We narrowed to 18,247 results for: multi
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Plasmid#213344PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMRGPRX1 (MRGPRX1 Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
HTR2C-DuET
Plasmid#213323PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHTR2C (HTR2C Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPRC5B-DuET
Plasmid#213296PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPRC5B (GPRC5B Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CALCRb-DuET
Plasmid#213193PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCALCRb (CALCRL Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FLEX-H2BmTagBFP2-oG
Plasmid#217976PurposeAAV transfer plasmid to express H2B-BFP and codon-optimized Rabies G proteinDepositorInsertsH2BmTagBFP2
oG
UseAAVTagsmTAGBFP2PromoterhSynAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-T7-J1~398-Luc
Plasmid#208639Purposefor in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19DepositorInsertHepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
UseLuciferase; In vitro transcription templateTagsFirefly Luciferase (F-Luc and T7 promoterMutationnt 1~398, in frame with donstream F-LucPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEditA
Plasmid#207531PurposePlasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPlasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA
UseCRISPR; Pseudomonas vectorAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11299: pHR-HSP-ABE8e-hyperdCas12a-EPS-puro
Plasmid#210605Purposeheat shock promoter - activated hyperdCas12a-based transcriptional base editorDepositorInsertABE8e-hyperdCas12a
UseCRISPR and LentiviralPromotertruncated hsp70Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-PDGFRb-HA
Plasmid#204350PurposeExpresses wild-type PDGFRb gene. Used for DNA delivery using Adeno-associated Virus.DepositorAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKPI-MtLAP1
Plasmid#193521PurposeAnthocyanin pigmentation based visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula rootsDepositorInsertsMtLAP1
DsRed
ExpressionPlantPromoterATUBI10 and Medtr8g059790(KPI)Available sinceJune 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2_claudink:Cas9green; control_gRNA
Plasmid#199336PurposepDEL143; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous control sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsclaudink
Cas9
control sgRNA
UseTol2 destination vectorAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEJS1964 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-miniU6-Rosa26
Plasmid#199262PurposeSingle AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one miniU6 driven sgRNA targeting mouse Rosa26 geneDepositorInsertNmeABE8e
UseAAV and CRISPRTagsNLSExpressionMammalianPromoterU1aAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFRT-PonA-BI-Gl-WT-24xMS2-PTC-18xPP7 (WP)
Plasmid#189792PurposeExpress wild-type and PTC-containing beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertbeta-globin (HBB Human, Mouse)
ExpressionMammalianPromoterPonasterone A inducibleAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Phi3-Zsgreen-PGK-mCherry
Plasmid#153320PurposeExpresses zsGreen and mCherry in mammalian cells.DepositorInsertzsGreen
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA
Plasmid#194723Purposebased on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA that target hAAVS1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAsCpf1 (AAVS1 Acidaminococcus sp.)
UseCRISPRExpressionMammalianAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC8_PH-GST-Nter-GWs-Lox (VE5586)
Plasmid#163766PurposeTransfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter GST tag under the pH promoter.DepositorInsertN-terminal GST tag
TagsGST TagExpressionInsectPromoterPHAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked Free ClLIS1 (M)
Plasmid#182487PurposeYeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-ClLIS1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertFPPS(M)-LS
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_LAMP1_STf
Plasmid#182006PurposeCMV overexpression of SNAP-tag fast in the lysosome of mammalian cell linesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLAMP1_STf
ExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Muc5b D1 (murine) with cleavable His tag
Plasmid#181814PurposeExpresses murine Muc5b D1 with cleavable His tag, residues 50-426DepositorInsertMuc5b D1 (Muc5b Mouse)
TagsTEV cleavage site followed by 6xHistidine tagExpressionMammalianPromoterCMVAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-KMS
Plasmid#188038PurposePolycistronic human KLF4, MYC and SOX2 were cloned into the pMXs vectorDepositorUseRetroviralExpressionMammalianAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only