We narrowed to 2,467 results for: PUC19

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qTAG-AAVS1-PGK-Zeo

Plasmid

#227271

Purpose

Empty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-Ef1a-Zeo

Plasmid

#227268

Purpose

Empty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mScarlet-EFS-Blast

Plasmid

#227260

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAP758-1

Plasmid

#99494

Purpose

TEV::eGFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::3XFlag

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGGE009

Plasmid

#48841

Purpose

Provides the UBQ10 terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

UBQ10 terminator

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGF003

Plasmid

#48844

Purpose

Provides a D-alanine resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pMAS::D-AlanineR::tMAS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCfB8792

Plasmid

#126910

Purpose

Template for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 gene

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

ADE2 gRNA

Use

Synthetic Biology

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pUPRT::Floxed DHFR-TS*

Plasmid

#100606

Purpose

TgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.

Depositor

David Sibley

Insert

TgUPRT::Floxed TgDHFR-TS*

Use

Toxoplasma expression

Mutation

Pyrimethamine resistance

Promoter

pTgDHFR-TS

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEA038

Plasmid

#224547

Purpose

Blast-T2A-2xHA-FKPB12(dTagDegron) mouse Nipbl N-term targeting vector

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

FKBP12F36V degron (dTAG system), blasticidin, 2xHA tag

Use

Mouse Targeting

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pADH118-2

Plasmid

#90984

Purpose

NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Insert

NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. albicans LEU2 2 of 2

Use

CRISPR

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pTW072M

Plasmid

#115960

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl2 (nsP2 Q739L)

Use

Synthetic Biology

Mutation

nsP2 Q739L

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pEF1α-Cre-2A-miRFP670nano3

Plasmid

#207747

Purpose

Expression of the Cre recombinase linked to a miRFP670nano3 fluorescent marker

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

Cre-PGK-miRFP670nano3

Use

Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-Cas12i2-T2A-EGFP

Plasmid

#188270

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

Cas12i2

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTetQCas-8+IS186

Plasmid

#170636

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(8+IS186 array)

Expression

Bacterial

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRepair-MPP8_mAID-P2A-BFP

Plasmid

#175036

Purpose

Homology Directed Repair construct for C-terminal tagging of mMPP8 with mAID using CRISPR/Cas9

Depositor

Kristian Helin

Article

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

mAID-P2A-BFP

Use

Hdr donor template

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pSL0715 (pDonor_R1-57)

Plasmid

#130644

Purpose

Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened right end. Total transposon size = 910 bp.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCAST donor DNA (short R)

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pICH82094

Plasmid

#48074

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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PB-TRE-GNL

Plasmid

#204499

Purpose

Expresses Glis1, Nanog and lin28a by tet-on promoter in mammalian cells

Depositor

Wataru Fujii

Article

Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

Insert

Glis1 (Glis1 Mouse)

Expression

Mammalian

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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ATP1A1_T804N_hPGK1_mScarlet-I_Donor

Plasmid

#173207

Purpose

HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I cassette to ATP1A1 intron 17.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1-T804N mScarlet-I donor

Use

CRISPR

Expression

Mammalian

Promoter

hPGK1

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pPN217

Plasmid

#91676

Purpose

Express sgRNA targeting human TLE1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

TLE1 (TLE1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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