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pXRA2

Plasmid

#63144

Purpose

ADE2 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

Depositor

Marc Gartenberg

Article

Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

Type

Empty backbone

Use

Cre/Lox

Expression

Bacterial and Yeast

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2 Pc-gE

Plasmid

#51256

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Glu

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 216,220,224,230,248 mutated to Glu

Promoter

EF1a

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pMXs-c-Myc

Plasmid

#13375

Purpose

Retroviral expression of mouse c-Myc

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

myelocytomatosis oncogene (Myc Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-dCas9

Plasmid

#112233

Purpose

Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2

Depositor

Thomas Gilmore

Article

Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do

Inserts

dCas9
Porumycin resistance

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Changed: Aspartic acid 10 to Glycine, Histidine 8…

Promoter

EFS-NS

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-ERK1

Plasmid

#14747

Depositor

Rony Seger

Article

Yung et al J Biol Chem. 2001 Sep 21. 276(38):35280-

Insert

ERK1 (MAPK3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-γ-tubulin

Plasmid

#136799

Purpose

Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-TUBG (TUBG1 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSDM052

Plasmid

#194341

Purpose

pUC mini-Tn7 vector expressing LseCas13a

Depositor

Joseph Bondy-Denomy

Article

Mendoza et al Nature. 2020 Jan;577(7789):244-248. doi:

Insert

Cas13a(Lse)

Expression

Bacterial

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27A

Plasmid

#89237

Purpose

Expression of GFP-tagged human Rab27A in mammalian cells

Depositor

William Gahl

Article

Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

Insert

human Rab27A (RAB7A Human)

Tags

eGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pHIV-Zsgreen

Plasmid

#18121

Depositor

Bryan Welm, Zena Werb

Article

Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

Insert

Zsgreen

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pCMMP-MCS-IRES-eGFP-P2A-iCas9

Plasmid

#178533

Purpose

For genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cells

Depositor

Ryoji Yao

Article

Okamoto et al STAR Protoc. 2021 Sep 16;2(4):100780. do

Insert

iCaspase9 (CASP9 Human)

Expression

Mammalian

Mutation

FKBP12 F36 to V Truncated Caspase9 (135-416; lack…

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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