Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 2,122 results for: RP1

Showing: 2001 - 2040 of 2122 results

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MSCV-HA-Nr4a1-AA

Plasmid

#160942

Purpose

Generation of retrovirus for the overexpression of Nr4a1 mutant (287C 288EAA)

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 (Nr4a1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Change Cys 287 Glu 288 into Ala Ala

Promoter

MSCV-LTR

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC16A10_STOP

Plasmid

#161112

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC16A10 (SLC16A10 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC26A8_STOP

Plasmid

#161443

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC26A8 (SLC26A8 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223_OMA1_p.P177S

Plasmid

#81441

Purpose

Gateway Donor vector containing OMA1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

OMA1 (OMA1 Human)

Use

Gateway entry vector

Mutation

P177S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBKWH-Lam

Plasmid

#133899

Purpose

Negative control. Expression of Gal4BD-Lam hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

LMNA (Lam) (LMNA Human)

Expression

Yeast

Promoter

T7

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC25A34_STOP

Plasmid

#161171

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A34 (SLC25A34 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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FGGY gRNA (BRDN0001145631)

Plasmid

#77109

Purpose

3rd generation lentiviral gRNA plasmid targeting human FGGY

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FGGY (FGGY Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ATR gRNA (BRDN0001144763)

Plasmid

#77549

Purpose

3rd generation lentiviral gRNA plasmid targeting human ATR

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ATR (ATR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ATR gRNA (BRDN0001147587)

Plasmid

#77551

Purpose

3rd generation lentiviral gRNA plasmid targeting human ATR

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ATR (ATR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ATR gRNA (BRDN0001145195)

Plasmid

#77550

Purpose

3rd generation lentiviral gRNA plasmid targeting human ATR

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ATR (ATR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ERECTA C6_pECIA2

Plasmid

#115127

Purpose

Bait vector ERECTA C6_pECIA2 should be used with prey vector ERECTA C6_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G26330 (ER Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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ERECTA C6_pECIA14

Plasmid

#114927

Purpose

Prey vector ERECTA C6_pECIA14 should be used with bait vector ERECTA C6_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G26330 (ER Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_C1D (pP18(T)_B9-AVA4070)

Plasmid

#239296

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting C1D

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting C1D (C1D Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-DIO-cOpn5-T2A-EGFP

Plasmid

#237858

Purpose

The transfer plasmid for packaging AAV expressing Cre-dependent chicken OPN5 was constructed by inserting the OPN5 coding sequence into the multiple cloning site under the control of EF1α promoter

Depositor

Minmin Luo

Article

Dai et al Sci China Life Sci. 2022 Jul;65(7):1271-

Insert

Opn5 (OPN5 Chicken)

Use

AAV and Cre/Lox

Promoter

EF1α

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-DIO-cOpn5-T2A-mcherry

Plasmid

#238009

Purpose

The transfer plasmid for packaging AAV expressing Cre-dependent chicken OPN5 was constructed by inserting the OPN5 coding sequence into the multiple cloning site under the control of EF1α promoter

Depositor

Minmin Luo

Article

Dai et al Sci China Life Sci. 2022 Jul;65(7):1271-

Insert

Opn5 (OPN5 Chicken)

Use

AAV and Cre/Lox

Promoter

EF1α

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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DHFR-C-HiBiT

Plasmid

#207119

Purpose

Bacterially expressed DHFR-HiBiT for Ni-NTA purification

Depositor

James Inglese

Article

Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

Insert

Human-dihydrofolate reductase-HiBiT-10xHis (DHFR Human)

Tags

10xHis-tag and HiBiT tag

Expression

Bacterial

Mutation

Codon optmization for expression in BL21DE3

Promoter

T7

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pRP.ABCA4 c.5461-10T

Plasmid

#226553

Purpose

Minigene encoding the ABCA4 c.5461-10T mutation

Depositor

Dave Wee

Article

Tabaglio et al Mol Ther Nucleic Acids. 2025 Jan 7;36(1)

Insert

ABCA4 (ABCA4 Human)

Expression

Mammalian

Mutation

c.5461-10T intronic mutation

Promoter

CAG

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_VAV2_COL27A1

Plasmid

#205952

Purpose

Express mEGFP-tagged fusion protein, VAV2_COL27A1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

VAV2_COL27A1 (VAV2, COL27A1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_ZC3HAV1_ABL2

