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pCMV-pro-Il1b-C-Flag

Plasmid

#75131

Purpose

Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

interleukin 1 beta (Il1b Mouse)

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

(please see depositor comments below)

Promoter

CMV

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-NLRP3 711-1034

Plasmid

#75141

Purpose

Expresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the LRR domain) in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NLR family, pyrin domain containing 3 (Nlrp3 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

fragment encoding amino acids 711-1034

Promoter

CMV

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-NLRP3 91-710

Plasmid

#75140

Purpose

Expresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NLR family, pyrin domain containing 3 (Nlrp3 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

fragment encoding amino acids 91-710

Promoter

CMV

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-sgRab18.N-Cas9-T2A-mCherry-P2A-Puro

Plasmid

#129418

Purpose

Encoding Cas9 and sgRAB18.N for CRISPR/Cas9 mediated HDR tagging of endogenous human RAB18 N-terminus

Depositor

Elina Ikonen

Article

Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

Insert

SpCas9 and sgRAB18.N (RAB18 Human)

Use

CRISPR

Tags

T2A-mCherry-P2A-Puro

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL.RBBP4

Plasmid

#125173

Purpose

Expresses human RBBP4 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Histone-binding protein RBBP4 (RBBP4 Human)

Use

Tags

His-TEV site

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pUltraHot-mCherry-BLM-WT

Plasmid

#206966

Purpose

expressing the protein BLM fused to mCherry in human cells

Depositor

Eran Hornstein

Article

Danino et al Nucleic Acids Res. 2023 Jul 28:gkad613.

Insert

Bloom Syndrome RecQ Like Helicase (BLM Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pFastbac-Flag-BRCA1

Plasmid

#223228

Purpose

Insect expression construct for Flag-tagged BRCA1 protein production and purification

Depositor

Weixing Zhao

Article

Wang et al Mol Cell. 2023 Oct 19;83(20):3679-3691.e

Insert

Breast Cancer 1 (BRCA1 Human)

Use

Tags

Flag

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL.EED

Plasmid

#125174

Purpose

Expresses human EED in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Polycomb protein EED (EED Human)

Use

Tags

His-TEV

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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FUW TetO HA-AMPKa2 WT

Plasmid

#69826

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter with tetracycline operator

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL.EZH2

Plasmid

#125171

Purpose

Expresses human EZH2 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

EZH2 (EZH2 Human)

Use

Tags

His-TEV

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-sgACSL3.C-Cas9-P2A-Puro

Plasmid

#129412

Purpose

Encoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminus

Depositor

Elina Ikonen

Article

Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

Insert

SpCas9 and sgACSL3.C (ACSL3 Human)

Use

CRISPR

Tags

P2A-Puro

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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FUW TetO HA-AMPKa2 AS

Plasmid

#69827

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter with tetracycline operator

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-ACSL3-sfGFP(C) HDR template

Plasmid

#129413

Purpose

HDR tempalte for tagging of endogenous human ACSL3 C-terminus with sfGFP

Depositor

Elina Ikonen

Article

Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

Insert

ACSL3 HDR template (ACSL3 Human)

Use

CRISPR and TALEN; Endogenous tagging hdr template

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLVTHM-Bptf-sh28

Plasmid

#83278

Purpose

shRNA targeting BPTF

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA #28 to BPTF (BPTF, Bptf Human, Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE-H1

Available since

Oct. 25, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pLVTHM-Bptf-sh5

Plasmid

#83277

Purpose

shRNA targeting BPTF

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA #5 to BPTF (BPTF, Bptf Human, Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE-H1

Available since

Oct. 25, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pSIREN-RetroQ-BPTF-sh3

Plasmid

#73667

Purpose

pSIREN-RetroQ vector containing shRNA sequence to BPTF

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA #3 to BPTF (BPTF, Bptf Human, Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

March 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pU6-crRNA(SOD1)

Plasmid

#109321

Purpose

AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human SOD1

Depositor

Feng Zhang

Article

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

mCherry-KASH (SOD1 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn1

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual His6-TEV-CUL2, Rbx1

Plasmid

#231000

Purpose

Dual expression vector containing mouse CUL2 and Rbx1 for protein puritification from SF9 insect cells

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Inserts

Rbx1 (Rbx1 Mouse)
CUL2 (Cul2 Mouse)

Use

Tags

6x HIS

Expression

Insect

Mutation

Promoter

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL.RING1B

Plasmid

#224712

Purpose

Expresses N-terminal hexahistidine-tagged human RING1B (RNF2) in insect cells.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

RING1b (RNF2 Human)

Use

Tags

6x histidine tag

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Oct. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL.BMI1

Plasmid

#224711

Purpose

Expresses N-terminal hexahistidine-tagged human BMI1 (PCGF4) in insect cells.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

BMI1 (BMI1 Human)

Use

Tags

6x histidine tag

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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