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Showing: 1021 - 1040 of 19147 results

  4. RIP7-RLuc-YFP-pBS

    Plasmid
    #72482
    Purpose
    Expresses a renilla luciferase-YFP fusion protein under control of the rat insulin 2 gene promoter (RIP7) suitable for transfection or generation of transgenic animals
    Depositor
    Vincent Poitout
    Article
    Ghislain et al Endocrinology. 2015 Dec 15:en20151550.
    Inserts
    rat ins2 gene promoter (Ins2 Rat)
    luciferase
    eYFP
    Use
    Luciferase
    Tags
    eYFP and renilla luciferase
    Expression
    Mammalian
    Mutation
    eYFP varient
    Promoter
    Available since
    March 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    72482-plasmid-map-sequence-id-136970

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  12. PX458-gR2A_ISO

    Plasmid
    #124808
    Purpose
    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a> plasmids to convert expression of rat IgG2a hybridomas to isotype of choice.
    Depositor
    Martijn Verdoes, Ferenc Scheeren
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    hSPCas9
    Use
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124808-plasmid-map-sequence-id-244309

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  17. pHybr_r2a>mG2b-srt-his

    Plasmid
    #124804
    Purpose
    HDR template to knock-in mouse IgG2b Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2b producing cell lines.
    Depositor
    Martijn Verdoes, Ferenc Scheeren
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgG2b
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124804-plasmid-map-sequence-id-244314

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  18. pHybr_r2a>mG2a-srt-his

    Plasmid
    #124803
    Purpose
    HDR template to knock-in mouse IgG2a Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2a producing cell lines.
    Depositor
    Martijn Verdoes, Ferenc Scheeren
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgG2a
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124803-plasmid-map-sequence-id-244318

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  19. pHybr_r2a>m2a(silent)-srt-his

    Plasmid
    #124807
    Purpose
    HDR template to knock-in FC silent mIgG2a isotype in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to FC silent producing cell lines.
    Depositor
    Martijn Verdoes, Ferenc Scheeren
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgG2a (Fc silent)
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Mutation
    L234A/L235A/N297A
    Promoter
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124807-plasmid-map-sequence-id-251832

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  20. PX459-gR2A_Hinge

    Plasmid
    #124811
    Purpose
    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.
    Depositor
    Martijn Verdoes, Ferenc Scheeren
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Cas9 – gRNA_R2A_hinge
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Bacterial and Mammalian
    Mutation
    R166H in PuroR (please see depositors comment bel…
    Promoter
    pUC
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124811-plasmid-map-sequence-id-243700

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Showing: 1021 - 1040 of 19147 results