161,256 results

1. pCAG_smFP Myc

   Plasmid

   #59757

   Purpose

   encodes multiple copies of an epitope tag Myc

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Insert

   smFP _Myc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHPRT-DRGFP

   Plasmid

   #26476

   Purpose

   DR-GFP substrate (Addgene 26475) flanked by mouse hprt targeting sequences

   Depositor

   Maria Jasin

   Article

   Pierce et al Genes Dev. 2001 Dec 15. 15(24):3237-42.

   Insert

   pHPRT-DRGFP
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28b_RanT24N

   Plasmid

   #186271

   Purpose

   Expression vector of His-RanT24N in E.coli

   Depositor

   Masahito Ikawa

   Article

   Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

   Insert

   GTP-binding nuclear protein Ran (Ran Mouse)
   

   Use

   Tags

   His tagged and His-tagged at N terminal

   Expression

   Bacterial

   Mutation

   Changed T24 into N

   Promoter

   T7 promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pWPXL

   Plasmid

   #12257

   Purpose

   2nd generation lentiviral transfer plasmid

   Depositor

   Didier Trono

   Type

   Empty backbone

   Use

   Cre/Lox and Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-NGFP-VMAn-FKBP

   Plasmid

   #70225

   Purpose

   Expresses N-terminal portion of GFP along with VMA intein for protein splicing

   Depositor

   Perry Howard

   Article

   Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

   Insert

   eGFP
   

   Use

   Tags

   FK506- and rapamycin-binding protein (FKBP), Link…

   Expression

   Mammalian

   Mutation

   Changed Glu 125 to Ile, Deleted amino acids 129-2…

   Promoter

   CMV

   Available since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3-FRB-VMAc-CGFP

   Plasmid

   #70226

   Purpose

   Expresses C-terminal portion of GFP along with VMA intein for protein splicing

   Depositor

   Perry Howard

   Article

   Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

   Insert

   eGFP
   

   Use

   Tags

   FKBP-rapamycin binding (FRB) domain of the mammal…

   Expression

   Mammalian

   Mutation

   Changed Ile 129 to Cys, Deleted amino acids 1-128

   Promoter

   CMV

   Available since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pLVX TRE 3G BFP

   Plasmid

   #128070

   Purpose

   Lentiviral expression of Tet inducible TagBFP

   Depositor

   Oskar Laur

   Insert

   TagBFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   no

   Promoter

   Available since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pmGFP-P2A-K0-P2A-RFP

   Plasmid

   #105686

   Purpose

   Double fluorescent stalling reporter K0

   Depositor

   Ramanujan Hegde

   Article

   Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

   Inserts

   P2A-3xFLAG-VHPbeta
   K0
   P2A-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (from vector)

   Available since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pANCH2-Nat

   Plasmid

   #87249

   Purpose

   gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT)

   Depositor

   Kerstin Bystricky

   Article

   Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

   Insert

   Nat
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-CCND1

    Plasmid

    #172632

    Purpose

    Expresses untagged cyclin D1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge