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We narrowed to 15,677 results for: Nes

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Showing: 10441 - 10460 of 15677 results

  4. pDual-eGFP(W66)

    Plasmid
    #63217
    Purpose
    Expression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(W66)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed tyrosine at position 66 with respect to t…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pCMV-Myr-HA-mIFITM3-C71,72,105A-CLVL

    Plasmid
    #58414
    Purpose
    Expresses murine IFITM3-C71,72,105A with an N-terminal myristoylation sequence from HIV Gag followed by an HA tag, as well as a C-terminal CaaX prenylation motif, in mammalian cells
    Depositor
    Howard Hang, Jacob Yount
    Article
    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41
    Insert
    IFITM3 (Ifitm3 Mouse)
    Tags
    HA internal
    Expression
    Mammalian
    Mutation
    Cysteines 71, 72, and 105 to Alanine; The sequenc…
    Promoter
    CMV IE
    Available Since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pKG03576_pLentiX1-(TRE-mRuby2-bGH)-cHS4.full-EFS-rtTA-P2A-mGL-WPRE

    Plasmid
    #252560
    Purpose
    Inducible Tet all-in-one circuit with divergent syntax and intergenic cHS4 full insulator, lentiviral vector
    Depositor
    Kate Galloway
    Article
    Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.
    Insert
    rtTA-P2A-mGreenLantern
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EFS (human elongation factor 1-alpha - short/intr…
    Available Since
    April 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    252560-plasmid-map-sequence-id-503972

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  11. pLV-SFFV-CKAP4 CC-WPRE-UbC-Emerald

    Plasmid
    #225950
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutation double cysteine (CC) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Additional cysteine inserted after Cys-100
    Promoter
    SFFV and UbC
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225950-plasmid-map-sequence-id-446852

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  12. pcDNA3-mCherry-TSC2(AA)-NLS

    Plasmid
    #192443
    Purpose
    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.
    Depositor
    Jin Zhang
    Article
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Insert
    mCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …
    Promoter
    CMV
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    192443-plasmid-map-sequence-id-379076

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  17. pAAV-hsyn-DIO-AppNL-G-F-T2A-mCherry

    Plasmid
    #242649
    Purpose
    Floxed (DIO) AAV transgene designed to be packaged in AAV and to express the amyloid precursor protein (App) with familial mutations AppNL-G-F in a Cre-recombinase-dependent manner.
    Depositor
    William Wisden
    Insert
    Floxed (DOI) AAV transgene expressing an amyloid precursor protein with familial mutations and an mCherry tag
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Expression
    Mammalian
    Mutation
    The amyloid precursor reading frame contains a c…
    Promoter
    human synapsin
    Available Since
    Oct. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    242649-plasmid-map-sequence-id-489352

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  19. pcDNA3-mCherry-TSC2(AA,3Q)

    Plasmid
    #192444
    Purpose
    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity.
    Depositor
    Jin Zhang
    Article
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Insert
    mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q ) (TSC2 Human)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …
    Promoter
    CMV
    Available Since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    192444-plasmid-map-sequence-id-379131

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  20. pSLPB2B-SMASh-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187949
    Purpose
    SMASh degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    SMASh-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187949-plasmid-map-sequence-id-373500

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Showing: 10441 - 10460 of 15677 results