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pcDNA3.1(+) Drp1 V2

Plasmid

#44599

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

Drp1 variant 2 (Dnm1l Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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LAMP-CeNL_pcDNA3

Plasmid

#89514

Purpose

Cyan color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Use

Tags

LAMP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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LAMP-ReNL_pcDNA3

Plasmid

#89532

Purpose

Red color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Use

Tags

LAMP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hNoxa1

Plasmid

#58531

Purpose

expresses human Noxa1 in mammalian cells

Depositor

Botond Banfi, Karl-Heinz Krause

Article

Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. E

Insert

NADPH oxidase activator 1 (NOXA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hNoxo1

Plasmid

#58530

Purpose

expresses human Noxo1 in mammalian cells

Depositor

Botond Banfi, Karl-Heinz Krause

Article

Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. E

Insert

NADPH oxidase organizer 1 (NOXO1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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LAMP-OeNL_pcDNA3

Plasmid

#89525

Purpose

Orange color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Use

Tags

LAMP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mNoxa1

Plasmid

#58339

Purpose

expresses mouse Noxa1 in mammalian cells

Depositor

Botond Banfi, Karl-Heinz Krause

Article

Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. E

Insert

NADPH oxidase activator 1 (Noxa1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRIPK1-Flag-S320A

Plasmid

#112491

Purpose

Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

Depositor

Junying Yuan

Insert

RIPK1 (RIPK1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S320A

Promoter

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRIPK1-Flag-K377R

Plasmid

#112489

Purpose

Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

Depositor

Junying Yuan

Insert

RIPK1 (RIPK1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

K377R

Promoter

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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cRel RHD cFlag pcDNA3

Plasmid

#20016

Depositor

Stephen Smale

Insert

c-Rel (Rel Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Contains only the Rel Homology Domain of c-Rel. I…

Promoter

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-hRIPK1-Flag-K599R

Plasmid

#112490

Purpose

Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

Depositor

Junying Yuan

Insert

RIPK1 (RIPK1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

K599R

Promoter

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRIPK1-Flag-K115R

Plasmid

#112488

Purpose

Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

Depositor

Junying Yuan

Insert

RIPK1 (RIPK1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

K115R

Promoter

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HshnRNPE1_163-278_Z

Plasmid

#148116

Purpose

Mammalian Expression of HshnRNPE1_163-278

Depositor

Elisa Izaurralde

Insert

HshnRNPE1_163-278 (PCBP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-dPlcR(K30/32L)

Plasmid

#181833

Purpose

Phospholipid binding-deficient Lyn-dPlcR negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-dPlcR (K30L,K32L)

Use

Tags

N-terminal targeting sequence from Lyn kinase, dd…

Expression

Mammalian

Mutation

Lysines 30 and 32 in PH domain mutated to leucine.

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-PlcR(K30/32L)

Plasmid

#181831

Purpose

Phospholipid binding-deficient Lyn-PlcR negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR (K30L,K32L)

Use

Tags

ECFP, EYFP, and N-terminal targeting sequence fro…

Expression

Mammalian

Mutation

Lysines 30 and 32 in PH domain mutated to leucine.

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MCS-BirA(R118G)-HA-MACROD1

Plasmid

#172581

Purpose

For transient expression of C-terminal BirA-tagged MACROD1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Use

Tags

BirA(R118G)-HA

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-OTUB1 T134R,C91S-pcDNA3.1

Plasmid

#118212

Purpose

Expresses FLAG-OTUB1 T134R,C91S in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

changed cysteine 91 to serine and threonine 134 t…

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(H56A, R68A, R97A, K98A)

Plasmid

#231379

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Article

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Human, Synthetic)

Use

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

H56A, R68A, R97A, K98A

Promoter

CMV

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hyg-BFP-P2A-ppViperin

Plasmid

#217481

Purpose

Expression of Blue Fluorescent Protein - P2A - full length protein kinase R from chinook salmon

Depositor

Bertrand Collet

Article

Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

Insert

BFP-P2A-otPKRFL

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MCS-BirA(R118G)-HA-MACROD2

Plasmid

#172582

Purpose

For transient expression of C-terminal BirA-tagged MACROD2

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD2 (MACROD2 Human)

Use

Tags

BirA(R118G)-HA

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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