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TtEnd_E448A_pEHISTEV

Plasmid

#179733

Purpose

TtEnd_E448A_pEHISTEV is a mutant form of TtEnd5A_pEHISTEV vector. It expresess mutated TtEnd5A in which Glu448 has been mutated to Ala448.

Depositor

James Naismith

Article

Hussain et al Arch Biochem Biophys. 2025 Feb;764:11027

Insert

TtEnd5A

Tags

6xHis

Expression

Bacterial

Mutation

Glu448 has been mutated to Ala448.

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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TtEnd_E329A_pEHISTEV

Plasmid

#179734

Purpose

TtEnd_E329A_pEHISTEV is a mutant form of TtEnd5A_pEHISTEV vector. It expresess mutated TtEnd5A in which Glu329 has been mutated with Ala329

Depositor

James Naismith

Article

Hussain et al Arch Biochem Biophys. 2025 Feb;764:11027

Insert

TtEnd5A

Tags

6xHis

Expression

Bacterial

Mutation

Glu329 has been mutated to Ala329.

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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TtEnd_H280A_pEHISTEV

Plasmid

#179735

Purpose

TtEnd_H280A_pEHISTEV is a mutant form of TtEnd5A_pEHISTEV vector. It expresess mutated TtEnd5A in which His280 has been mutated with Ala280.

Depositor

James Naismith

Article

Hussain et al Arch Biochem Biophys. 2025 Feb;764:11027

Insert

TtEnd5A

Tags

6xHis

Expression

Bacterial

Mutation

His280 has been mutated to Ala280.

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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TtEnd_CDC_E329A

Plasmid

#179737

Purpose

TtEnd_CDC_E329A expresses TtEnd_CDC_pEHISTEV, in which Glu329 has been mutated with Ala329.

Depositor

James Naismith

Article

Hussain et al Arch Biochem Biophys. 2025 Feb;764:11027

Insert

TtEnd5A

Tags

6xHis

Expression

Bacterial

Mutation

Glu329 has been mutated with Ala329

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pWPI_GW_BioID2_T2A_Blast

Plasmid

#214831

Purpose

Destination vector for cloning BioID2 fusion proteins

Depositor

Hani Goodarzi

Article

Khoroshkin et al Nat Commun. 2024 Sep 9;15(1):7872. doi:

Type

Empty backbone

Use

Lentiviral

Tags

BioID2

Expression

Bacterial and Mammalian

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs-RMRP (pAVA3583)

Plasmid

#239260

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RMRP

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RMRP

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs-RMRP (pAVA3584)

Plasmid

#239261

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RMRP

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RMRP

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET30a-GalK-USP-ET64

Plasmid

#234662

Purpose

Expression an ELP-tagged fusion enzyme of GalK and AtUSP

Depositor

Xianwei Liu

Article

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Inserts

galactokinase
UDP-sugar pyrophosphorylase (USP Mustard Weed)

Tags

ELP

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb827

Plasmid

#217500

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb82 backbone, RK2 ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb837

Plasmid

#217501

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb83 backbone, pBBR1 ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb847

Plasmid

#217502

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb84 backbone, pRO1600/ColE1 ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb867

Plasmid

#217503

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb86 backbone, p15A ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVAb887

Plasmid

#217504

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb88 backbone, pUC ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb897

Plasmid

#217505

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb89 backbone, pBR322/ROP ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-delVP2-VP2-454TAG

Plasmid

#240060

Purpose

Express AAV2 capsid with ncAA mutant at site 454 of VP2

Depositor

Abhishek Chatterjee

Article

Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

Inserts

RC2-DelVP2
VP2-454-TAG

Use

AAV

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-delVP1-VP1-454TAG

Plasmid

#240059

Purpose

Express AAV2 capsid with ncAA mutant at site 454 of VP1

Depositor

Abhishek Chatterjee

Article

Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

Inserts

RC2-DelVP1
VP1-454-TAG

Use

AAV

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-GFP

Plasmid

#238041

Purpose

Encodes sfGFP under lac promoter. Expresses a single guide RNA (under Rha promoter), which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

June 13, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-RFP

Plasmid

#238040

Purpose

Encodes mRFP1 under pLux promoter. Expresses a single guide RNA (under Ara promoter), which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

mRFP1

Use

Synthetic Biology

Promoter

Lux

Available Since

June 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYihI-C-term K-R

Plasmid

#233092

Purpose

Expression of GST-YihI with C-term lysines (K) mutated to arginine (R)

Depositor

Michael Downey

Article

Baijal et al mBio. 2025 Aug 13;16(8):e0050025. doi: 1

Insert

GST-YihI with C-term lysines (K) mutated to arginine (R)

Tags

GST

Expression

Bacterial

Mutation

C-term lysines (K) mutated to arginine (R)

Available Since

May 12, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pRnr-ND

Plasmid

#232579

Purpose

Expression of the Rnr nuclease domain (residues 649 to 1929) as a GST-fusion.

Depositor

Michael Downey

Article

Baijal et al mBio. 2025 Aug 13;16(8):e0050025. doi: 1

Insert

Rnr nuclease domain (residues 649 to 1929)

Tags

GST

Expression

Bacterial

Available Since

May 12, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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