We narrowed to 18,496 results for: Met

1. pROF394

   Plasmid

   #155333

   Purpose

   Expression of PULSE-controlled LFY-VP16-2A-RLuc

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   (etr)8-(C120)5-PhCMVmin-LFY-VP16-NLS-2A-RLuc-TSV40
   

   Use

   Synthetic Biology; Eukaryotic expression

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRW006-SM-destination-pro35S-Cas9

   Plasmid

   #104439

   Purpose

   Destination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

   Depositor

   Detlef Weigel

   Article

   Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial and Plant

   Promoter

   35S

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-17b_Unc104-KIF5B-HALO

   Plasmid

   #169996

   Purpose

   Ultra processive kinesin KIF1A fused with KIF5B stalk for dimerization and HALO-tag for labeling

   Depositor

   Ron Vale

   Article

   Niekamp et al Nat Methods. 2020 Apr;17(4):437-441. doi

   Insert

   Kinesin-like protein Unc-104 fused with KIF5B
   

   Tags

   HALO-tag, 6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHIE053 ZF43x6-C mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

   Plasmid

   #138734

   Purpose

   Integration Vector, assembled with pHIE045 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF43x6-Compact
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. MAC-SARS-CoV-2 M

   Plasmid

   #158369

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   SARS-CoV-2 M (M )
   

   Tags

   MAC-tag

   Expression

   Mammalian

   Available Since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. MAC-SARS-CoV-2 NSP12

   Plasmid

   #158363

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   SARS-CoV-2 NSP12 (ORF1ab )
   

   Tags

   MAC-tag

   Expression

   Mammalian

   Available Since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. MAC-SARS-CoV-2 NSP14

   Plasmid

   #158365

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   SARS-CoV-2 NSP14 (ORF1ab )
   

   Tags

   MAC-tag

   Expression

   Mammalian

   Available Since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. ptdEos-CLC

   Plasmid

   #38009

   Depositor

   Xiaowei Zhuang

   Article

   Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

   Inserts

   Clathrin, light polypeptide (LCa) (Clta Mouse)
   Eos FP, tandem dimer
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. MAC-SARS-CoV-2 NSP11

   Plasmid

   #158362

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   SARS-CoV-2 NSP11 (ORF1ab )
   

   Tags

   MAC-tag

   Expression

   Mammalian

   Available Since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pRK5-HA-SAMTOR (G172A)

    Plasmid

    #100514

    Purpose

    Expresses HA tagged SAMTOR G172A mutant in mammalian cells

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    SAMTOR (BMT2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G172A

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. tdEos-VSVG-16

    Plasmid

    #57693

    Purpose

    Localization: Golgi/ER Transport, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    VSVG
    

    Tags

    tdEos

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pPMVAugw

    Plasmid

    #98300

    Purpose

    Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of CUP1 promoter.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    TEFM1EfmvaE>TEBS1
    

    Expression

    Yeast

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTS2410-Tier1-PhCMV-Myr_mTagBFP2

    Plasmid

    #169535

    Purpose

    Tier-1 vector encoding PhCMV-controlled plasma membrane-tethered mTagBFP2 (PhCMV-myr-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven membrane-localized monomeric Tag blue fluorescent protein 2
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENN.AAV.CB7.CI.PM20D1-flag.WPRE.rBG

    Plasmid

    #132682

    Purpose

    AAV plasmid encoding mouse PM20D1 with C-terminal flag tag

    Depositor

    Jonathan Long

    Article

    Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

    Insert

    Peptidase M20 domain containing 1 (Pm20d1 Mouse)
    

    Use

    AAV

    Tags

    6xHIS and Flag

    Promoter

    Chicken b-actin

    Available Since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENV8(GAAA)-GFPuv

    Plasmid

    #70128

    Purpose

    Negative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loop

    Depositor

    Robert Batey

    Article

    Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:

    Insert

    kissing loop regulatory mutant of environmental cobalamin riboswitch sequence (env8)
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. MDH1-PGK-GFP-miR9

    Plasmid

    #25036

    Depositor

    Bob Weinberg

    Article

    Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

    Insert

    mir-9-3 (MIR9-3 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only

17. MAC-SARS-CoV-2 Orf3a

    Plasmid

    #158372

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 Orf3a (ORF3a )
    

    Tags

    MAC-tag

    Expression

    Mammalian

    Available Since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. Myc-Foxo1-WT 6KQ

    Plasmid

    #17559

    Depositor

    Domenico Accili

    Article

    Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

    Insert

    Foxo1 6KQ (Foxo1 Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

19. Psicheck2 Sox4 full length 3'UTR

    Plasmid

    #26989

    Depositor

    Joan Massague

    Article

    Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

    Insert

    Sox4 Full length 3' UTR (SOX4 Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mammalian

    Available Since

    Jan. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pCytERM-hfYFP

    Plasmid

    #186520

    Purpose

    Visualization of the ER in cells using hyperfolder YFP (compatible with OSER assay)

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Hyperfolder YFP
    

    Tags

    Cytochrome p450 first 29 animo acids (Oryctolagus…

    Expression

    Mammalian

    Mutation

    mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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