We narrowed to 14,497 results for: SHR;

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Tet-myr-GFP

Plasmid

#83468

Purpose

Lentiviral vector for doxycycline-inducible expression of membrane targeted-GFP in mammalian cells

Depositor

Barry Gumbiner

Article

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

Myristoylated GFP

Use

Lentiviral

Expression

Mammalian

Promoter

doxycycline-inducible promoter

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - CTRL sgRNA

Plasmid

#70662

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a non-targeting sgRNA element from U6 promoter. Lentiviral backbone

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

non-targeting sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pBzCas13b-gRNA-1

Plasmid

#164858

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-1

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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p140Tol2-Olactb:SABER

Plasmid

#226829

Purpose

Expression of beta actin driven BFP and molecular barcode array for SABER-seq in zebrafish. Also contains heat-shock activated mCherry reporter and CreERT2.

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

BFP-SABER
mCherry-t2A-CreERT2

Use

Zebrafish expression

Promoter

Olactb and hsp70

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLVPT-rtTR-KRAB

Plasmid

#11777

Purpose

Tet-regulated (Tet-off) lentiviral vector for transgene (mPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GFP, rtTR-KRAB, Tet-off

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pU6a:sgRNA(tyr)

Plasmid

#64250

Purpose

expresses sgRNA(tyr) under U6a promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Insert

U6a:sgRNA (tyr)

Use

CRISPR

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgATG7

Plasmid

#211534

Purpose

Deletes ATG7

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgATG7

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pEX-A-U6-gRNA

Plasmid

#65626

Purpose

Empty vector for the expression U6 driven gRNA

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

U6-gRNA

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pEX128·gRNA

Plasmid

#187600

Purpose

Template for amplification of gRNA with Cas6 recognition site

Depositor

Pablo Ivan Nikel

Article

Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

Insert

gRNA template

Promoter

no promoter

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pM370

Plasmid

#173178

Purpose

(Empty Backbone) gRNA-12xMBS

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

gRNA-12xMBS

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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