Plasmid

#205957

Purpose

Express mEGFP-tagged fusion protein, ZC3HAV1_ABL2 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ZC3HAV1_ABL2 (ZC3HAV1, ABL2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_S100A9_KRT4

Plasmid

#205916

Purpose

Express mEGFP-tagged fusion protein, S100A9_KRT4 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

S100A9_KRT4 (S100A9, KRT4 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SLAMF9-COMP5AP-AviTag-9xHis

Plasmid

#157212

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SLAMF9 (SLAMF9 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SLAMF8-COMP5AP-AviTag-9xHis

Plasmid

#157195

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SLAMF8 (SLAMF8 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MDM4_Deletion_Downstream_gRNA_2

Plasmid

#195137

Purpose

gRNA in a third generation gRNA backbone with mCherry, targeting region immediately downstream of MDM4, to be used with MDM4_Deletion_Upstream_gRNA_1/2 for MDM4 deletion

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 Deletion Downstream gRNA 2 (MDM4 Human)

Expression

Mammalian

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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MDM4_CRISPRi_gRNA_2

Plasmid

#195133

Purpose

gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 CRISPRi gRNA 2 (MDM4 Human)

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MDM4_CRISPRi_gRNA_1

Plasmid

#195132

Purpose

gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 CRISPRi gRNA 1 (MDM4 Human)

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MDM4_Deletion_Downstream_gRNA_1

Plasmid

#195136

Purpose

gRNA in a third generation gRNA backbone with mCherry, targeting region immediately downstream of MDM4, to be used with MDM4_Deletion_Upstream_gRNA_1/2 for MDM4 deletion

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 Deletion Downstream gRNA 1 (MDM4 Human)

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-HA-Nr4a1-Zf2

Plasmid

#160944

Purpose

Generation of retrovirus for the overexpression of Nr4a1 mutant (zinc finger 2 deletion)

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 (Nr4a1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Deletion of Zinc finger 2 (C306 to C330)

Promoter

MSCV-LTR

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-HA-Nr4a1-Zf1

Plasmid

#160943

Purpose

Generation of retrovirus for the overexpression of Nr4a1 mutant (zinc finger 1 deletion)

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 (Nr4a1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Deletion of Zinc finger 1 (C270 to C290)

Promoter

MSCV-LTR

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
DGKH gRNA (BRDN0001144863)

Plasmid

#78043

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKH

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKH (DGKH Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
DGKH gRNA (BRDN0001487100)

Plasmid

#78044

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKH

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKH (DGKH Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
DGKH gRNA (BRDN0001149163)

Plasmid

#78045

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKH

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKH (DGKH Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
AK8 gRNA (BRDN0001148581)

Plasmid

#77885

Purpose

3rd generation lentiviral gRNA plasmid targeting human AK8

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AK8 (AK8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
AK8 gRNA (BRDN0001149279)

Plasmid

#77882

Purpose

3rd generation lentiviral gRNA plasmid targeting human AK8

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AK8 (AK8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
AK8 gRNA (BRDN0001149503)

Plasmid

#77883

Purpose

3rd generation lentiviral gRNA plasmid targeting human AK8

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AK8 (AK8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
AK8 gRNA (BRDN0001146232)

Plasmid

#77884

Purpose

3rd generation lentiviral gRNA plasmid targeting human AK8

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AK8 (AK8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
LRPPRC gRNA (BRDN0001145923)

Plasmid

#77775

Purpose

3rd generation lentiviral gRNA plasmid targeting human LRPPRC

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LRPPRC (LRPPRC Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
FGGY gRNA (BRDN0001147914)

Plasmid

#77110

Purpose

3rd generation lentiviral gRNA plasmid targeting human FGGY

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FGGY (FGGY Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
FGGY gRNA (BRDN0001145455)

Plasmid

#77111

Purpose

3rd generation lentiviral gRNA plasmid targeting human FGGY

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FGGY (FGGY Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
ABCC1 gRNA (BRDN0001144857)

Plasmid

#76858

Purpose

3rd generation lentiviral gRNA plasmid targeting human ABCC1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ABCC1 (ABCC1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
ABCC1 gRNA (BRDN0001144957)

Plasmid

#76859

Purpose

3rd generation lentiviral gRNA plasmid targeting human ABCC1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ABCC1 (ABCC1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2001 - 2040 of 2122 results

Results per page:

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