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Plasmid#196868PurposeExpression of Pericentrin-AKAP450 centrosomal targeting (PACT) domain fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). PACT targets CM1 to the centrosomeDepositorInsertPACT-mOrange2-hCM1 (Akap9 Rat)
UseTagsExpressionMammalianMutationPromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMX-mPGK-CD90.2-Rluc_miR
Plasmid#163332PurposeRetroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the murine PGK promoter.DepositorInsertCD90.2 (Thy1 Mouse)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromotermPGKAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMY-CD90.2-Rluc_miR
Plasmid#163333PurposeRetroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the LTR promoter.DepositorInsertCD90.2 (Thy1 Mouse)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMX-CAG-CD90.2-Rluc_miR
Plasmid#163331PurposeRetroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the CAG promoter.DepositorInsertCD90.2 (Thy1 Mouse)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMX-hFTH1-CD90.2-Rluc_miR
Plasmid#163330PurposeRetroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the human FTH1 promoter.DepositorInsertCD90.2 (Thy1 Mouse)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterhFTH1Available sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMX-hPGK-CD90.2-Rluc_miR
Plasmid#163329PurposeRetroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the human PGK promoter.DepositorInsertCD90.2 (Thy1 Mouse)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterhPGKAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pJK709
Plasmid#72272PurposeExpression of Gluconacetobacter diazotrophicus succinyl-CoA:acetate CoA-transferase (synthetic gene)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-EWSR1 No Stop
Plasmid#84888PurposeDONOR vector for Gateway cloning of EWSR1 No StopDepositorInsertEWSR1 (EWSR1 Human)
UseGateway donorTagsExpressionMutationno stopPromoternoneAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-EWSR1 Stop
Plasmid#84887PurposeDONOR vector for Gateway cloning of EWSR1DepositorInsertEWSR1 (EWSR1 Human)
UseGateway donorTagsExpressionMutationPromoternoneAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-EWSR1 g1750a No Stop
Plasmid#84894PurposeDONOR vector for Gateway cloning of EWSR1 g1750a No StopDepositorInsertEWSR1 (EWSR1 Human)
UseGateway donorTagsExpressionMutationg1750a No StopPromoternoneAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-EWSR1 g1750a Stop
Plasmid#84893PurposeDONOR vector for Gateway cloning of EWSR1 g1750a StopDepositorInsertEWSR1 (EWSR1 Human)
UseGateway donorTagsExpressionMutationg1750aPromoternoneAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-EWSR1 c1655t No Stop
Plasmid#84892PurposeDONOR vector for Gateway cloning of EWSR1 c1655t No StopDepositorInsertEWSR1 (EWSR1 Human)
UseGateway donorTagsExpressionMutationc1655t No StopPromoternoneAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-EWSR1 c1655t Stop
Plasmid#84891PurposeDONOR vector for Gateway cloning of EWSR1 c1655t StopDepositorInsertEWSR1 (EWSR1 Human)
UseGateway donorTagsExpressionMutationc1655tPromoternoneAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-EWSR1 g1532c No Stop
Plasmid#84890PurposeDONOR vector for Gateway cloning of EWSR1 g1532c No StopDepositorInsertEWSR1 (EWSR1 Human)
UseGateway donorTagsExpressionMutationg1532c No StopPromoternoneAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pLXIN2-RFP
Plasmid#99205PurposeRFP expressing bicistronic retroviral vectorDepositorInsertRed fluorescent protein
UseRetroviral; Bicistronic retroviral expression vec…TagsExpressionMammalianMutationThe dsRed cDNA was PCR amplified off AddGene plas…PromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBABE.zeo/TR
Plasmid#110358PurposeMammalian retroviral expression of Tetracycline repressor with β-globin intron for doxycycline inducible systemsDepositorInsertTetracycline repressor
UseRetroviralTagsExpressionMammalianMutationPromotergag truncatedAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-NFLAG-hEWSR1WT-CMyc
Plasmid#50733PurposeProduces lentivirus expressing human EWSR1 wild type with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2GDepositorInsertEWSR1 (EWSR1 Human)
UseLentiviralTagsFLAG and MycExpressionMutationPromoterTREAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-NFLAG-hEWSR1G511A-CMyc
Plasmid#50734PurposeProduces lentivirus expressing human EWSR1G511A mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2GDepositorInsertEWSR1 G511A (EWSR1 Human)
UseLentiviralTagsFLAG and MycExpressionMutationChanged Gly 511 to AlaPromoterTREAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only