We narrowed to 21,629 results for: ESC;

1. HoxD del control ds

   Plasmid

   #131336

   Purpose

   gRNA to delete control region near HoxD. Use with HoxD del control us

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   CTRL-gRNA-C-del
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

2. HoxD del us1

   Plasmid

   #131337

   Purpose

   gRNA to delete a nucleation site near HoxD. Use with HoxD del ds1

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   EVX2-gRNA-N-del
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. HoxD del control us

   Plasmid

   #131335

   Purpose

   gRNA to delete control region near HoxD. Use with HoxD del control ds

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   CTRL-gRNA-N-del
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EZH2-Promoter-gRNA

   Plasmid

   #131329

   Purpose

   gRNA to generate endogenously tagged -tetR-EZH2

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   EZH2-Promoter-gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EED-F97A

   Plasmid

   #131326

   Purpose

   gRNA to generate F97A cage-mutant EED

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   EED-KO-gRNA-2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. EED-KO-gRNA-1

   Plasmid

   #131321

   Purpose

   gRNA to knockout EED. Use with EED-KO-gRNA-2

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   EED-KO-gRNA-1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

7. pCR8_TaNAM-A1

   Plasmid

   #124574

   Purpose

   TaNAM-A1 CDS in the Gateway-compatible pCR8 vector.

   Depositor

   Cristobal Uauy

   Article

   Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

   Insert

   TaNAM-A1
   

   Use

   Topo gateway cloning vector

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMH-HT_ygiF-TTM

   Plasmid

   #127794

   Purpose

   Expresses the TTM/CYTH domain of ygiF in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   ygiF (ygiF Escherichia coli)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   N-terminal TTM domain of ygiF (residues 1-200)

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNIC28-BSA4-S15

   Plasmid

   #127821

   Purpose

   Production of recombinant human ribosomal protein S15

   Depositor

   Ivanhoe Leung

   Article

   Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

   Insert

   RPS15 (RPS15 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNH-TrxT-G-T136D-S15

    Plasmid

    #127824

    Purpose

    Production of recombinant human ribosomal protein S15

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPS15 (RPS15 Human)
    

    Use

    Tags

    His6 - Thioredoxin - TEV

    Expression

    Bacterial

    Mutation

    T136D

    Promoter

    T7

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNH-TrxT-G-S10

    Plasmid

    #127829

    Purpose

    Production of recombinant human ribosomal protein S10

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPS10 (RPS10 Human)
    

    Use

    Tags

    His6 - Thioredoxin - TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJL1-neoR

    Plasmid

    #117048

    Purpose

    In vitro expression of neoR (kanamycin resistance gene) from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    neoR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pClodANL-FGF4

    Plasmid

    #107874

    Purpose

    Bacterial expression of FGF4-NanoLuc

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Insert

    FGF4 (FGF4 Synthetic)
    

    Use

    Luciferase

    Tags

    NanoLuc

    Expression

    Bacterial

    Mutation

    Promoter

    pro D

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAL832

    Plasmid

    #119305

    Purpose

    precusor outer membrane protein A, ompA, truncated after amino acid 176

    Depositor

    Linda Randall

    Article

    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

    Insert

    precursor outer membrane protein A, ompA, truncated after amino acid 176 including with mutations T95C and I153C
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    truncated after amino acid 176, mutations Threoni…

    Promoter

    lac

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAL800

    Plasmid

    #119306

    Purpose

    precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S

    Depositor

    Linda Randall

    Article

    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

    Insert

    precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    amino acids 1 through 171 deleted, and mutatioms …

    Promoter

    lac

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAL928

    Plasmid

    #119853

    Purpose

    precusor alkaline phosphatase, phoA, with C169S, C179S, C287S, C337S, A369C and A427C

    Depositor

    Linda Randall

    Article

    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

    Insert

    precursor alkaline phosphatase, phoA, with mutations C169S, C179S, C287S, C337S, A369C and A427C
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Cysteine 169 to Serine, Cysteine 179 to Serine, C…

    Promoter

    T7

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVITRO-Fab_Theo-LUCID

    Plasmid

    #119048

    Purpose

    Plasmid encoding the mammalian expression of the Theophiline LUCID based on antibodies (BRET semi-synthetic biosensor)

    Depositor

    Kai Johnsson

    Article

    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2

    Inserts

    Fab_Theo-LUCID_part1
    Fab_Theo-LUCID_part2
    

    Use

    Tags

    FLAG-tag, His-tag, and Strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α + CMV/SV40 enhancers and IRES

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVITRO-Fab_Quin-LUCID

    Plasmid

    #119049

    Purpose

    Plasmid encoding the mammalian expression of the Quinine LUCID based on antibodies (BRET semi-synthetic biosensor)

    Depositor

    Kai Johnsson

    Article

    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2

    Inserts

    Fab_Quin-LUCID_part1
    Fab_Quin-LUCID_part2
    

    Use

    Tags

    FLAG-tag, His-tag, and Strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α + CMV/SV40 enhancers and IRES

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVITRO-Fab_MTX-LUCID

    Plasmid

    #119047

    Purpose

    Plasmid encoding the mammalian expression of the Metotraxate LUCID based on antibodies (BRET semi-synthetic biosensor)

    Depositor

    Kai Johnsson

    Article

    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2

    Inserts

    Fab_MTX-LUCID_part1
    Fab_MTX-LUCID_part2
    

    Use

    Tags

    FLAG-tag, His-tag, and Strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α + CMV/SV40 enhancers and IRES

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBAD-MTX-u36_Snifit

    Plasmid

    #119045

    Purpose

    Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 36 (FRET semi-synthetic biosensor)

    Depositor

    Kai Johnsson

    Article

    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-

    Insert

    MTX-u36_Snifit
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    36TAG R44L H45Q

    Promoter

    pBAD

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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