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pPB.DEST-Illusia(YYFF)

Plasmid

#215445

Purpose

Piggybac expression vector with inactive phospho-mutant (YYFF) of Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia(YYFF)

Use

Transposon-based stable expression

Expression

Mammalian

Promoter

CAG

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pHR-Flag-IF1-Fibcon-icam

Plasmid

#233076

Purpose

expresses synCAM -IF1-fibcon - iCAM1

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

If1-Icam

Use

Lentiviral

Expression

Mammalian

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-dCas9-VP64

Plasmid

#235595

Purpose

Expresses the dCas9-VP64 under the control of human EF1a promoter

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

dCas9-VP64

Use

CRISPR

Tags

NLS

Expression

Mammalian

Promoter

EF1a

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 JDM16-GFPnb-mCh

Plasmid

#235667

Purpose

Expresses designed inhibitor of Hsp70 tagged with a GFP nano body and mCherry

Depositor

David Baker

Article

Zhang et al Cell Chem Biol. 2025 Feb 4:S2451-9456(25

Insert

JDM16-GFPnb-mCh (Mch Synthetic)

Tags

GFP nanobody and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX302_circSMAD2_OE

Plasmid

#215238

Purpose

Overexpression of pLEX302_circSMAD2(2,7)_OE expression

Depositor

Sefi Rosenbluh

Article

Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

Insert

circSMAD2

Use

Lentiviral

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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shRNA_LacZ

Plasmid

#215217

Purpose

Supression of shLacZ expression (Negative control)

Depositor

Sefi Rosenbluh

Article

Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

Insert

LacZ shRNA

Use

Lentiviral

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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plko_TRE3G_Myc-Sox9WT_PGKpuroR

Plasmid

#203962

Purpose

Dox inducible WT SOX9 with myc tag

Depositor

Elaine Fuchs

Article

Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

Insert

SOX9 (Sox9 Mouse)

Use

Lentiviral

Tags

myc

Promoter

TRE3G

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAS_pb_Ef1a-TIR1_Ubc-mCherry-Puro

Plasmid

#195567

Purpose

PiggyBac plasmid for stable expression of TIR1, mCherry, and Puromycin resistance in mammalian cells

Depositor

Joanna Wysocka

Article

Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

Insert

TIR1

Expression

Mammalian

Promoter

Ef1-alpha

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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p38.pA.OFP.mCMV.hPGK.G5-Flag-NGFR.WPRE

Plasmid

#226761

Purpose

5G repeat, NGFR in p20

Depositor

Ferdinando Pucci

Article

Hamilton et al Adv Biosyst. 2020 Dec;4(12):e2000007. do

Insert

ngfr (NGFR Human)

Expression

Mammalian

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-Tir

Plasmid

#221326

Purpose

Dox-inducible expression of Tir in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

Tir

Use

Retroviral

Tags

HN-FLAG

Promoter

TRE3GV

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-Gag

Plasmid

#221325

Purpose

Dox-inducible expression of Gag in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

Gag

Use

Retroviral

Tags

FLAG

Promoter

TRE3GV

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS

Plasmid

#217478

Purpose

Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1s

Depositor

Peng Zou

Article

Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

Insert

Nier1s

Tags

3xNLS, P2A-EGFP-3xNLS

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS

Plasmid

#217479

Purpose

Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1b

Depositor

Peng Zou

Article

Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

Insert

Nier1b

Tags

3xNLS, P2A-EGFP-3xNLS

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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MCP-YPet

Plasmid

#215747

Purpose

Expresses MS2 coat protein (MCP) fused to YPet

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

YPet

Expression

Mammalian

Promoter

EF1a

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMAT_41BBICD

Plasmid

#197097

Purpose

This plasmid contains the coding sequence for the intracellular domain of 4-1BB. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

Depositor

Wendell Lim

Article

Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

Insert

This plasmid contains the coding sequence for the intracellular domain of 4-1BB.

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMAT_CD28ICD

Plasmid

#197098

Purpose

This plasmid contains the coding sequence for the intracellular domain of CD28. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

Depositor

Wendell Lim

Article

Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

Insert

This plasmid contains the coding sequence for the intracellular domain of CD28.

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST14-Cys-SpyCatcher (Min)

Plasmid

#216713

Purpose

Expresses truncated minimal form of SpyCatcher with free cysteine for labeling in bacteria. See also Li...Howarth, J Mol. Biol., 2014

Depositor

Alex Dunn

Article

Zhong et al Cell Rep Methods. 2022 Sep 2;2(9):100278

Insert

Cys-SpyCatcher (Min)

Tags

6xHis

Expression

Bacterial

Mutation

See Li...Howarth, J. Mol. Biol., 2014

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1alpha-NlaCas7-p65

Plasmid

#207394

Purpose

Expresses NlaCas7-p65 activator fusion in mammalian cells

Depositor

Yan Zhang

Article

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-NlaCas7-p65-bGH polyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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3'-BirA*-FLAG minimal CMV pCDNA5

Plasmid

#212047

Purpose

LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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5'-BirA*-FLAG minimal CMV pCDNA5

Plasmid

#212046

Purpose

LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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