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SpCas9 TadCBEa

Plasmid

#193782

Purpose

Express TadCBEa (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDa-SpCas9D10A

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Circular-oc-CVB3-GFP

Plasmid

#226262

Purpose

Production of in vitro circularizable RNA via IVT

Depositor

Prashant Mali

Article

Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

Insert

EGFP

Use

In vitro transcription

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-ATG5

Plasmid

#99573

Purpose

Expresses Cas9 with sgRNA targeting Exon 7 of ATG5

Depositor

Edward Campbell

Article

Imam et al J Virol. 2016 Jan 13. pii: JVI.03033-15.

Insert

gRNA targeting ATG5 (ATG5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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CMV500 A-CREB

Plasmid

#33371

Depositor

Charles Vinson

Article

Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

Insert

CREB (Creb1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Dominant Negative (see notes)

Promoter

CMV

Available Since

Dec. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pSFFV_sfCherry3V(1-10)

Plasmid

#117483

Purpose

Lentiviral vector for constitutive expression of sfCherry3V(1-10)

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

sfCherry3V(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

6 substitutions (K45R, G52D, L83W, T106A, K182R, …

Promoter

SFFV

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pWN_U6-TRAC-miniGag-Cas9

Plasmid

#228959

Purpose

miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag-Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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1099 pcDNA GFP FKHR AAA

Plasmid

#9023

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

FKHR AAA (FOXO1 Human)

Tags

GFP

Expression

Mammalian

Mutation

T24A S256A S319A

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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LRG2.1_Neo

Plasmid

#125593

Purpose

Lentiviral expression of sgRNA with GFP and neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-c-Met

Plasmid

#31786

Depositor

Xin Chen

Article

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

Met (MET Human)

Use

Entry vector

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pWN_U6-B2M-miniGag-Cas9

Plasmid

#228958

Purpose

miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag-Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pYES2-gRNA-hyg-MCS

Plasmid

#107734

Purpose

E. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.

Depositor

Sheng Yang

Article

Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

Insert

X-3 site gRNA expression cassettes

Use

CRISPR

Expression

Yeast

Promoter

SNR52p

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-EF1a-i53

Plasmid

#216243

Purpose

Express i53 in mammalian cells as a HDR enhancer

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

i53

Tags

Flag

Expression

Mammalian

Promoter

EF1a

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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CuO-LacI-NLS–SNAPf

Plasmid

#227368

Purpose

Lentiviral expression of LacI–SNAP

Depositor

David Pellman

Article

Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

Insert

LacI NLS SNAPf

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-44B12mut23(Ser5ph)-SNAP

Plasmid

#174877

Purpose

PiggyBac transposon vector for expression of RNAPII Ser5ph mintbody

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

RNAPII Ser5ph mintbody-SNAP

Use

Piggybac vector

Expression

Mammalian

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGZ108 (pFA6a-3FLAG-MNase-kanMX6)

Plasmid

#70231

Purpose

ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope

Depositor

Steven Henikoff

Article

Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

Insert

3xFLAG tag and aa 83-231 of Mnase

Use

Yeast genomic targeting

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-dInPAkt

Plasmid

#181834

Purpose

Genetically encoded single-color sensor for monitoring plasma membrane PI(3,4,5)P2 dynamics in living cells. Based on dimerization-dependent red fluorescent protein (ddRFP).

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-dInPAkt

Tags

N-terminal targeting sequence from Lyn kinase, dd…

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pTnsABC

Plasmid

#160031

Purpose

Expresses V. cholerae TnsABC

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTnsA, VchTnsB, VchTnsC

Use

CRISPR; Transposon

Expression

Bacterial

Mutation

no

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP VAMP2

Plasmid

#42308

Depositor

Thierry Galli

Article

Martinez-Arca et al Proc Natl Acad Sci U S A. 2003 Jul 22;10

Insert

VAMP 2

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2013

Availability

Academic Institutions and Nonprofits only

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pSFFV-SpyTag-sfCherry2(11)-TagBFP

Plasmid

#117485

Purpose

Expresses tandem tag (SpyTag002-GG-sfCherry2(11) fused to N-terminal of TagBFP) in mammalian cells

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

SpyTag002-GG-sfCherry2(11)

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Promoter

SFFV

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pETDuet1_His-TEV-EGFP

Plasmid

#223723

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

EGFP, not monomeric (has A207)

Expression

Bacterial

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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