We narrowed to 7,221 results for: ALP

1. pLenti-EF1alpha-IRES-EGFP-3XFlag-hCEP164

   Plasmid

   #122966

   Purpose

   Expresses 3XFlag-tagged human CEP164 in mammalian cells

   Depositor

   FENG-QIAN Li, Ken-Ichi Takemaru

   Insert

   CEP164 (CEP164 Human)
   

   Use

   Lentiviral

   Tags

   3XFlag

   Expression

   Mutation

   Promoter

   EF1 alpha

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PGC-1 alpha promoter luciferase delta MEF

   Plasmid

   #8889

   Depositor

   Bruce Spiegelman

   Article

   Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

   Insert

   PGC-1 alpha promoter dMEF (Ppargc1a Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Site-directed mutagenesis to remove the MEF2 bind…

   Promoter

   Available since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-FLEX-PKIalpha-IRES-nls-mRuby2

   Plasmid

   #63059

   Purpose

   Expresses PKIalpha and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

   Depositor

   Bernardo Sabatini

   Article

   Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

   Insert

   PKIalpha (Pkia Synthetic, Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-CMV-Galpha-i3-IRESWT-Gbetagamma ONE-GO

   Plasmid

   #204341

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   Galpha-i3/Gbetagamma ONE-GO
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. hum alpha ENAC promoter a25-a23

   Plasmid

   #83427

   Purpose

   promoter reporter construct to test 5' flanking sequences

   Depositor

   Christie Thomas

   Article

   Sayegh et al J Biol Chem. 1999 Apr 30;274(18):12431-7

   Insert

   human aENaC gene 5' flanking seq + 55 nt of the 5' UTR for exon 1A (SCNN1A Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mutation

   Promoter

   alpha ENAC

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDGB3_alpha1-pAtUBQ10:HA-LRR-NEL-mcherry-Tnos

   Plasmid

   #211840

   Purpose

   Plant transient expression cassette for the catalytically inactive E3-DART ligase under a UBQ10 promoter.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   LRR-NEL
   

   Use

   Synthetic Biology

   Tags

   HA and mCherry

   Expression

   Plant

   Mutation

   Promoter

   UBIQUITIN10

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. V309 - pDNR MCS SA lacZ alpha - Donor

   Plasmid

   #11692

   Depositor

   Tony Pawson

   Article

   Colwill et al BMC Biotechnol. 2006 . 6():13.

   Type

   Empty backbone

   Use

   Cre/Lox; Donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pDGB3_alpha1-pAtUBQ10:HA-LRR-NELC492A-mcherry-Tnos

   Plasmid

   #211843

   Purpose

   Plant transient expression cassette for the E3-DART ligase under a UBQ10 promoter.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   LRR-NEL
   

   Use

   Synthetic Biology

   Tags

   HA and mCherry

   Expression

   Plant

   Mutation

   C492A

   Promoter

   UBIQUITIN10

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-hSyn-Galpha-i3-IRESWT-Gbetagamma ONE-GO

   Plasmid

   #204343

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   hSyn Galpha-i3/Gbetagamma ONE-GO
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGB3_alpha1-AtUBQ10:HR1bR181/185A-GFP-T35S

    Plasmid

    #211839

    Purpose

    Plant transient expression cassette for GFP fused to the mutant HR1b degron sequence under UBQ10 promoter.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    HR1b
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Plant

    Mutation

    R181A R185A

    Promoter

    UBIQUITIN10

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Stat1 alpha Y701F Flag pRc/CMV

    Plasmid

    #8702

    Depositor

    Jim Darnell

    Article

    Wen et al Cell 1995 Jul 28;82(2):241-50.

    Insert

    STAT1 alpha Y701F (STAT1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Y701F

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pAAV MP-135 mRFP-3MREs (AAV-alpha)

    Plasmid

    #206929

    Purpose

    Alpha cell-specific gene expression from AAV in human pancreatic islets

    Depositor

    Markus Grompe

    Insert

    mRFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MP-135

    Available since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

    Plasmid

    #128749

    Purpose

    Expresses control gRNA

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PBS/pU6- HIF1 alpha RNAi plasmid 1

    Plasmid

    #21103

    Depositor

    Connie Cepko

    Article

    Chen et al Science. 2009 Jan 9. 323(5911):256-9.

    Insert

    RNAi against hypoxia inducible factor 1alpha
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-EF1alpha-VP64-dCas9-VP64-ZsG

    Plasmid

    #192667

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A ZsGreen1

    Depositor

    Jeffrey Miner

    Insert

    VP64-dCas9-VP64, ZsGreen1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Danio alpha A-crystallin 1kb promoter/AcGFP

    Plasmid

    #98096

    Purpose

    aA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

    Depositor

    Mason Posner

    Article

    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

    Insert

    Alpha A-crystallin promoter 1 kb fragment, Danio rerio (cryaa Zebrafish)
    

    Use

    Gfp expressing

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Danio alpha A-crystallin 1 kb fragment (-1028/-1)

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-hSyn-Galpha-s-IRESWT-Gbetagamma ONE-GO

    Plasmid

    #204342

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    hSyn Galpha-s/Gbetagamma ONE-GO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGB3 alpha1 P35S:PhiC31 integrase-RDF:T35S (GB2893)

    Plasmid

    #160633

    Purpose

    TU for the constitutive expression of the PhiC31 integrase, C-terminally fused with its reversion factor (RDF)

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31-RDF
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-mNG-eDHFR(69K6)-G-alpha-q(L254A)

    Plasmid

    #178851

    Purpose

    Mammalian expression of Gαq(L254A) fused to mNeonGreen-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    mNeonGreen-eDHFR(69K6)-Gαq(L254A) (GNAQ Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Gαq: C9S/C10S/Q209L/L254A

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-EF1alpha-scFv-p65-HSF1-Blast

    Plasmid

    #192652

    Purpose

    3rd generation lenti vector encoding scFV-p65-HSF1 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    scFv-p65-HSF1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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