We narrowed to 14,749 results for: EGF

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pCellFree_G03 USF2

Plasmid

#67069

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

USF2 (USF2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-LAMP1-mCherry

Plasmid

#214274

Purpose

Mammalian expression of LAMP1-mCherry (lysosome marker)

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

LAMP1-mCherry (Lamp1 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-ABE8e-V106W

Plasmid

#193646

Purpose

Expression of base editor dCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-TadA*8e-V106W, EGFP

Expression

Mammalian

Mutation

TadA*8e-V106W

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-LifeAct-GFP

Plasmid

#190199

Purpose

Expresses the small actin binding peptide, LifeAct, with a GFP reporter

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

LifeAct-eGFP

Use

AAV

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSin-GFP-Fg

Plasmid

#174307

Purpose

Lentiviral expression of GFP

Depositor

Bruce Beutler

Article

Yue et al Science. 2021 May 14;372(6543). pii: 372

Insert

GFP (eGFP Aequorea victoria)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-RnBarr2

Plasmid

#137800

Purpose

Visualization of beta-arrestin2

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin2 (Arrb2 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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VI_pAAV-ProA7-GFP-WPRE

Plasmid

#125891

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

GFP

Use

AAV

Promoter

ProA7

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pRS413-GAL1-luc*(-SKL)

Plasmid

#40234

Depositor

Michael Benton

Insert

luc*(-SKL)

Expression

Bacterial

Promoter

GAL1

Available Since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pPL001

Plasmid

#184750

Purpose

Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD.

Depositor

Jef Boeke

Article

Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

Insert

pEF1a-eGFP-T2A-BSD-bGHpA

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoG5

Plasmid

#193807

Purpose

CMV overexpression of ChemoG5 in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG5

Tags

10xHis

Expression

Mammalian

Mutation

EGFP:A206K, T225R; HT7: E143R, E147R, L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pTS107 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-GFP

Plasmid

#199360

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TEV-SUMOEu-EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_SpikeyGi2_kv2.1

Plasmid

#195313

Purpose

Genetically-encoded voltage indicator SpikeyGi2, soma and AIS targeted, in AAV vector under the neuronal-specific promoter hSyn

Depositor

Vincent Pieribone

Article

Platisa et al Nat Methods. 2023 Mar 27. doi: 10.1038/s

Insert

SpikeyGi2

Use

AAV

Promoter

hSyn

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGFP-LacI

Plasmid

#179510

Purpose

Expresses EGFP-LacI in mammalian cells

Depositor

Yasushi Hiraoka

Article

Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

Insert

LacI

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET a sfGCaMP6s

Plasmid

#100022

Purpose

Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

sfGCaMP6s

Tags

6x HIS tag and Xpress_EK tag

Expression

Bacterial

Mutation

cpEGFP replaced with superfolder cpGFP

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pFasBac+GFP-CAMSAP3 CC+CKK

Plasmid

#59041

Purpose

for baculovirus expression of mouse CAMSAP3 CC+CKK in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP3 CC+CKK domain (Camsap3 Mouse)

Tags

His and eGFP

Expression

Insect

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pCLC3sHA

Plasmid

#52424

Purpose

mammalian expression of HA tagged rat CLC3 short transcript

Depositor

Steven Weinman

Article

Wang et al Gastroenterology. 2004 Apr;126(4):1157-6

Insert

CLC3 (Clcn3 Rat)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pX330 _CRISPIE donor B10

Plasmid

#172851

Purpose

DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B10 (Zhong et al, eLife 2021), mEGFP translational phase (2-stop), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

DRS-2 sgRNA and CRISPIE designer exon (phase 2-stop) encoding mEGFP

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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3xnls-cpmTq2-Calcium-lifetime-sensor

Plasmid

#129626

Purpose

Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

3xnls-cpmTq2-Calcium-lifetime-sensor

Tags

3xNLS

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-GLT1b

Plasmid

#162516

Purpose

Rat GLT-1b cDNA N-terminally tagged with EGFP

Depositor

Josef Kittler

Article

Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

Insert

GLT1b (Slc1a2 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pEF Doc2alpha WT GFP

Plasmid

#128819

Purpose

To visualize Doc2alpha WT in PC12 cells

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2alpha (Doc2a Rat)

Tags

EGFP

Expression

Mammalian

Promoter

EF-1alpha

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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