173,384 results

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pDESTsplice

Plasmid

#32484

Depositor

Stefan Stamm

Article

Kishore et al Gene. 2008 Dec 31;427(1-2):104-10. Epub

Type

Empty backbone

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pSF280

Plasmid

#167675

Purpose

Intermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCFD1-dU6:1gRNA

Plasmid

#49408

Purpose

expression of gRNA under control of the Drosophila U6:1 promoter

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

dU6-1:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-1

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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TRUPATH Triple Gaq

Plasmid

#196058

Purpose

Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

Depositor

Justin English

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Inserts

GAlphaQ-RLuc8 (GNAQ Human)
GGamma9-GFP2
GBeta3 (GNB3 Human)

Use

Luciferase

Tags

GFP2 and GSAG linker

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Alpha-Synuclein-His

Plasmid

#186802

Purpose

Codon-optimized bacterial expression plasmid. Expresses Alpha-Synuclein-His.

Depositor

Lalit Deshmukh

Article

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

Alpha-Synuclein-His (SNCA Human)

Tags

His6

Expression

Bacterial

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-RiboL1-jGCaMP8s

Plasmid

#169247

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRU45

Plasmid

#167667

Purpose

ENTRY vector for gRNA5 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pYHY1

Plasmid

#215926

Purpose

E. coli-Bacteroides shuttle vector.

Depositor

Shannon Sirk

Article

Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

Type

Empty backbone

Use

Synthetic Biology; E. coli-bacteroides shuttle ve…

Expression

Bacterial

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor MCS Rosa26

Plasmid

#37200

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

Inserts

Rosa26 left targeting arm
MCS
Rosa26 right targeting arm

Use

Genomic targeting - zinc finger

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP‐RNASEH2A

Plasmid

#108700

Purpose

For expression of N-terminally eGFP-tagged human RNASEH2A in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH2A (RNASEH2A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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