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SPDK3889(TRV-RNA2-pPEBV-MCS-AtMet-tRNA)

Plasmid

#149277

Purpose

Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

Depositor

Savithramma Dinesh-Kumar

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

Modified TRV RNA2 with PEBV promoter and AtMet-tRNA mobile RNA sequence

Use

T-dna vector

Mutation

T2318C (DNA) in PEBV coat protein sequence

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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sgLenti-orthogonal

Plasmid

#105997

Purpose

Dual Streptococcus pyogenes and Staphylococcus aureus sgRNA expression vector

Depositor

Mike McManus

Article

Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

Type

Empty backbone

Use

CRISPR and Lentiviral; S.pyogenes sgrna expressio…

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGG-NFIX2

Plasmid

#112699

Purpose

Expression of NFIX2 protein and GFP

Depositor

Richard Gronostajski, Francois Guillemot

Article

Martynoga et al Genes Dev. 2013 Aug 15;27(16):1769-86. d

Insert

Nuclear Factor I X2 (Nfix Mouse)

Tags

HA

Expression

Mammalian

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLN540 (FuGW-G8p-IL12-P2A-CCL21-1Pe)

Plasmid

#105201

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 and CCL21 transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-IL12-P2A-CCL21-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 21, 2018

Availability

Academic Institutions and Nonprofits only

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pMB1-A

Plasmid

#190114

Purpose

Level 1 MoClo plasmid for A. baumannii

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPT24

Plasmid

#184419

Purpose

Transformation of mitochondrial DNA in yeast

Depositor

Martin Ott

Article

Rzepka et al STAR Protoc. 2022 May 25;3(2):101359. do

Type

Empty backbone

Expression

Yeast

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pGL2314

Plasmid

#137054

Purpose

loxP (empty) expression cassette: c-terminal 6xHA

Depositor

Jeremy Mottram

Article

Duncan et al Methods Mol Biol. 2019;1971:211-224. doi

Type

Empty backbone

Use

Leishmania floxed gene expression

Tags

6xHA

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pJOE6261.2

Plasmid

#135079

Purpose

P. putida KT2440 rescue construct

Depositor

Josef Altenbuchner

Article

Graf et al Appl Environ Microbiol. 2011 Aug;77(15):

Insert

upp (upp Pseudomonas putida)

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-C9orf114-gfp

Plasmid

#70266

Purpose

Expresses C9orf114 in mammalian cells with GFP reporter.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

C9orf114 (SPOUT1 )

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Nov. 9, 2015

Availability

Academic Institutions and Nonprofits only
pMXs-C3orf17-gfp

Plasmid

#70265

Purpose

Expresses C3orf17 in mammalian cells with GFP reporter.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

C3orf17 (NEPRO Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Nov. 9, 2015

Availability

Academic Institutions and Nonprofits only
pT-TK

Plasmid

#31549

Depositor

Yegor Vassetzky

Article

Dmitriev et al BMC Res Notes. 2008 Dec 23;1:135. doi: 1

Insert

LoxP

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pENTR-L5-EGFP-L2

Plasmid

#32593

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

EGFP (eGFP )

Use

Gateway Cloning

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pENTR-L5-LucmiR-EGFP-L2

Plasmid

#32587

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

Intronic Luciferase miRNA and EGFP

Use

Gateway Cloning

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-SYN-R5

Plasmid

#32582

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

Synapsin I promoter

Use

Gateway Cloning

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-CAMKII(0.4)-L4

Plasmid

#32579

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

CAMKIIalpha promoter 0.4kb version

Use

Gateway Cloning

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pINS-Neo

Plasmid

#31390

Depositor

Yegor Vassetzky

Article

Dmitriev et al BMC Res Notes. 2008 Dec 23;1:135. doi: 1

Insert

aph

Use

Cre/Lox

Available Since

April 10, 2012

Availability

Academic Institutions and Nonprofits only

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pGPS21loxFRTNeo

Plasmid

#27180

Depositor

Yoh Wada

Article

Aoyama et al Nucleic Acids Res. 2005 . 33(5):e52.

Insert

lox/Frt/-PGK/EM7pm-G418/Kan-lox/Frt cassette flanked by Tn7L/R

Use

In vitro transposion

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pEBB-II-I-deltaNLS1-EGFP

Plasmid

#22150

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of amino acids 297-304

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pBS-M71-IRES-tauRFP2ACNf

Plasmid

#15510

Depositor

Peter Mombaerts

Article

Li et al Nature. 2004 Mar 25. 428(6981):393-9.

Insert

M71 targeting vector with IRES tauRFP-RFP ACN

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MKK7g1(K165A)

Plasmid

#14675

Depositor

Roger Davis

Article

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Insert

MKK7g1(K165A) (Map2k7 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Lys-165Ala

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only