We narrowed to 10,989 results for: T7
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Plasmid#224758PurposeIn vitro transcription for Oocyte expression of mTREK-1(tandem S131C-wt)DepositorInsertTandem TREK-1 (S131C-wt) (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionMutationS131CPromoterT7Available SinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(S131C/A286F)
Plasmid#224748PurposeIn vitro transcription for Oocyte expression of mTREK-1(S131C/A286F)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionMutationS131C / A286FPromoterT7Available SinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1 (S131A/C159A/C219A/C365A/C399A)
Plasmid#224756PurposeIn vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A/C365A/C399A)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionMutationS131A/C159A/C219A/C365A/C399APromoterT7Available SinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest17_hnRNPA1_YtoW
Plasmid#224253PurposeBacterial expression of N-terminally 6His-tagged hnRNPA1_YtoWDepositorInserthnRNPA1_YtoW (HNRNPA1 Human)
Tags6xHisExpressionBacterialMutationY52W, Y59W, Y75W, Y81W, Y110W, Y120W, Y129WPromoterT7Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest17_hnRNPA1_allW
Plasmid#224252PurposeBacterial expression of N-terminally 6His-tagged hnRNPA1_allWDepositorInserthnRNPA1_allW (HNRNPA1 Human)
Tags6xHisExpressionBacterialMutationF17W,F25W,F31W,F37W,F43W,Y52W, Y59W, F62W, F69W, …PromoterT7Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCM2(413-438)_pHisSUMO(K0)
Plasmid#224065PurposeBacterial expression of the C-terminal helix of CCM2 (residues 413-438) fused to an N-terminal hexa-histidine tag and SUMO(K0) [all lysines in SUMO mutated to arginines]. HRV-3C cleavage siteDepositorAvailable SinceAug. 26, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21b-KRas/13C-FKBP-MBP
Plasmid#214303PurposeExpress split-KRas fragment fused with FKBP and MBPDepositorInsertSplit-KRas fragment fused with FKBP (KRAS Human)
TagsMaltose-binding protein (MBP)ExpressionBacterialMutationKRas-Q61LPromoterT7Available SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-SAVED-CHAT-cbm
Plasmid#214032PurposeBacterial expression plasmid for strep-tagged SAVED-CHAT cA3-binding pocket mutant from Haliangium ochraceumDepositorInsertSAVED-CHAT
TagsStrep-tag IIExpressionBacterialMutationK121E, Q122A, N274A, R276E, Y285A, F286APromoterT7 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-SAVED-CHAT-sm
Plasmid#214033PurposeBacterial expression plasmid for strep-tagged SAVED-CHAT CHAT-CHAT singlet interface mutant from Haliangium ochraceumDepositorInsertSAVED-CHAT
TagsStrep-tag IIExpressionBacterialMutationR456E, D459K, D476K, R479EPromoterT7 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-dPCaspase
Plasmid#214035PurposeBacterial expression plasmid for strep-tagged catalytically dead PCaspase (Prokaryotic Caspase) from Haliangium ochraceumDepositorInsertPCaspase
TagsStrep-tag IIExpressionBacterialMutationH79A, C146APromoterT7 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-PCaspase-R153A
Plasmid#214036PurposeBacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) R153A mutant from Haliangium ochraceumDepositorInsertPCaspase
TagsStrep-tag IIExpressionBacterialMutationR153APromoterT7 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-DDX5ΔPrD(kf)-SNAP
Plasmid#166150PurposeExpression of N. furzeri DDX5ΔPrD(1-535) mutant in E. coliDepositorInsertDDX5ΔPrD(1-535)
TagsHis10-Smt3 and SNAPExpressionBacterialMutationΔPrD(1-535)PromoterT7Available SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-DDX5ΔIDR(kf)-SNAP
Plasmid#166151PurposeExpression of N. furzeri DDX5ΔIDR(1-483) mutant in E. coliDepositorInsertDDX5ΔIDR(1-483)
TagsHis10-Smt3 and SNAPExpressionBacterialMutationΔIDR(1-483)PromoterT7Available SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-effector-ncRNA-rrt-His8
Plasmid#194089PurposeExpresses Ec86 operon (effector, ncRNA and reverse transcriptase) in Escherichia coliDepositorInsertEc86 effector-ncRNA-rrt
TagsHis8PromoterT7Available SinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
TH1531-DuET
Plasmid#213243PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
AGTR1-DuET
Plasmid#213183PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE ∆238-245
Plasmid#139318PurposeGST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
TagsGSTExpressionBacterialMutationAO7 cloned into the first multiple cloning site o…PromoterT7 promoter-1Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pet28b-His_RAD23A-UBA2
Plasmid#201550PurposeExpresses UBA2 of human RAD23A in E. coliDepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
Tags6x HisExpressionBacterialMutationExpresses amino acids 311-363 of DNA polymerase i…PromoterT7Available SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5A→M_IDR + 5M→A_CR
Plasmid#194268PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with TDP-43 _CTD_5A→M_IDR + 5M→A_CRDepositorInsertTDP-43 (TARDBP Synthetic, Human)
Tags6XHistagExpressionBacterialMutationA297M, A367M, A381M, A388M, A391M M322A, M323A, M…PromoterT7Available SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
K365-iLid
Plasmid#188456PurposeComponent of optically-induced motor dimerization systemDepositorInsertKinesin 1-365 (Khc Fly)
Tags6xHis and iLidExpressionBacterialMutationKinesin amino acids 1-365PromoterT7Available SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only