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pCR3.1-OPNc M4

Plasmid

#106324

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Expression

Mammalian

Mutation

OPNc splice site deletion mutant EEKQNA-->EEKNA

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc M3

Plasmid

#106323

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Expression

Mammalian

Mutation

OPNc mutated splice site EEKQNAV-->AAAAAAA

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pRec-GalS

Plasmid

#104573

Purpose

Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI-GalS chimera constitutively.

Depositor

Harris Wang

Article

Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

Insert

PTet-cas12, tetR, lacI-galS

Use

Synthetic Biology

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pRec-TreR

Plasmid

#104574

Purpose

Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI-TreR chimera constitutively.

Depositor

Harris Wang

Article

Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

Insert

PTet-cas12, tetR, lacI-treR

Use

Synthetic Biology

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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rpaA_NS1

Plasmid

#101829

Purpose

drives expression of rpaA from the NS1 site in the S. elongatus PCC7942 genome

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

RpaA with its native promoter

Use

Cyanobacteria cloning vector

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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P0050::rpaA

Plasmid

#101830

Purpose

drives expression of rpaA from an rpaA-independent promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

RpaA with p0050 promoter (+ gentamicin resistance casette)

Use

Cloning vector

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Ptrc::galP_NS1

Plasmid

#101843

Purpose

drives expression of galP transporter from NS1 locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Ptrc promoter driving GalP transporter expression

Use

Cloning vector

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pJRL2 GAL1(TATA-C2)pr VYFP (EB#1577)

Plasmid

#14851

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

GAL1 promoter TATA-C2 (GAL1 Budding Yeast)

Tags

VYFP

Expression

Yeast

Mutation

TATA-C2. TATA box mutated from TATATAAG to TCTATA…

Available Since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
pJRL2 GAL1(TATA-C2)pr ECFP (EB#1578)

Plasmid

#14852

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

GAL1 promoter TATA-C2 (GAL1 Budding Yeast)

Tags

ECFP

Expression

Yeast

Mutation

TATA-C2. TATA box mutated from TATATAAG to TCTATA…

Available Since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
pLenti_9xD3B_Cluc_Norm

Plasmid

#68414

Purpose

CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," lentiviral format.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

Cypridina Luciferase-2A-Venus YFP

Use

Lentiviral and Luciferase

Mutation

YFP: M153T,V163A,S175G ("Venus") YFP Va…

Promoter

minimalCMV

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCS-APTA

Plasmid

#247077

Purpose

pCS27 containing aroL, ppsA, tktA, aroG(fbr) from E. coli

Depositor

Yajun Yan

Article

Shen et al Metab Eng. 2017 Jul;42:52-58. doi: 10.10

Inserts

aroL
ppsA
tktA
aroG(fbr)

Expression

Bacterial

Mutation

feedback-inhibition-resistant

Promoter

PLlacO1

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAM5505

Plasmid

#132664

Purpose

pRL623-based helper plasmid for conjugations, with RSF1010 mobA gene

Depositor

Susan Golden

Article

Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

Insert

MobA

Expression

Bacterial

Mutation

please see depositor comments below

Promoter

p1,p3

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAM5409

Plasmid

#132662

Purpose

RSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistance

Depositor

Susan Golden

Article

Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

Inserts

aadA
YFP

Expression

Bacterial

Promoter

PaadA and PconII

Available Since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pBbAW4k-loxP-TT-loxP-mRFP1

Plasmid

#134405

Purpose

Fluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.

Depositor

Mary Dunlop

Article

Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

Insert

W4-loxP-TT-loxP-mRFP1

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

W4 (modified T7A1)

Available Since

Jan. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-KIRIN1-GR

Plasmid

#107417

Purpose

Bacterial Expression plasmid for potassium ion ratiometric indicator KIRIN1-GR

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

KIRIN1-GR

Expression

Bacterial

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAM5411

Plasmid

#132663

Purpose

RSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistance

Depositor

Susan Golden

Article

Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

Inserts

aadA
YFP

Expression

Bacterial

Promoter

PaadA and PconII

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCambia-PUP-IT

Plasmid

#186478

Purpose

Expresses POI-PUP-IT fusion in Plants

Depositor

Jen Sheen

Article

Ye et al Nature. 2022 Sep 14. pii: 10.1038/s41586

Insert

FLAG-Pup(E)

Tags

Triple Flag Tag

Expression

Plant

Promoter

35S

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-jRCaMP1a

Plasmid

#113936

Purpose

Bacterial expression of red fluorescent Ca2+ sensor jRCaMP1a

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

jRCaMP1a

Tags

6xHis

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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Beta-arrestin2 GFP WT

Plasmid

#35411

Depositor

Robert Lefkowitz

Article

Shenoy et al J Biol Chem. 2005 Apr 15;280(15):15315-2

Insert

βarrestin2 (Arrb2 Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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pBad-HisB-GRvT

Plasmid

#87363

Purpose

Expresses GRvT, a tandem heterodimer green and red fluorescent protein

Depositor

Robert Campbell

Article

Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

Insert

GRvT

Tags

His-Tag

Expression

Bacterial

Promoter

AraBad

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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