164,826 results

1. eMagA-EGFP-Mito

   Plasmid

   #162244

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   eMagA-EGFP-OMP25
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. p5E-mitfa2.1

   Plasmid

   #81234

   Purpose

   zebrafish mitfa promoter entry vector for multisite Gateway cloning

   Depositor

   James Lister

   Insert

   mitfa promoter (mitfa Zebrafish)
   

   Use

   Multisite gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   mitfa, 2.1kb including 5' UTR

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmiRFP680-N1

   Plasmid

   #136557

   Purpose

   A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP680 (another name miRFP713V254C)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   miRFP680
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNPK-NLS-Vika

   Plasmid

   #79969

   Purpose

   expresses human codon-optimized Vika-recombinase in mammalian cells

   Depositor

   Frank Buchholz

   Article

   Karimova et al Nucleic Acids Res. 2013 Jan;41(2):e37. d

   Insert

   human codon-optimized Vika sequence, NLS tagged
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV CMV mCherry-DDX1-V5

   Plasmid

   #175162

   Purpose

   Lentiviral expression of human DDX1-V5

   Depositor

   Larry Gerace

   Article

   Hammond et al J Mol Biol. 2018 Feb 16;430(4):537-553.

   Insert

   DDX1 (DDX1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-mGFAP-Lck-PinkFlamindo

   Plasmid

   #228394

   Purpose

   AAV vector to express the red cAMP indicator in astrocytes

   Depositor

   Tetsuya Kitaguchi

   Article

   Vittani et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

   Insert

   Pink Flamindo
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   mGFAP

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-4T MDM2 WT

   Plasmid

   #16237

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

   Insert

   MDM2 (MDM2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-C-Solo-mStayGold-CANX

   Plasmid

   #227280

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the CANX locus. For ER visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   CANX Homology Arms flanking a mStayGold Tag (CANX Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMJ117

   Plasmid

   #85997

   Purpose

   One-guide Perturb-seq vector backbone; modified human U6 promoter; constant region 3

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   EGFP-NT2_cr3
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   modified human U6

   Available Since

   Feb. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216251

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminus

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgSEC61B.N1 (SEC61B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. SepOTSλ

    Plasmid

    #68292

    Purpose

    Phosphoserine orthogonal translation system (preferred variant)

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    SepRS9/EFSep21, 4xtRNA-Sep-A37
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHO4d-Cas9

    Plasmid

    #67881

    Purpose

    Cas9nlsHis6 Bacterial Expression construct

    Depositor

    Michael Nonet

    Article

    Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and T7 epitope

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVITRO1-Trastuzumab-IgA1/κ

    Plasmid

    #61888

    Purpose

    Expresses HER2/neu receptor specific humanized IgA1/κ antibody isotype (Trastuzumab-IgA1/κ)

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    HER2/neu receptor specific humanized Alpha 1 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mEF1 Prom and rEF1 Prom

    Available Since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3.1(+)-NEMOc

    Plasmid

    #189933

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS

    Plasmid

    #154774

    Purpose

    plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma PylRS for ochre suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    in PylT: UUA anticodon and G10A, G14U, G16U, C20U…

    Promoter

    EF1

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221-SLCO2B1_STOP

    Plasmid

    #161032

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLCO2B1 (SLCO2B1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pAAV_BiSSTe4_GCaMP6f

    Plasmid

    #213947

    Purpose

    AAV construct expressing GCaMP6f driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_GCaMP6f

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Has Service

    AAV PHP.eB and AAV1

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFUSEss-CHIg-mG1_M18

    Plasmid

    #82357

    Purpose

    Expression plasmid coding for heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

    Insert

    mouse immunoglobulin heavy chain IgG1 isotype (Ighg1 Mouse)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    none

    Promoter

    hEF1-HTLV prom

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRRLsinPGK_IRFP720_WPRE

    Plasmid

    #91785

    Purpose

    Constitutive expression of IRFP720 using PGK promoter in a 3rd generation lentiviral backbone.

    Depositor

    Beverly Torok-Storb

    Insert

    iRFP720
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-FLEXfrt-EGFP-KASH

    Plasmid

    #154374

    Purpose

    Vector for Flp-dependent expression of EGFP tagged KASH protein domain

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    EGFP-KASH
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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