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pRU46

Plasmid

#167668

Purpose

ENTRY vector for gRNA6 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5_CKAR3

Plasmid

#238411

Purpose

Mammalian expression of CKAR3 under a CMV promotor.

Depositor

Haining Zhong

Article

Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

Insert

CKAR3 (PKC sensor)

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CEPIA3mt

Plasmid

#58219

Purpose

Green fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

CEPIA3mt

Tags

myc

Expression

Mammalian

Mutation

GCaMP2 N263Y E282V E334D M339L

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pTET2-RlucCP-3MS2

Plasmid

#198354

Purpose

Part of Renilla reporter system, encodes Renilla luciferase with MS2 stem loops in the 3'UTR

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

Renilla Luciferase

Use

Luciferase

Expression

Mammalian

Promoter

pTET2

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pfMDH_WT

Plasmid

#67721

Purpose

Expresses WT mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

Depositor

Justin Siegel

Article

Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

Insert

Mannitol 2-Dehydrogenase from Pseudomonas fluorescens

Tags

6x-His tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pJMP3653

Plasmid

#222348

Purpose

Expression vector with PabstBR promoter, kanR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Type

Empty backbone

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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M13cp-dg3 hp

Plasmid

#218095

Purpose

Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pIII

Depositor

Kevin Esvelt

Article

Gretton et al bioRxiv 2024.03.20.585782

Type

Empty backbone

Expression

Bacterial

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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SepRS(2)/pSertRNA(B4)/EF-Sep

Plasmid

#173897

Purpose

The plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.

Depositor

Jason W Chin

Article

Rogerson et al Nat Chem Biol. 2015 Jul;11(7):496-503. d

Inserts

SepRS(2)
pSertRNA
EF-Sep

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hM3D(Gq)-mCherry

Plasmid

#50476

Purpose

Gq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoter

Depositor

Bryan Roth

Has Service

AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

hM3D(Gq)-mCherry (CHRM3 Human)

Use

AAV

Tags

mCherry

Mutation

See supplemental documents for DREADD mutations

Promoter

CaMKIIa

Available Since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

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pICH41800

Plasmid

#48020

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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