We narrowed to 23,259 results for: ESC

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VAMP2-pHuji

Plasmid

#107369

Purpose

Synaptic vesicle protein VAMP2 fused to the red FP pH sensor pHuji

Depositor

David Perrais

Article

Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

Insert

VAMP2 (Vamp2 Rat)

Tags

pHuji

Expression

Mammalian

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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shCDK4/6(2)

Plasmid

#73555

Purpose

vector encoding both shRNA against CDK4(2) and shRNA against CDK6(2)

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

shRNA against CDK4 + shRNA against CDK6

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
pCAG--uMASS_LF

Plasmid

#209763

Purpose

Expression of uMASS_LF (loss-of-function) in mammalian cells under CAG promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_LF

Expression

Mammalian

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-uMASS_1

Plasmid

#209762

Purpose

Expression of uMASS_1 in mammalian cells under CAG promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_1

Expression

Mammalian

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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ZJOM11

Plasmid

#133637

Purpose

Integration of GFP-TLG1 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

pCUP1-GFP-Tlg1 (TLG1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMX-hTAF10

Plasmid

#44340

Depositor

Marc Timmers

Article

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF10 (TAF10 Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only

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pUb-tdTomato-LP-Neo-LP

Plasmid

#231509

Purpose

Fluorescent reporter for tdTomato expression in Acanthamoeba via the Cre/loxP system

Depositor

Yeonchul Hong

Article

Aung et al Parasites Hosts Dis. 2025 Feb;63(1):25-3

Insert

tdTomato fluorescent protein

Use

Unspecified; Acanthamoeba castellanii neff strain

Promoter

Acanthamoeba polyubiquitin

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGAPDH-Cre-HYG

Plasmid

#231510

Purpose

Expresses Cre recombinase in Acanthamoeba

Depositor

Yeonchul Hong

Article

Aung et al Parasites Hosts Dis. 2025 Feb;63(1):25-3

Insert

Cre recombinase

Use

Unspecified; Acanthamoeba castellanii neff strain

Tags

EGFP and SV40 NLS

Promoter

Acanthamoeba GAPDH

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pKanCMV-CX4.3-7aa-mClover3

Plasmid

#74260

Purpose

Mammalian expression of C-terminal fusion of mClover3 to CX43

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

Connexin 43

Tags

mClover3

Expression

Mammalian

Promoter

CAG and CMV

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Caveolin-C-10

Plasmid

#55279

Purpose

Localization: Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Caveolin (CAV1 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mRuby2-PMP-N-10

Plasmid

#55910

Purpose

Localization: Peroxisomes, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

PMP (PXMP2 Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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CVS-N2c-tdTomato-FlpO

Plasmid

#172375

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

tdTomato + FlpO

Use

Neurotropic virus

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Poly(A)

Plasmid

#65524

Purpose

Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-GFP

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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AA302

Plasmid

#216052

Purpose

Fragmid fragment: (C' terminus) Nucleoplasmin NLS

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

NpNLS_v1.2; Stop; polyA_v1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEXL flag Smad3

Plasmid

#10920

Depositor

Bob Weinberg

Article

Liu et al Proc Natl Acad Sci U S A. 1997 Sep 30. 9

Insert

Smad3 (SMAD3 Human)

Tags

flag

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pKC158

Plasmid

#134242

Purpose

CENP-A Halo expression construct

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

CENP-A-Halo tagging construct (CENPA Human)

Use

Human targeting

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMV_AA090

Plasmid

#216099

Purpose

Knockout, EF1a-driven Cas12a (Cas only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas12a [EnAs]

Use

CRISPR and Lentiviral; Assembled vector

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pOBCol3.6-GFPtpz

Plasmid

#110211

Purpose

Labels immature osteoblasts and connective tissue cells expressing type 1 collagen.

Depositor

David Rowe

Article

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Insert

eGFPtopaz

Tags

None

Expression

Mammalian

Mutation

None

Promoter

3.6 kb rat type 1 collagen + first intron

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pGBW-m4136464

Plasmid

#149534

Purpose

Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDR111_Bgal NLS

Plasmid

#188396

Purpose

Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Insert

lacZ

Tags

PhoD secretion peptide and SV40 NLS

Expression

Bacterial

Promoter

Phyper spank

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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