We narrowed to 13,843 results for: CRISPR-Cas9
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Plasmid#165608PurposeVector for expression of the SpCas9 VRKG variant with sgRNA in E. coli: lacUV5-VRKG(SpCas9, D1135V/S1136R/D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)DepositorInsertlacUV5 driving Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G) and hEGFP-sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialMutationD1135V, S1136R, D1332K and R1333G mutations in Sp…PromoterlacUV5 driving Cas9 VRKG and UV5 driving sgRNAAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-RacFRET-U6ac-rac2 guides
Plasmid#168248Purpose"label active Rac under Rac2 neutrophil-specific knockout background"DepositorInsertRacFRET
UseZebrafish expressionPromoterLyzCAvailable SinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
tRNA-gRNA position [n] (GB1207)
Plasmid#75408PurposetRNA and scaffold for the assembly of GBoligomers for the last position (positon [n]) of a polycistronic tRNA-gRNA (2- and 3-part multiplexing)DepositorInserttRNA-gRNA position [n]
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6-1 (GB1204)
Plasmid#75405PurposeProvides the A. thaliana U6-1 RNA polIII promoter as a level 0 GoldenBraid partDepositorInsertAtU6-1 promoter
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPD686 A2UCOE-EnAsCas12a-BFP
Plasmid#249159PurposeOverexpression of EnAsCas12a-BFP with blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site.DepositorInsertA2UCOE-EnAsCas12a-TagBFP
UseCRISPR; Cp36 donor dnaExpressionMammalianPromoterEF1aAvailable SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pX330_NPM2_cterm1
Plasmid#222911PurposeCas9/sgRNA plasmid for targeting NPM2DepositorInsertCas9, NPM2 sgRNA 1 (NPM2 Human, Synthetic)
UseCRISPRAvailable SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330_NPM2_cterm4
Plasmid#222914PurposeCas9/sgRNA plasmid for targeting NPM2DepositorInsertCas9, NPM2 sgRNA 4 (NPM2 Human, Synthetic)
UseCRISPRAvailable SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330_NPM2_cterm3
Plasmid#222913PurposeCas9/sgRNA plasmid for targeting NPM2DepositorInsertCas9, NPM2 sgRNA 3 (NPM2 Human, Synthetic)
UseCRISPRAvailable SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330_NPM2_cterm2
Plasmid#222912PurposeCas9/sgRNA plasmid for targeting NPM2DepositorInsertCas9, NPM2 sgRNA 2 (NPM2 Human, Synthetic)
UseCRISPRAvailable SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-PBD-GFP-U6ac-rac2 guides
Plasmid#168251Purpose"label active Rac under Rac2 neutrophil-specific knockout background"DepositorInsertPBD GFP
UseZebrafish expressionPromoterLyzCAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-Utrophin-GFP-U6ac-Rac2 guides
Plasmid#168254Purpose"label stable F-actin under Rac2 neutrophil-specific knockout background"DepositorInsertUtrophin-GFP
UseZebrafish expressionPromoterLyzCAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-PBD-GFP-U6ac-ctrl guides
Plasmid#168252Purpose"label active Rac; control for neutrophil-specific knockout"DepositorInsertPBD GFP
UseZebrafish expressionPromoterLyzCAvailable SinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-Utrophin-GFP-U6ac-ctrl guides
Plasmid#168253Purpose"label stable F-actin; control for neutrophil-specific knockout"DepositorInsertUtrophin-GFP
UseZebrafish expressionPromoterLyzCAvailable SinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRH2502
Plasmid#84379PurposeExpression of dcas9 D10A H840A from a TetR-regulated uvtetO promoterDepositorInsertdcas9
UseCRISPRExpressionBacterialMutationAspartate at position 10 changed to Alanine, Hist…Promoteruv15tetOAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAtC
Plasmid#78097PurposePlant expression of Cas9 and gRNA empty backbone; Basta resistanceDepositorTypeEmpty backboneUseCRISPRTagsNLS-HAExpressionPlantPromoterU6Available SinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAtC
Plasmid#78098PurposePlant expression of Cas9 and gRNA empty backbone; Hygro ResistanceDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterU6Available SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_RXRB_1
Plasmid#104050PurposeEncodes gRNA for 3' target of human RXRB along with Cas9 with 2A GFPDepositorAvailable SinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_RXRB_2
Plasmid#104051PurposeEncodes gRNA for 3' target of human RXRB along with Cas9 with 2A GFPDepositorAvailable SinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
p305N-31 (nonfunctional)
Plasmid#246314PurposeEvaluation of PtU6.2c4 promoter (nonfunctional) (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter lineDepositorInsertPtU6.2c4 promoter (nonfunctional)
UseCRISPRExpressionPlantMutationdeletions: -A (-41 from TSS), -T (-30), -T (-29);…PromoterPtU6.2c4 promoter (nonfunctional)Available SinceOct. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
p305N-9 (nonfunctional)
Plasmid#246292PurposeEvaluation of AtU6.29c13 promoter (nonfunctional) (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter lineDepositorInsertAtU6.29c13 promoter (nonfunctional)
UseCRISPRExpressionPlantMutationdeletions: -A (-58 from TSS), -T (-57) within USEPromoterAtU6.29c13 promoter (nonfunctional)Available SinceOct. 30, 2025AvailabilityAcademic Institutions and Nonprofits only