight2-VP16 construc expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

NLS-Gal4(1-65)-iLight2-VP16

Use

AAV

Expression

Mammalian

Mutation

The (SAGG)x4 linker between GAL4(1-65) and the PC…

Promoter

CMVd1

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTS93 pHAGE2 CMVtetO2 GFP-22xGS-MCS

Plasmid

#199346

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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CMVTO-SEAP-26Sfur-B2AD-tevs-KKMP

Plasmid

#179636

Purpose

TEVP-inducible single TM SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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R713-M88-303: CMV51p> Halotag-Hs.HRAS

Plasmid

#159681

Purpose

Mammalian protein expression of HRAS-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Hs.HRAS (HRAS Human)

Tags

Halotag

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6b-V106W

Plasmid

#215825

Purpose

Express CBE6b V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6b V106W-SpCas9-2xUGI (cas9 )

Use

In vitro transcription template

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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AbVec2.1-IGLC2-MscI

Plasmid

#80797

Purpose

Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

Depositor

Hedda Wardemann

Article

Tiller et al J Immunol Methods. 2009 Oct 31;350(1-2):

Type

Empty backbone

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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FLX1.8MitoKaede

Plasmid

#28133

Depositor

Geoffrey Owens

Insert

MitoKaede

Use

Lentiviral

Tags

COX VIII signal sequence

Expression

Mammalian

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-RUNX2

Plasmid

#192926

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of RUNX2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

RUNX2 (RUNX2 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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mito cpVenus control

Plasmid

#186792

Purpose

Identical to mito NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

Depositor

Michael Cohen

Article

Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

Insert

mito cpVenus control

Use

Lentiviral

Expression

Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLENTI_TetOn_Hu_BIRC5(WT)

Plasmid

#136348

Purpose

Lentiviral inducible expression (TetOn) of human BIRC5

Depositor

Guillermo de Cárcer

Insert

BIRC5 (BIRC5 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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Myr.PKCzeta.FLAG

Plasmid

#10802

Depositor

Alex Toker

Article

Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

Insert

PKC zeta (Prkcz Rat)

Tags

FLAG and Myr

Expression

Mammalian

Available Since

July 26, 2006

Availability

Academic Institutions and Nonprofits only
FLAG-TRX

Plasmid

#21615

Depositor

John Kyriakis

Article

Liu et al Mol Cell Biol. 2000 Mar . 20(6):2198-208

Insert

thioredoxin (TXN Human)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pCMV10-VPS13C-W374K I378D L382K-GFP-3xFLAG

Plasmid

#248324

Purpose

Mammalian expression of VPS13C mutant for in vitro studies

Depositor

Karin Reinisch

Article

Li et al Mol Cell. 2026 Jul 7:S1097-2765(26)00417

Insert

VPS13C (VPS13C Human)

Tags

GFP-3xFLAG

Expression

Mammalian

Mutation

W374K, I378D, L382K

Promoter

CMV

Available Since

July 10, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV10-VPS13C-W374K W393D W395K-GFP-3xFLAG

Plasmid

#248325

Purpose

Mammalian expression of VPS13C mutant for in vitro studies

Depositor

Karin Reinisch

Article

Li et al Mol Cell. 2026 Jul 7:S1097-2765(26)00417

Insert

VPS13C (VPS13C Human)

Tags

GFP-3xFLAG

Expression

Mammalian

Mutation

W374K, W393D, W395K

Promoter

CMV

Available Since

July 10, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-IRES2-CRY2-STIM1(238-448)

Plasmid

#248295

Purpose

An expression construct encoding a CRY2-fused STIM1 fragment (a.a.238-448) linked to EGFP through an IRES2 element.

Depositor

Sangkyu Lee

Article

Kook et al Mol Brain. 2023 Oct 17;16(1):73. doi: 10

Insert

EGFP and CRY2-fused STIM1 fragment (a.a.238-448) (STIM1 )

Tags

EGFP

Mutation

deleted amino acids 1-237,449-685

Promoter

CMV

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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