We narrowed to 11,847 results for: NSI

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pEBTet-SNAP-HCA_sensor

Plasmid

#127182

Purpose

Eukaryotic expression of the fusion protein used for sulfa-drugs sensing

Depositor

Kai Johnsson

Article

Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

Insert

SNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA

Tags

HisTag and StrepTag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-BsmBI_IRES_BSD

Plasmid

#162804

Purpose

STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI_IRES_Blasticidin

Use

Piggybac

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHES609 [prNOp7-PBAC]

Plasmid

#103017

Purpose

A S. cerevisiae plasmid. A photosensitive adenylate cyclase that produces cAMP in response to blue light. Used to activate PKA.

Depositor

Hana El-Samad

Article

Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

Insert

bPAC

Expression

Yeast

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAL189

Plasmid

#22278

Purpose

Expresses Intersectin DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

IntersectinDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)

Use

Synthetic Biology

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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hP14Gal4

Plasmid

#84364

Purpose

Gal4-PRDM14 funsion plasmid for luciferase recruitment assay

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

PRDM14 (PRDM14 Human)

Tags

Gal4 and HA tag

Expression

Mammalian

Mutation

silent mutations at coding silent 1068CtoT, 1629T…

Promoter

CMV/TO

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pMZ1001

Plasmid

#204398

Purpose

A pC-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.

Depositor

Lauren Andrews

Article

Zeng et al ACS Synth Biol. 2023 Dec 11. doi: 10.102

Inserts

quorum sensing regulator RpaR (rpaR )
GFP

Use

Synthetic Biology

Promoter

PftsH and Synthetic promoter PRpa0002

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pXCX.CMV.CREB.WPRE

Plasmid

#38050

Depositor

James Uney

Article

Glover et al Neuroreport. 2004 May 19;15(7):1171-5.

Insert

CREB (Creb1 Rat)

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-CAHS1

Plasmid

#90030

Purpose

To express GFP-tagged CAHS1 in mammalian cells transiently

Depositor

Takekazu Kunieda

Article

Yamaguchi et al PLoS One. 2012;7(8):e44209.

Insert

CAHS1

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_TOP1

Plasmid

#68423

Purpose

Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP1 construct

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pMTG02-Amp-FRT

Plasmid

#128271

Purpose

Plasmid that produces the following fusion protein: TetR-LOV-RRRG(Degron)

Depositor

Gabor Balazsi

Article

Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

Insert

TetR-LOV-RRRG

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCol-TGM-luc.1309

Plasmid

#32720

Depositor

Scott Lowe

Article

Premsrirut et al Cell. 2011 Apr 1;145(1):145-58.

Insert

luciferase

Use

Mouse Targeting and RNAi

Promoter

TRE and Tre

Available Since

March 2, 2012

Availability

Academic Institutions and Nonprofits only
pPRIME-TET-GIN-recipient

Plasmid

#11656

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP-IRES-Neo expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

GFP, IRES, and Neo

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pTorPE-G-GECO1.1

Plasmid

#32467

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.1

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pCAGGS_2pabPAC

Plasmid

#158968

Purpose

2P-activatable bPAC for transient expression

Depositor

Michiyuki Matsuda

Article

Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

Insert

2pabPAC

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSB1K3_pBen-sfGFP_J23101-BenR

Plasmid

#128125

Purpose

Expressing sfGFP under control of benzoate responsive promoter (pBen) and expressing BenR transcription factor gene under constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

sfGFP and BenR

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBen and J23101

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pACBB-TetT-LVA

Plasmid

#32555

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

TetR

Use

Synthetic Biology

Tags

LVA tag

Expression

Bacterial

Promoter

Consitutive lac

Available Since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pBRC169

Plasmid

#79670

Purpose

DsREDmonomer-based BiFC vector for transient expression of RC (169-225) in plants.

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Type

Empty backbone

Use

Reporter plasmid

Tags

DsRED-monomer (169–225)/RC169

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pLBC0010

Plasmid

#172774

Purpose

Level 0 module containing NT1_CTPSSU

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

Gene sequence for Rubisco small subunit (SSU) chloroplast transit peptide (CTP) of Arabidopsis thaliana

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0011

Plasmid

#172775

Purpose

Level 0 module containing NT2_CTPSSU

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

Gene sequence for Rubisco small subunit (SSU) chloroplast transit peptide (CTP) of Arabidopsis thaliana

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAcBac1-3nitroY-A7-RS

Plasmid

#141173

Purpose

Expression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acids

Depositor

Ryan Mehl

Article

Porter et al ACS Chem Biol. 2019 Jun 21;14(6):1328-13

Insert

A7 3nY tRNA synthetase

Use

Lentiviral

Expression

Mammalian

Mutation

P186T L274R N311G C313N

Promoter

CMV

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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