We narrowed to 28,953 results for: tat
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Plasmid#126698Purposeto express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequenceDepositorInsertsgRNA targeting human SCGB3A2 locus
ExpressionMammalianPromoterHuman U6Available SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_SST1.0
Plasmid#208666PurposeExpresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in a cre-dependent mannerDepositorInsertGPCR activation based somatostatin (SST) sensor GRAB_SST1.0
UseAAVPromoterhSynAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-PHD2
Plasmid#223552PurposeLentivirus transfer plasmid for expression of full length human PHD2DepositorAvailable SinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
APEX2-6xHp
Plasmid#214411PurposeLipid droplet localization of APEX2-6xHp for electron microscopyDepositorAvailable SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
FL-BCL2L1
Plasmid#78860PurposeDULIP positive control (prey) for assay establishmentDepositorInsertBCL2 like 1 (BCL2L1 Human)
UseExpression vectorTagsFirefly luciferase, V5-tagExpressionMammalianPromoterCMVAvailable SinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
FCK-Chronos-GFP
Plasmid#80902PurposeLenti-mediated expression of Chronos-GFP under the CaMKII promoter.DepositorInsertChronos-GFP
UseLentiviralTagsGFPExpressionMammalianPromoterCaMKIIAvailable SinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mPCSK3 H194L
Plasmid#122689PurposeExpresses mutated mouse PCSK3 in mammalian cells (histidine at position 194 mutated to leucine via site directed mutagenesis)DepositorInsertMus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
ExpressionMammalianMutationChanged Histidine 194 to LeucineAvailable SinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Sox17
Plasmid#128828Purposedox-dependent expression of mouse Sox17DepositorInsertSox17 (Sox17 Mouse)
UseLentiviralAvailable SinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBJG1 T7-8xHis-OGT D554N
Plasmid#154279PurposeBacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
Tags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationAspartate 554 changed to Asparagine- bacterial co…Available SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
α-catenin ECFP-YPET FRET ΔF-actin binding site
Plasmid#198245PurposeAlpha catenin FRET conformation sensor protein with C-terminal truncation removing ability to bind F-actin.DepositorInsertα-catenin ECFP-YPET FRET sensor ΔF-actin binding site
TagsECFP and YPETExpressionMammalianMutationDeleted F-actin binding site on a-catenin (849-90…PromoterCMVAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-JAK1Gly590Arg
Plasmid#174573PurposeHuman FLAG-tagged JAK1 Gly590Arg mutant expression (N-term. tag) (CMV prom.)DepositorInsertJAK1Gly590Arg
TagsFLAGExpressionMammalianMutationGly590ArgPromoterCMVAvailable SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-CAD
Plasmid#73567PurposeSoluble activator of Orai1 channelsDepositorAvailable SinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
LUC2 (L/Sec/deltaP)
Plasmid#112145PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with Cysteine 258 mutated to selenocysteine (U) and deleted AUGA in PHGPx SECIS core (Gpx4 Rat, Photinus pyralis)
ExpressionMammalianMutationCysteine 258 mutated to selenocysteine (U) and d…Available SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-RVR
Plasmid#108861PurposeLbCpf1 Gateway entry plasmid with G548R, K554V and Y558R mutationsDepositorInsertLbCpf1-RVR (LbCpf1 with G548R, K554V and Y558R mutations)
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationG548R, K554V and Y558R, Cpf1 is rice codon optimi…Available SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHub10-His-YFP-PIP2;1
Plasmid#99386Purposeoverexpression and isolation of recombinant At-PIP2;1 proteinDepositorAvailable SinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (AA 366-852)
Plasmid#119315Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 366-852 in mammalian cellsDepositorAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.E2.ChETA
Plasmid#220310PurposeDirects ChETA optogenetic actuator to fast spiking interneurons in cortex. Allows for single APs to be evoked.DepositorInsertChETA
UseAAVExpressionMammalianPromoterS5E2Available SinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-shEsr2
Plasmid#120722PurposeLentiviral vector that expresses GFP and an shRNA targeting Esr2 (in pLL3.7).DepositorAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenLox_U6 Nlgn2
Plasmid#59358PurposeLentiviral expression vector: Neuroligin 2 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.DepositorUseLentiviralExpressionMammalianPromoterCAG and mouse U6Available SinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Venus-Noxa-4E-pEGFP-C1
Plasmid#166745PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, Noxa with mutation in BH3 domain to disrupt binding anti-apoptotic proteinsDepositorInsertNoxa-4E (PMAIP1 Human)
TagsVenusExpressionMammalianMutation4 hydrophobic residues in the BH3 region mutated …PromoterCMVAvailable SinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only