We narrowed to 59,496 results for: %s

1. pCMV-CRT-Fc N

   Plasmid

   #221854

   Purpose

   Express human Calreticulin truncated N domain with C-terminal human IgG1Fc tag in mammalian cell.

   Depositor

   Judy Lieberman

   Article

   Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

   Insert

   Calreticulin (CALR Human)
   

   Tags

   Human IgG1Fc tag

   Expression

   Mammalian

   Mutation

   Expresses N domain

   Available Since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-CRT-Fc C

   Plasmid

   #221856

   Purpose

   Express human Calreticulin truncated C domain with C-terminal human IgG1Fc tag in mammalian cell.

   Depositor

   Judy Lieberman

   Article

   Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

   Insert

   Calreticulin (CALR Human)
   

   Tags

   Human IgG1Fc tag

   Expression

   Mammalian

   Mutation

   Expresses C domain

   Available Since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. R620-M19-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP K417T/E484K/N501Y

   Plasmid

   #170203

   Purpose

   Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.28 variant

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S SARS-CoV-2)
   

   Tags

   3C-His8-SBP

   Expression

   Mammalian

   Mutation

   K417T, E484K, N501Y

   Promoter

   CMV51

   Available Since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. R620-M08-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP E484K

   Plasmid

   #170194

   Purpose

   Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), E484K mutant

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S SARS-CoV-2)
   

   Tags

   3C-His8-SBP

   Expression

   Mammalian

   Mutation

   E484K

   Promoter

   CMV51

   Available Since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. R620-M05-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 K417N/E484K/N501Y

   Plasmid

   #170191

   Purpose

   Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), triple mutant from B.1.351 variant

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S SARS-CoV-2)
   

   Tags

   His6

   Expression

   Mammalian

   Mutation

   K417N, E484K, N501Y

   Promoter

   CMV51

   Available Since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT3EF1aH-myr-Akt

   Plasmid

   #179909

   Purpose

   This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.

   Depositor

   Xin Chen

   Article

   Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:

   Insert

   Akt1 (Akt1 Mouse)
   

   Tags

   HA and Myr

   Expression

   Mammalian

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMO14

   Plasmid

   #235736

   Purpose

   Expression of human VPS34 complex II in mammalian cells

   Depositor

   Roger Williams

   Article

   Mercer et al EMBO J. 2021 Jul 15;40(14):e105985. doi:

   Inserts

   UVRAG (UVRAG Human)
   EGFP
   BECN1 (BECN1 Human)
   PIK3C3 (PIK3C3 Human)
   PIK3R4 (PIK3R4 Human)
   

   Tags

   2xStrep-tagII-2xTEV-ZZ

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. P3-dCas9-Tet1-GFP-Puro

   Plasmid

   #190728

   Purpose

   Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

   Depositor

   Maja Jagodic

   Article

   Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

   Insert

   dCas9-tet1
   

   Use

   CRISPR

   Tags

   3XFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Changed Pro434 codon from CCG to CCC and Gly468 f…

   Promoter

   pCAG

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRSF_his6_sumo_OC43_NSP14

   Plasmid

   #213519

   Purpose

   Expresses NSP14 of hCoV OC43

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   hCoV OC43 NSP14 (ORF1ab hCoV OC43)
   

   Tags

   His-SUMO tag

   Expression

   Bacterial

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRSF_his6_sumo_NL63_NSP14

    Plasmid

    #213520

    Purpose

    Expresses NSP14 of hCoV NL63

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    hCoV NL63 NSP14 (ORF1ab hCoV NL63)
    

    Tags

    His6-sumo

    Expression

    Bacterial

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRSF_his6_sumo_229E_NSP14

    Plasmid

    #213521

    Purpose

    Expresses NSP14 of hCoV 229E

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    hCoV 229E NSP14
    

    Tags

    His6-sumo

    Expression

    Bacterial

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDisplay-iSeroSnFR

    Plasmid

    #128484

    Purpose

    Fluorescent reporter for serotonin (mammalian expression, membrane localization tag)

    Depositor

    Lin Tian

    Article

    Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

    Insert

    iSeroSnFR
    

    Tags

    Ig-kappa leader, Myc, PDGFR + enhanced ER export,…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLD-puro-Cc-SARS-CoV-2_NSP16-VA

    Plasmid

    #191215

    Purpose

    Lentiviral expression of SARS-CoV-2 NSP16 in mammalian cells

    Depositor

    Mohan Babu

    Article

    Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

    Insert

    Non structural protein 16 (ORF1ab SARS CoV-2)
    

    Use

    Lentiviral

    Tags

    VA tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pmCherry-TEAD4-YBD

    Plasmid

    #166450

    Purpose

    Expresses fusion of mCherry and TEAD4-YAP binding domain

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mCherry, TEAD4-YBD(217-434) (TEAD4, YAP1 Human)
    

    Expression

    Mammalian

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. peGFP NOVA2 op

    Plasmid

    #224354

    Purpose

    Express GFP-tagged human NOVA2 (codon optimized)

    Depositor

    Nicolas Charlet-Berguerand

    Article

    Mattioli et al Am J Hum Genet. 2020 Apr 2;106(4):438-45

    Insert

    NOVA2 (NOVA2 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    codon optimized for expression in human cells

    Promoter

    CMV and T7

    Available Since

    Sept. 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pAAV-CAG-FLEX-SomaGCaMP7

    Plasmid

    #182942

    Purpose

    Cre-dependent expression of cell body-targeted GCaMP7f, under CAG promoter. As bright as conventional GCaMP7f.

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Insert

    Soma-GCaMP7
    

    Use

    AAV

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CAG-iSeroSnFR-Nlgn

    Plasmid

    #128485

    Purpose

    Fluorescent reporter for serotonin (dendrite localization tag)

    Depositor

    Lin Tian

    Article

    Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

    Has Service

    AAV9

    Insert

    iSeroSnFR
    

    Use

    AAV

    Tags

    Full-length neuroligin, Ig-kappa leader, and cpsf…

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR-TARDBP-A315T

    Plasmid

    #141324

    Purpose

    Gateway cloning of TARDBP-A315T

    Depositor

    Mohan Babu

    Article

    Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

    Insert

    TARDBP-A315T (TARDBP Human)
    

    Use

    Gateway cloning

    Mutation

    A315T

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMVtrunc beta-actinh7TAA-msfGFPΔN7ΔC11-SSSSactin

    Plasmid

    #231549

    Purpose

    Mammalian expression of human β-actin fused intramolecularly to N- and C-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    ACTB (ACTB Human)
    

    Tags

    msfGFP (intramolecular)

    Expression

    Mammalian

    Promoter

    CMVtrunc

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMVtrunc msfGFPΔC10-beta-actin

    Plasmid

    #231548

    Purpose

    Mammalian expression of human beta actin fused to C-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    ACTB (ACTB Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Promoter

    CMVtrunc

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge