We narrowed to 5,093 results for: puc

1. MoClo adapted pUC57-Amp Level 1 position 5

   Plasmid

   #109224

   Purpose

   Non binary level 1 position 5 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plants

   Depositor

   Elena Caro Bernat

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. MoClo adapted pUC57-Amp Level 1 position 7

   Plasmid

   #113044

   Purpose

   Non binary level 1 position 7 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plants

   Depositor

   Elena Caro Bernat

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. MoClo adapted pUC57-Amp Level 1 position 3

   Plasmid

   #109222

   Purpose

   Non binary level 1 position 3 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plants

   Depositor

   Elena Caro Bernat

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro

   Plasmid

   #89991

   Purpose

   Donor template for generation of SOX2-ntdTomato reporter cell lines

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

   Insert

   SOX2-T2A-2xNLS-TdTomato-F2A-Puro (SOX2 Human)
   

   Use

   CRISPR

   Promoter

   none

   Available Since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro

   Plasmid

   #89992

   Purpose

   Donor template for generation of OCT4-T2A-nEmGFP reporter cell lines

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Res. 2017 Aug;23:105-108. doi:

   Insert

   OCT4-T2A-NLS-EmGFP-P2A-Puro (POU5F1 Human)
   

   Use

   CRISPR

   Promoter

   none

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-TgKI-MCS-p2A-mScarlet-MCS-polyA

   Plasmid

   #174029

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   p2A-mScarlet
   

   Use

   Zebrafish knock-in tagging

   Available Since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJM230 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-lacZ)

   Plasmid

   #110560

   Purpose

   miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-lacZ insert

   Depositor

   Joanna Goldberg

   Article

   Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

   Insert

   stRBS-lacZ
   

   Expression

   Bacterial

   Promoter

   rhaSR-PrhaBAD

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-TgKI-MCS-p2A-eGFP-MCS-polyA

   Plasmid

   #174028

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   p2A-eGFP
   

   Use

   Zebrafish knock-in tagging

   Available Since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Padh1-OsTIR1(F74A)-NLS-TADH1-Sp.arg3+/pUC19*

   Plasmid

   #169353

   Purpose

   Expression of OsTIR1(F74A) in S. pombe from adh1 promoter, integrated at arg3-D4 locus

   Depositor

   Tony Carr

   Insert

   OsTIR1(F74A)
   

   Expression

   Yeast

   Mutation

   F74A

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC57-2xFLAG-mAID-AMBRA1 HDR donor template

    Plasmid

    #172609

    Purpose

    Donor plasmid containing the HDR template for the in-frame, N-terminal 2xFLAG-mAID tagging of AMBRA1 gene

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    2xFLAG-mAID-AMBRA1 (AMBRA1 Synthetic, Human)
    

    Use

    CRISPR; Human targeting

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. lacI-Ptac-lacO-BsaI-BsaI-IMBB4-pUC

    Plasmid

    #170772

    Purpose

    test plasmid for Golden Gate assembly, expresses lacI repressor

    Depositor

    Ichiro Matsumura

    Article

    Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

    Insert

    lacI
    

    Promoter

    lacI promoter

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJM240 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-aacC1)

    Plasmid

    #110561

    Purpose

    miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insert

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    stRBS-aacC1
    

    Expression

    Bacterial

    Promoter

    rhaSR-PrhaBAD

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 D141N

    Plasmid

    #222650

    Purpose

    For in vitro transcription of mouse Zc3h12a D141N mutant tagged with FLAG-HA at N-term

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12a (Zc3h12a Mouse)
    

    Use

    In vitro transcription

    Tags

    FLAG and HA

    Mutation

    mutated aspartic acid 141 to asparagine to abroga…

    Promoter

    T7

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320)

    Plasmid

    #208977

    Purpose

    Entry vector for human U6 promoter driven SpCas9-based pegRNAs, comprised of hU6-[BsmBI]-terminator (spacer and RTT/PBS oligos must be cloned in)

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    pUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19-TgKI-MCS-p2A-QF2-MCS-polyA

    Plasmid

    #174026

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    p2A-QF2
    

    Use

    Zebrafish knock-in tagging

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19-TgKI-MCS-p2A-Venus-PEST-MCS-polyA

    Plasmid

    #174030

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    p2A-Venus-PEST
    

    Use

    Zebrafish knock-in tagging

    Available Since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC57-ITR2-H1-sgCTG-SlugD10A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #222857

    Purpose

    This plasmid codes for the Slug-KH nickase with a sg(CTG)6

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    Slug-KHD10A+sgCTG
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, ITR2 and ITR2

    Expression

    Mammalian

    Mutation

    KH variant and D10A mutation

    Promoter

    H1 and eCMV

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC57-ITR2-H1-sgAGC-SlugD10A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #222859

    Purpose

    This plasmid codes for the Slug-KH nickase with a sg(AGC)6

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    Slug-KHD10A+sgAGC
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, ITR2 and ITR2

    Expression

    Mammalian

    Mutation

    KH variant and D10A mutation

    Promoter

    H1 and eCMV

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC57-ITR2-H1-sgCAG-SlugD10A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #222858

    Purpose

    This plasmid codes for the Slug-KH nickase with a sg(CAG)6

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    Slug-KHD10A+sgCAG
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, ITR2 and ITR2

    Expression

    Mammalian

    Mutation

    KH variant and D10A mutation

    Promoter

    H1 and eCMV

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC57-ITR2-H1-sgGCA-SlugD10A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #222860

    Purpose

    This plasmid codes for the Slug-KH nickase with a sg(GCA)6

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    Slug-KHD10A+sgGCA
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, ITR2 and ITR2

    Expression

    Mammalian

    Mutation

    KH variant and D10A mutation

    Promoter

    H1 and eCMV

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge