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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 66,745 results for: nin

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Showing: 10061 - 10080 of 66745 results

  3. pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE

    Plasmid
    #138144
    Purpose
    Expresses PguCas13b in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac
    Depositor
    Neville Sanjana
    Publication
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    PguCas13b
    Use
    Lentiviral
    Tags
    NES
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138144-plasmid-map-sequence-id-272529

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  7. pKK-MBP-TEV

    Plasmid
    #105771
    Purpose
    Expression of your protein of interest in fusion with MBP at the N-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    MBP-TEV
    Expression
    Mammalian
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105771-plasmid-map-sequence-id-203360

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  15. pDualEX-SMN2

    Plasmid
    #214431
    Purpose
    Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the exclusion of the frame-shifting cassette exon 7
    Depositor
    Chaolin Zhang
    Publication
    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SMN2 EXON 7 (SMN2 Human)
    Expression
    Mammalian
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    214431-plasmid-map-sequence-id-424357

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  18. pEVOL-NBK-1

    Plasmid
    #207639
    Purpose
    A plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.
    Depositor
    Cole DeForest
    Publication
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    Pyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
    Expression
    Bacterial
    Mutation
    The evolved synthetase NBK-1 contains mutations Y…
    Promoter
    araBAD
    Available since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207639-plasmid-map-sequence-id-409890

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  20. pJAT74

    Plasmid
    #204296
    Purpose
    pJAT plasmid with multiple cloning site for introducing custom payload. One U6 promoter.
    Depositor
    David Stern
    Publication
    Stern et al bioRxiv 2023.06.17.545412
    Type
    Empty backbone
    Use
    CRISPR
    Available since
    Aug. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    204296-plasmid-map-sequence-id-406186

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Showing: 10061 - 10080 of 66745 results