We narrowed to 8,588 results for: Set

1. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-A10

   Plasmid

   #196083

   Purpose

   Inducible CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Insert

   gRNA targeting Adam10
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Cloned using SapI sites - destroyed after inserti…

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-A10

   Plasmid

   #196075

   Purpose

   Constitutive CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Insert

   gRNA targeting Adam10
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Cloned using SapI sites - destroyed after inserti…

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-A10

   Plasmid

   #196077

   Purpose

   Constitutive CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Insert

   gRNA targeting Adam10
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Cloned using SapI sites - destroyed after inserti…

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENT-L3-pSnail-L5

   Plasmid

   #186365

   Purpose

   Entry clone with ORF encoding Ci-Snail cis-regulatory region flanked by Gateway recombination sequences

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   Snail regulatory sequence
   

   Use

   Protein expression

   Promoter

   T7 promoter

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-pSP172BSSPE-Swa1::HA-RfA

   Plasmid

   #186404

   Purpose

   Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Type

   Empty backbone

   Use

   Gateway destination vector

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. E42_control_NGFR

   Plasmid

   #189800

   Purpose

   Retroviral delivery of control guide RNA

   Depositor

   Ellen Rothenberg

   Article

   Hosokawa et al Nat Immunol. 2018 Dec;19(12):1427-1440.

   Insert

   Luciferase gRNA
   

   Use

   Retroviral

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENT-R4-bpFOG-L5

   Plasmid

   #186369

   Purpose

   Entry clone with ORF encoding Strong basal promoter of FOG flanked by Gateway recombination sequences

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   Basal promoter of the Friend Of Gata gene
   

   Use

   Protein expression

   Promoter

   T7 promoter

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENT-L3-pFOGl-L5

   Plasmid

   #186364

   Purpose

   Entry clone with ORF encoding Ci-FOG cis-regulatory region flanked by Gateway recombination sequences

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   Friend Of Gata regulatory sequence
   

   Use

   Protein expression

   Promoter

   T7 promoter

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA

   Plasmid

   #186407

   Purpose

   Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined gene of interest under control of an AttB3/B5 recombined reg. sequence.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Type

   Empty backbone

   Use

   Gateway destination vector

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PDEST-pSP172BSSPE-pFOGc::RfA-ECFP

    Plasmid

    #186403

    Purpose

    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter ECFP under control of the pFOG ectodermal regulatory sequence.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDEST-pSP172BSSPE-Swa1::RfA-ECFP

    Plasmid

    #186402

    Purpose

    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter eCFP under control of a reg. sequence in the Swa1 restriction site.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDEST-pSP172BSSPE-pFOGc::RfA-venus

    Plasmid

    #186401

    Purpose

    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter Venus under control of the pFOG ectodermal regulatory sequence.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDEST-pSP172BSSPE-pFOGc::venus-RfA

    Plasmid

    #186400

    Purpose

    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of the pFOG ectodermal regulatory sequence.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST-pSP172BSSPE-Swa1::venus-RfA

    Plasmid

    #186398

    Purpose

    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of a reg. sequence in the Swa1 restriction site.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENT-L3-a-element-L4

    Plasmid

    #186366

    Purpose

    Entry clone with ORF encoding Otx cis-regulatory region flanked by Gateway recombination sequences

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    a-element early Otx neural enhancer
    

    Use

    Protein expression

    Promoter

    T7 promoter

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST-pSP172BSSPE-Swa1::RfA-HA

    Plasmid

    #186405

    Purpose

    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of a reg. sequence in the Swa1 restriction site.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PDEST-pSP172BSSPE-pFOGc::RfA-HA

    Plasmid

    #186406

    Purpose

    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of the pFOG ectodermal regulatory sequence.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR221 CARD8 FIIND

    Plasmid

    #169963

    Purpose

    pDONR221 for Gateway shuttling. Contains the FIIND (L162M-P434, no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTEI037

    Plasmid

    #105413

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    Hormone-binding domain (glucocorticoid receptor) fused to mEGFP
    

    Use

    Synthetic Biology

    Mutation

    BsaI/ BpiI restriction sites removed

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTEI041

    Plasmid

    #105414

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    Hormone-binding domain and regulatory sequences (estrogen receptor)
    

    Use

    Synthetic Biology

    Mutation

    BsaI/ BpiI restriction sites removed

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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