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pDEST-FRT/TO-eGFP-REV7

Plasmid

#114128

Purpose

Expresses N-terminally tagged eGFP-REV7 in mammalian cells

Depositor

Daniel Durocher

Publication

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

REV7 (MAD2L2 Human)

Use

Genomic integration, tetracycline inducible

Tags

eGFP

Expression

Mammalian

Promoter

CMV/TetO2

Available since

Aug. 15, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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LiOn-CAG∞GFP

Plasmid

#154015

Purpose

Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein EGFP from a CAG promoter upon action of the piggyBac transposase

Depositor

Jean Livet

Publication

Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

Insert

EGFP

Expression

Mammalian

Promoter

CAG

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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LiOn-CAG∞RFP

Plasmid

#154016

Purpose

Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase

Depositor

Jean Livet

Publication

Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

Insert

mRFP1

Expression

Mammalian

Mutation

H210P

Promoter

CAG

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR4_TAF15

Plasmid

#26368

Depositor

Thomas Tuschl

Publication

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Insert

TAF15 (TAF15 Human)

Use

Gateway Cloning

Available since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pAG304GAL-ccdB

Plasmid

#14135

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Puro

Plasmid

#170317

Purpose

Expresses HA and EGFP tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Publication

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGWB551

Plasmid

#74881

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pT2-shP53

Plasmid

#124261

Purpose

Expresses shRNA targeting P53. Construct has inverted repeats to be used in Sleeping beauty system.

Depositor

Maria Castro

Publication

Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

Insert

shP53

Expression

Mammalian

Available since

April 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

9 Articles

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pRK5-HA-SAMTOR

Plasmid

#100513

Purpose

Expresses HA tagged SAMTOR in mammalian cells

Depositor

David Sabatini

Publication

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SAMTOR (BMT2 Human)

Tags

HA

Expression

Mammalian

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCB59 pDEST crybb1:mKate2

Plasmid

#195986

Purpose

Transgenesis backbone

Depositor

Markus Affolter, Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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negMaLionB

Plasmid

#113911

Purpose

negative control for Blue ATP sensor

Depositor

Tetsuya Kitaguchi

Publication

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

negMaLionB

Expression

Mammalian

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-PIWI

Plasmid

#11591

Depositor

Edward Chan

Publication

Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ago2 (AGO2 Human)

Tags

GFP

Expression

Mammalian

Mutation

contain only aa482-869

Available since

June 30, 2006

Availability

Academic Institutions and Nonprofits only
pRS426 Gal TDP43

Plasmid

#27466

Depositor

Aaron Gitler

Publication

Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

Insert

TDP-43 (TARDBP Human)

Expression

Yeast

Available since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only
pWN_U6-B2M-miniGag

Plasmid

#228957

Purpose

miniEDV production plasmid. Expresses the miniGag (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Publication

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

miniGag

Expression

Mammalian

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti_EF1a_Flag_TP53_R248W

Plasmid

#183114

Purpose

Expresses flag-tagged p53 with R248W mutation in mammalian cells

Depositor

William Kaelin

Publication

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed arginine 248 to tryptophan

Promoter

EF1a

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-RET

Plasmid

#116787

Purpose

Lentiviral expression of RET

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RET (RET Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHAGE-SRSF2

Plasmid

#116793

Purpose

Lentiviral expression of SRSF2

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SRSF2 (SRSF2 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-AKT1

Plasmid

#116710

Purpose

Lentiviral expression of AKT1

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

AKT1 (AKT1 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR-BLINK2

Plasmid

#117075

Purpose

BLINK2 gene in pDONR vector for Gateway cloning system

Depositor

Anna Moroni

Publication

Alberio et al Nat Methods. 2018 Nov;15(11):969-976. do

Insert

BLINK2

Use

Gateway cloning system

Mutation

glutamine 112 changed into aspartate (original LO…

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSR49

Plasmid

#69259

Purpose

phlh-8::tagBFP2

Depositor

Sander van den Heuvel

Publication

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

TagBFP2

Use

Cre/Lox

Expression

Worm

Mutation

codon-optimzed index 1.0, TagBFP2 contains 2 sing…

Promoter

hlh-8

Available since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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