We narrowed to 10,989 results for: T7

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pET303C-hSOD1 A4V/C6S

Plasmid

#139667

Purpose

Expression plasmids containing human A4V/C6S SOD1

Depositor

Svetlana Antonyuk

Article

Chantadul et al Commun Biol. 2020 Mar 5;3(1):97. doi: 10

Insert

Superoxide dismutase-1 (SOD1 Human)

Expression

Bacterial

Mutation

change alanine 4 to valine, change cysteine 6 to …

Promoter

T7

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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His-MBP-CHMP1B, S2C

Plasmid

#184803

Purpose

Bacterial expression of CHMP1B (S2C mutation)

Depositor

James Hurley

Article

Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

CHMP1B (CHMP1B Human)

Tags

His6-MBP

Expression

Bacterial

Mutation

S2C

Promoter

T7

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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His6-EGFP-cPHx1

Plasmid

#183674

Purpose

PtdIns(3,4)P2 biosensor for purification from bacteria

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

PLEKHA1 (PLEKHA1 Human)

Tags

His6-EGFP

Expression

Bacterial

Mutation

Amino acids 169-329

Promoter

T7

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pET-SU(Z)12(624-900)-H6

Plasmid

#183772

Purpose

Express of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.

Depositor

Peter Harte

Article

Tie et al Mol Cell Biol. 2003 May;23(9):3352-62. d

Insert

SU(Z)12 residues 624-900 (Su(z)12 Fly)

Tags

His tag at the C-terminus

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-GST-BTLAicd (AA190-289, Y257F, Y282F)-TwinStrep

Plasmid

#180811

Purpose

Expression of GST fused human BTLA intracellular domain (AA190-289, Y257F, Y282F)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

GST-BTLAint (AA190-289, Y257F, Y282F)-TwinStrep (BTLA Human)

Expression

Bacterial

Mutation

BTLA (Y257F, Y282F)

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-His10-BTLAicd (AA190-289)-TwinStrep

Plasmid

#180805

Purpose

Expression of 10*His tagged human BTLA intracellular domain (AA190-289)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLAint (AA190-289)-TwinStrep (BTLA Human)

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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iDianiSnFR

Plasmid

#177741

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

iDianiSnFR

Tags

HA, His, and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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CEA-N domain residues 1-132 (CEACAM5)-pET30b

Plasmid

#174693

Purpose

Plasmid expressing CEA-N domain

Depositor

Jean Gariepy

Article

Abdul-Wahid et al Int J Cancer. 2016 Aug 15;139(4):841-53.

Insert

CEA-N Domain

Tags

TEV Cleavage Site and hexahistidine (HIS) tag

Expression

Bacterial

Mutation

none

Promoter

T7 Promoter

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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iCytSnFR

Plasmid

#177738

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

iCytSnFR

Tags

HA, His, and Myc

Expression

Bacterial

Promoter

T7

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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iCyt_BrEt_SnFR

Plasmid

#177740

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

iCyt_BrEt_SnFR

Tags

HA, His, and Myc

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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iCyt_F_SnFR

Plasmid

#177739

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

iCyt_F_SnFR

Tags

HA, His, and Myc

Expression

Bacterial

Promoter

T7

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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p36-p37-p38-p40-pET-Duet-1-Rfc3-T76D

Plasmid

#175051

Purpose

Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

Depositor

Brian Kelch

Article

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunit 5 (RFC2, RFC3, RFC4, RFC5 Human)

Expression

Bacterial

Mutation

changed Thr76 to Asp, phosphomimetic mutation

Promoter

T7 promoter

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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p36-p37-p38-p40-pET-Duet-1-Rfc3-3KtoA

Plasmid

#175050

Purpose

Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

Depositor

Brian Kelch

Article

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunits 2-5, Rfc3-3KtoA-mutation (RFC4, RFC2, RFC3, RFC5 Human)

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-dre4

Plasmid

#170440

Purpose

Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.

Depositor

Joan Conaway

Article

Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

Insert

dre4 (dre4 Fly)

Tags

6xHis and V5

Expression

Bacterial

Mutation

Deleted amino acids 1-20

Promoter

T7

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST17-cDAXX-0SB

Plasmid

#169730

Purpose

Bacterial expression of N-terminally 6His tagged cDAXX-0SB

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

cDAXX-0SB (DAXX Human)

Tags

6 His

Expression

Bacterial

Mutation

Comprises residues 495-741 and has the major SB m…

Promoter

T7

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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A1-LCD Aro--

Plasmid

#169716

Purpose

Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids.

Depositor

Tanja Mittag

Article

Martin et al Science. 2020 Feb 7;367(6478):694-699. d

Insert

hnRNPA1 (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

hnRNPA1-A Low complexity domain including amino a…

Promoter

T7

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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A1-LCD Perfect Mix

Plasmid

#169718

Purpose

Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids. TYR and PHE shuffled into 5 clusters.

Depositor

Tanja Mittag

Article

Martin et al Science. 2020 Feb 7;367(6478):694-699. d

Insert

hnRNPA1 (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

hnRNPA1-A Low complexity domain including amino a…

Promoter

T7

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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A1-LCD Aro+

Plasmid

#169717

Purpose

Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids. PHE and TYR amino acids shuffled to optimize mixing.

Depositor

Tanja Mittag

Article

Martin et al Science. 2020 Feb 7;367(6478):694-699. d

Insert

hnRNPA1 (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

hnRNPA1-A Low complexity domain including amino a…

Promoter

T7

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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A1-LCD Patchy

Plasmid

#169719

Purpose

Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids.

Depositor

Tanja Mittag

Article

Martin et al Science. 2020 Feb 7;367(6478):694-699. d

Insert

hnRNPA1 (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

hnRNPA1-A Low complexity domain including amino a…

Promoter

T7

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSMT3-FBF2 SS/Y

Plasmid

#166973

Purpose

Expresses FBF-2 with SS/Y mutations in repeat 5 in E. coli for purification and crystallization studies

Depositor

Traci Hall

Article

Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

Insert

FBF-2 (fbf-2 Nematode)

Tags

His-SUMO

Expression

Bacterial

Mutation

FBF-2 PUM RNA binding domain; amino acids 164-575…

Promoter

T7

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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