We narrowed to 18,567 results for: puro

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pSpCas9_human_ADAR2_ccC

Plasmid

#158112

Purpose

deletion hADAR2

Depositor

Emanuela Felley-Bosco

Publication

Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

Insert

ADAR2 (ADARB1 Human)

Expression

Mammalian

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9_human_ADAR2_ccA

Plasmid

#158114

Purpose

deletion hADAR2

Depositor

Emanuela Felley-Bosco

Publication

Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

Insert

ADAR2 (ADARB1 Human)

Expression

Mammalian

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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HiFi dCas9 KRAB

Plasmid

#188500

Purpose

Expresses FLAG tagged HiFi dCas9 KRAB

Depositor

Isaac Hilton

Publication

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A/R691A/H840A

Promoter

EF1a

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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doraWT_rescue_construct

Plasmid

#190609

Purpose

Puromycin-selectable expression of Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Publication

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Expression

Insect

Promoter

Actin-5c

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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dCas12j3 KRAB

Plasmid

#188507

Purpose

Expresses FLAG tagged dCas12j3 KRAB

Depositor

Isaac Hilton

Publication

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j3

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D413A

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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dCas12j2 VPR

Plasmid

#188518

Purpose

Expresses FLAG tagged dCas12j2 VPR

Depositor

Isaac Hilton

Publication

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j2

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D394A

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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SpRY dCas9 VPR

Plasmid

#188511

Purpose

Expresses FLAG tagged SpRY dCas9 VPR

Depositor

Isaac Hilton

Publication

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSBbi-NLSmCherryNLS Clover-HDHB

Plasmid

#190223

Purpose

Fluorescent cell cycle reporter

Depositor

Laura Heiser

Publication

Gross et al Nat Commun. 2023 Jun 10;14(1):3450. doi:

Inserts

Human DNA Helicase B (amino acids 994-1087) (HELB Human)
NLS-mCherry-NLS-P2A-Puromycin

Use

Transposon

Tags

Clover fluorescent protein

Expression

Mammalian

Mutation

Amino Acids 994-1087

Promoter

EF1a and RPBSA

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-Lenti-CBh.6-SOD1

Plasmid

#188460

Purpose

For knockdown-replacement of SOD1 gene (SOD1 KDR lentiviral transfer plasmid, Fig 4)

Depositor

Erik Dent

Publication

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

SOD1 (SOD1 Human)

Use

Lentiviral

Promoter

CBh

Available since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_mCherry_NT135

Plasmid

#183121

Purpose

pLentiGuide modified to express mCherry and a non-targeting sgRNA

Depositor

William Kaelin

Publication

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

NT135

Use

Lentiviral

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_mCherry_sgEPAS1#1

Plasmid

#183122

Purpose

pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

Depositor

William Kaelin

Publication

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgEPAS1-1

Use

Lentiviral

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_sg83-PDGFR_EF1a_iRFP670

Plasmid

#186311

Purpose

Plasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Publication

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg83-PDGFR
iRFP670

Tags

His, myc

Expression

Mammalian

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_sg88-PDGFR_EF1a_eBFP2

Plasmid

#186312

Purpose

Plasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Publication

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg88-PDGFR
eBFP2

Tags

His, myc

Expression

Mammalian

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_P9-PDGFR_EF1a_mPlum

Plasmid

#186307

Purpose

Plasmid for inducible mammalian cell expression of the P9 helixCAM and constitutive expression of an identifying fluorescent protein (mPlum). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Publication

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

P9-PDGFR
mPlum

Tags

HA, myc

Expression

Mammalian

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_AP10-PDGFR_EF1a_mOrange

Plasmid

#186308

Purpose

Plasmid for inducible mammalian cell expression of the AP10 helixCAM and constitutive expression of an identifying fluorescent protein (mOrange). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Publication

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

AP10-PDGFR
mOrange

Tags

HA, myc

Expression

Mammalian

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_P3-PDGFR_EF1a_iRFP670

Plasmid

#186305

Purpose

Plasmid for inducible mammalian cell expression of the P3 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Publication

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

P3-PDGFR
iRFP670

Tags

HA, myc

Expression

Mammalian

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19_Ntag_BbsI-Mammalian

Plasmid

#186674

Purpose

N-terminal tag cassette with BbsI restriction sites for CRISPR donor plasmid.

Depositor

Astrid Haase

Publication

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Mammalian N-terminal Tag

Tags

3xFlag-3xHA

Expression

Mammalian

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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PUC19_C_tag

Plasmid

#186659

Purpose

Empty donor plasmid for C-terminal tag of 3xFlag-3xHA.

Depositor

Astrid Haase

Publication

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

C-terminal 3xFlag-3xHA Tag

Tags

3xFlag-3xHA

Expression

Insect

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-mitoX4-mCerulean

Plasmid

#185807

Purpose

Mitochondrial targetted (COX8) mCerulean

Depositor

Dipayan Chaudhuri

Publication

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

mito-mCerulean

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-mitoX4-mVenus

Plasmid

#185808

Purpose

Mitochondrial targetted (COX8) mVenus

Depositor

Dipayan Chaudhuri

Publication

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

mito-mVenus

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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