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pBAD-HisD-mNG-D-Kbp

Plasmid

#178012

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-D-Kbp

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-D-Kbp

Expression

Bacterial

Promoter

araB

Available Since

Dec. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin.mCherry-P2A-Post-SPHERE-SF-iGluSnFR

Plasmid

#187102

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Post-SPHERE-SF-iGluSnFR

Use

AAV

Tags

mCherry-P2A

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin.TagBFP-P2A-Pre-SPHERE-SF-iGluSnFR

Plasmid

#187101

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Pre-SPHERE-SF-iGluSnFR

Use

AAV

Tags

TagBFP-P2A

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pNOC_hfnCas12a_Nlux_(-HH)crRNA NR1

Plasmid

#176250

Purpose

pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1 without the hammerhead ribozym

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR and Synthetic Biology; Expression in micro…

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pTCas9_scr

Plasmid

#207566

Purpose

ThermoCas9-mediated cleavage of genomic DNA in E. coli

Depositor

John van der Oost

Article

Trasanidou et al Commun Biol. 2023 Oct 13;6(1):1042. doi:

Insert

Plac/tet_ThermoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)

Expression

Bacterial

Mutation

Wild-type

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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Str-2-TagBFP-SL2-Pre-SPHERE-SF-iGluSnFR

Plasmid

#187099

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Pre-SPHERE-SF-iGluSnFR

Tags

TagBFP-SL2

Expression

Worm

Promoter

Str-2

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn RSET-T-GECO1.WPRE

Plasmid

#211902

Purpose

Expresses T-GECO1 in mammalian cells

Depositor

Robert Campbell

Article

Aggarwal et al Neurophotonics. 2024 Apr;11(2):024207. d

Insert

T-GECO1

Use

AAV

Tags

RSET peptide

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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C413-E22: attB4-polyhedrinp>-attB1r

Plasmid

#162922

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression using the AcMNPV polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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PA-ecliptic pHluorin

Plasmid

#137837

Purpose

A photoactivatable GEVI variant originated from Bongwoori-R3

Depositor

Bradley Baker

Article

Lee et al Front Cell Neurosci. 2019 Oct 29;13:482.

Insert

Photoactivatable-ecliptic pHluorin

Expression

Mammalian

Mutation

L64F, T65S, V163A, T203H

Promoter

CMV

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP6f-P2A-Vmn1r86

Plasmid

#133415

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 86

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-vmn1r86 (Vmn1r86 Mouse)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pBS TMEM108

Plasmid

#18852

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Hypothetical protein, Tmem108 (HAPLN1 Chicken)

Expression

Bacterial

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pGEM-T_mouseH3.1-HaloTag

Plasmid

#244242

Purpose

CRISPR/Cas9 donor plasmid to tag mouse histone H3.1 with Halo tag

Depositor

Kazuhiro Maeshima

Article

Minami et al Sci Adv. 2025 Mar 28;11(13):eadu8400. do

Insert

H3.1 (H3c1 Mouse)

Use

CRISPR

Tags

HaloTag

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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p4118

Plasmid

#198151

Purpose

bacterial expression of sseF::HaloTag fusion

Depositor

Michael Hensel

Article

Goser et al Nat Commun. 2023 Mar 4;14(1):1240. doi:

Insert

PsseA::sscB sseF::L16::HaloTag::HA (sseF Salmonella enterica serovar Typhimurium)

Tags

HaloTag-HA

Expression

Bacterial

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-SopB

Plasmid

#183670

Purpose

Inducible Salmonella Typhimurium SopB for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Expression

Yeast

Promoter

GAL1

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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D92_Ld_360170

Plasmid

#135905

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_UG7

Plasmid

#135990

Purpose

Expresses UG7 in mammalian cells.

Depositor

Sumio Terada

Article

Nakai et al Microscopy (Oxf). 2019 Jul 2. pii: 55252

Insert

UtrCH

Tags

helical linker-mEGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-PM-BAF180-SH2

Plasmid

#41932

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BAF180

Use

Retroviral

Expression

Mammalian

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
SBC015866

Plasmid

#226280

Purpose

Expresses BsSfp, MsCAD, and SrCAR from trc promoter. Biosynthesis of (hydroxy)cinnamyl alcohols from (hydroxy)cinnamic acids.

Depositor

Rainer Breitling

Article

Hanko et al Microb Cell Fact. 2023 Nov 18;22(1):238.

Inserts

4'-phosphopantetheinyl transferase Sfp
Cinnamyl alcohol dehydrogenase
Carboxylic acid reductase

Expression

Bacterial

Available Since

Nov. 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLRD13

Plasmid

#208265

Purpose

Production of ent-cassa-12,15-diene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
osKSL7

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD15

Plasmid

#208267

Purpose

Production of ent-sandaracopimaradiene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
osKSL10

Expression

Bacterial

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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