We narrowed to 22,185 results for: TES

1. pcDNA-dCas9-HA

   Plasmid

   #61355

   Purpose

   encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal HA tag
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag‐mTim3

   Plasmid

   #49208

   Purpose

   expresses Flag tagged mouse Tim3

   Depositor

   Lawrence Kane

   Article

   Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

   Insert

   Tim3 (Havcr2 Mouse)
   

   Tags

   FLAG and signal sequence

   Expression

   Mammalian

   Mutation

   no Tim3 signal sequence

   Promoter

   EF1a

   Available Since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-ExRai-AKAR2

   Plasmid

   #161753

   Purpose

   Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase A activity in living cells and in vivo.

   Depositor

   Jin Zhang

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

   Insert

   ExRai-AKAR2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-CMV-Luc2-IRES-ZsGreen-W

   Plasmid

   #164432

   Purpose

   Lentiviral vector backbone encoding firefly luciferase and ZsGreen

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Inserts

   Luciferase
   ZsGreen
   

   Use

   Lentiviral

   Available Since

   Feb. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-dCas9-VP64

   Plasmid

   #47107

   Purpose

   Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   dCas9-VP64
   

   Use

   CRISPR

   Tags

   Flag, HA, SV40 NLS, and VP64

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHDM-Hgpm2

   Plasmid

   #164441

   Purpose

   Encodes codon-optimized HIV gagpol for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   Codon Optimized HIV-1 GagPol
   

   Expression

   Mammalian

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPINK-TRABID (OTU+AnkUBD, aa 245-697)

   Plasmid

   #61590

   Purpose

   Expresses human TRABID (OTU+AnkUBD domains) in E. coli.

   Depositor

   David Komander

   Article

   Licchesi et al Nat Struct Mol Biol. 2011 Dec 11;19(1):6

   Insert

   TRABID (ZRANB1 Human)
   

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-244 and aa 698-708.

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. huCaV2.2 (pSAD442-1)

   Plasmid

   #62574

   Purpose

   Human CaV2.2 expression in mammalian cells

   Depositor

   Diane Lipscombe

   Article

   Groen et al Hum Mol Genet. 2015 Feb 15;24(4):987-993

   Insert

   CACNA1B (CACNA1B Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOPINK-OTUD3 (OTU, aa 52-209)

   Plasmid

   #61412

   Purpose

   Expresses human OTUD3 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD3 (OTUD3 Human)
   

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-51 and aa 210-398.

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRC-CMV-Rev1b

    Plasmid

    #164443

    Purpose

    Encodes HIV-1 Rev accessory protein for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    HIV-1 Rev
    

    Expression

    Mammalian

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHDM-VSV-G

    Plasmid

    #164440

    Purpose

    Encodes VSV-G protein for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    VSV-G
    

    Expression

    Mammalian

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-dCas9-KRAB-PGK-HygR

    Plasmid

    #83890

    Purpose

    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Mutation

    D10A and H840A

    Promoter

    Human Ubiquitin C Promoter and mouse phosphoglyce…

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHDM-Tat1b

    Plasmid

    #164442

    Purpose

    Encodes HIV-1 Tat accessory protein for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    HIV-1 Tat
    

    Expression

    Mammalian

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOPINK-OTUD6B (full-length, aa 1-293)

    Plasmid

    #61418

    Purpose

    Expresses human OTUD6B (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD6B (OTUD6B Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-pmExRai-AKAR2

    Plasmid

    #161755

    Purpose

    Plasma membrane-targeted ExRai-AKAR2.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    Lyn-ExRai-AKAR2
    

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn1-DIO-ExRai-AKAR2

    Plasmid

    #171846

    Purpose

    Cre-dependent, synapsin promoter-driven expression of the green fluorescent ExRai-AKAR2 PKA biosensor in neurons.

    Depositor

    Richard Huganir

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    ExRai-AKAR2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn1

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro

    Plasmid

    #132771

    Purpose

    Interacts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.

    Depositor

    Jan Lammerding

    Article

    Denais et al Science. 2016 Apr 15;352(6283):353-8. do

    Insert

    Cyclic GMP-AMP synthase (CGAS Human)
    

    Use

    Lentiviral

    Tags

    mCherry2

    Expression

    Mammalian

    Mutation

    E225A D227A

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-mitoExRai-AKAR2

    Plasmid

    #161757

    Purpose

    ExRai-AKAR2 targeted to the outer mitochondrial membrane.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    DAKAP-ExRai-AKAR2
    

    Tags

    N-terminal 30 amino acids from DAKAP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-Miro1 S156A

    Plasmid

    #127588

    Purpose

    Phospho-mutant of S156

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Miro1 (RHOT1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S156A

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA 3.1 AnxA6

    Plasmid

    #20407

    Depositor

    Carlos Enrich

    Article

    Vila de Muga et al Oncogene. 2009 Jan 22. 28(3):363-77.

    Insert

    AnxA6 (Anxa6 Rat)
    

    Expression

    Mammalian

    Available Since

    May 1, 2009

    Availability

    Academic Institutions and Nonprofits only