We narrowed to 18,496 results for: Met

1. pAAV-CMV-H2B-3xFLAG-2A-G(N2c)

   Plasmid

   #194355

   Purpose

   AAV constitutively expressing nuclear FLAG and Rabies Glycoprotein (G) of CVS-N2c strain

   Depositor

   Marco Tripodi

   Article

   Lee et al Nat Methods. 2023 Apr;20(4):580-589. doi

   Inserts

   H2B-3xFLAG
   Rabies CVS-N2c glycoprotein (G)
   

   Use

   AAV

   Tags

   FLAG

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-ChRger1-TS-EYFP

   Plasmid

   #127243

   Purpose

   High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   ChRger1-TS-EYFP
   

   Use

   AAV

   Tags

   EYFP and SpyTag

   Expression

   Mammalian

   Promoter

   hSyn1

   Available Since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. GluR2_adRNA(1,20,6)-ADAR2(E488Q)

   Plasmid

   #124621

   Purpose

   Expresses adRNA(1,20,6) targeting the RAB7A transcript along with the ADAR2(E488Q)

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

   Insert

   ADAR2(E488Q)
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   E488Q hyperactive mutant

   Promoter

   CMV

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXS-IRES-Blast coUGP2

   Plasmid

   #211531

   Purpose

   Expresses codon optimized UGP2

   Depositor

   Richard Possemato

   Article

   Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

   Insert

   UGP2 (UGP2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. FoxO1 KA

   Plasmid

   #66164

   Purpose

   Expresses FoxO1 with three Lys residues surrounding Ser-253 that are known to be sites of acetylation were replaced by alanine residues.

   Depositor

   Ronald Kahn

   Article

   Jing et al Cell Metab. 2007 Aug . 6(2):105-14.

   Insert

   FoxO1 KA (Foxo1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   K242A, K245A and K262A

   Available Since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pTK47_mCherry-LGN-hardened

   Plasmid

   #46347

   Purpose

   rescues the phenotypes caused by LGN RNAi

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

   Insert

   LGN (GPSM2 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   siRNA resistant, gaGTtGacTgcTcgTTtA; deletion of …

   Promoter

   CMV

   Available Since

   Aug. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. FoxO1 KQ

   Plasmid

   #66165

   Purpose

   Expresses FoxO1 with three Lys residues surrounding Ser-253 that are known to be sites of acetylation were replaced by glutamine residues.

   Depositor

   Ronald Kahn

   Article

   Jing et al Cell Metab. 2007 Aug . 6(2):105-14.

   Insert

   FoxO1 KQ (Foxo1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   K242Q, K245Q and K262Q

   Available Since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pGREAT2

   Plasmid

   #170890

   Purpose

   GoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_NOSt+pNOS_Red-F_NOSt

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   E-Luc
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Plant

   Mutation

   c.375C>T silent mutation to remove BbsI site (…

   Promoter

   p35SoTMV

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3/Myc-DNMT3B2

   Plasmid

   #36942

   Depositor

   Arthur Riggs

   Article

   Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

   Insert

   DNMT3B2 (DNMT3B Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   June 22, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pCHC039 (∆phcA)

    Plasmid

    #226207

    Purpose

    Conjugation knockout plasmid (pK18msB) to delete the C. necator LysR-type transcriptional regulator PhcA.

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for PhcA
    

    Use

    Synthetic Biology

    Available Since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pBMN-AS-HNF4α

    Plasmid

    #139305

    Purpose

    Knocking down of human HNF4α through shRNA and amiRNA targeting HNF4α

    Depositor

    Claes Wadelius

    Article

    Pan et al iScience. 2020 Jan 9:100808. doi: 10.101

    Insert

    shRNA and amiRNA against HNF4α
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFEndoNA

    Plasmid

    #174724

    Purpose

    Expresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.

    Depositor

    Jukka Finne

    Article

    Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

    Insert

    GFP-endoNA fusion protein
    

    Tags

    Histag

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. SEH1L in pRK5

    Plasmid

    #154216

    Purpose

    Untagged SEH1L cDNA expression vector for transfections

    Depositor

    David Sabatini

    Article

    Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

    Insert

    SEH1L (SEH1L Human)
    

    Tags

    NONE

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSNAP-CLC-EYFP

    Plasmid

    #38011

    Depositor

    Xiaowei Zhuang

    Article

    Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Tags

    EYFP and SNAP26m

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Sept. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pet28a-His6-Halo-Tev-Keap1

    Plasmid

    #62456

    Purpose

    His6-halo tagged human Keap1 protein for E.coli expression

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Keap1 (KEAP1 Human)
    

    Tags

    His-Halo-Tev

    Expression

    Bacterial

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a(+)::MBP-NL3F10H

    Plasmid

    #141291

    Purpose

    MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    MBP-NLUC3F10H
    

    Use

    Luciferase

    Tags

    Flag (x3), His (x10), and MBP

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDendra2-H4

    Plasmid

    #157815

    Purpose

    Fluorescent reporter for histone H4 tracking

    Depositor

    Xin Chen

    Article

    Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

    Insert

    H4 (H4c14 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGREAT1

    Plasmid

    #170889

    Purpose

    GoldenBraid Double Transcriptional Unit - pNOS_E-Luc_NOSt+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    pNOS

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBabe-FLAG-Neuroserpin

    Plasmid

    #58261

    Purpose

    Retroviral vector for expressing Neuroserpin in mammalian cells

    Depositor

    Joan Massague

    Article

    Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

    Insert

    Neuroserpin (SERPINI1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pCAG-tagRFP-NLS-HA-Bxb1

    Plasmid

    #65625

    Purpose

    Vector for cotranslational expression of tagRFP-Bxb1

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Bxb1 integrase
    

    Tags

    HA, SV40 NLS, and tagRFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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