We narrowed to 19,160 results for: Tec
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Plasmid#170336PurposeCFP(ST)_EPACdDEP _Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertCFP(ST)_EPACdDEP _Y(UST) (RAPGEF3 Human)
ExpressionMammalianMutationDeletion of DEP domainPromoterCMVAvailable SinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-N1-EXO1b-D173A
Plasmid#111622PurposeExpression of human catalitic dead Exonuclease 1 (EXO1) mCherry-tagged in mammalian cellsDepositorAvailable SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF574
Plasmid#88644PurposeDonor Vector containing ZNF574 transcription factor, part of the Human TFome CollectionDepositorInsertZNF574 (ZNF574 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
SPACE-VRQR (pRZ5137)
Plasmid#140245PurposeCMV promoter expression plasmid for TadA*(V82G)-nCas9_VRQR-pmCDA1(R187W)-UGI-UGI-P2A-EGFPDepositorInsertSPACE_VRQR
ExpressionMammalianMutationV82G in TadA*, VRQR mutations in SpCas9, D10A in …PromoterCMVAvailable SinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAuroraA GFP-AURKA-GFP
Plasmid#157765PurposeExpression of AURKA fused to GFP at both ends in mammalian cells under AurkA promoterDepositorInsertAURKA (AURKA Human)
TagsGFPExpressionMammalianMutationpromoter CMV replaced by AurkA promoterPromoterAURKA minimalAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT410 AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA
Plasmid#202059PurposeInducible AAV activatorDepositorInsertAAV-Rapamycin Inducible-NFZ-Citrine with bGH pA
UseAAV; Aav with reporterExpressionMammalianMutationITRs intactAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-GAL4-IL35-IRES-mCherry-PGK-BFP
Plasmid#223210PurposeLentiviral vector - mCherry reporter and IL35 for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorAvailable SinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-GAL4-PDL1-IRES-mCherry-PGK-BFP
Plasmid#223208PurposeLentiviral vector - mCherry reporter and PDL1 for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorAvailable SinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
RPS6KA3 gRNA (BRDN0001147852)
Plasmid#75944Purpose3rd generation lentiviral gRNA plasmid targeting human RPS6KA3DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJFA274
Plasmid#49239PurposeTET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cellsDepositorInsertTET1 tet methylcytosine dioxygenase 1 (TET1 Human, Synthetic)
UseTALEN; Tale-tet1 expression vectorPromoterEF1aAvailable SinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
TV-hPITX3-Reverse
Plasmid#22208DepositorAvailable SinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ZNF251
Plasmid#101451PurposeDonor Vector containing ZNF251 transcription factor, part of the Human TFome CollectionDepositorInsertZNF251 (ZNF251 Human)
UseGateway shuttling vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-B103 (LM2963)
Plasmid#208960PurposeA variant CE1 construct with B103 DNA polymerase, expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-B103-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A)PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-PolBeta (JO574)
Plasmid#208953PurposeA variant CE1 construct with human DNA polymerase beta, expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-PolBeta-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A)PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
SPACE-ΔUGI (pRZ1836)
Plasmid#140243PurposeCMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFPDepositorInsertSPACE-ΔUGI
ExpressionMammalianMutationV82G in TadA*, D10A in Cas9, R187W in pmCDA1PromoterCMVAvailable SinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF706
Plasmid#88037PurposeDonor Vector containing ZNF706 transcription factor, part of the Human TFome CollectionDepositorInsertZNF706 (ZNF706 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
CSNK1G2 gRNA (BRDN0001162501)
Plasmid#76988Purpose3rd generation lentiviral gRNA plasmid targeting human CSNK1G2DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CSNK1G2 gRNA (BRDN0001149101)
Plasmid#76989Purpose3rd generation lentiviral gRNA plasmid targeting human CSNK1G2DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CSNK1G2 gRNA (BRDN0001145026)
Plasmid#76990Purpose3rd generation lentiviral gRNA plasmid targeting human CSNK1G2DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha2 P35S:dCas9:VPR:Tnos (GB1826)
Plasmid#160623PurposeTU for the constitutive expression of dCas9 fused to VPR (VP64-p65-Rta) activation domainsDepositorInsertP35S:dCas9:VPR-Tnos
ExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only