169,392 results

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pdCas9-GFP-VPR

Plasmid

#181907

Purpose

Fluorescently tagged dead Cas9 fused to the tripartite transcriptional activator VP64-p65-Rta

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

dCas9-VPR

Use

CRISPR

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pH-ePPE

Plasmid

#183097

Purpose

For plant prime editing in rice plants

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

Insert

Nls-nCas9-NC-nls-MLV-Nls

Use

CRISPR

Expression

Plant

Mutation

D10A in SpCas9

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pKD236-4b

Plasmid

#90956

Purpose

Expresses ThsS constitutively

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Insert

ThsS

Expression

Bacterial

Promoter

Bba_J23104

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pBetaActin-mNeonGreen-CAMSAP3

Plasmid

#191322

Purpose

Expresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flow

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

mNeonGreen-CAMSAP3 (Camsap3 Mouse)

Expression

Mammalian

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-LAMP1-iLID-Halo

Plasmid

#247296

Purpose

Mammalian expression of LAMP1-iLID-Halo fusion for light-induced recruitment to late endosomes/lysosomes.

Depositor

Jennifer Lippincott-Schwartz

Article

Choi et al Nature. 2025 Nov 5. doi: 10.1038/s41586-

Insert

LAMP1-iLID-Halo

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mCherry-sspB-Sec61

Plasmid

#247297

Purpose

Mammalian expression of mCherry-sspB-Sec61 fusion; for optogenetic control of protein recruitment to the ER

Depositor

Jennifer Lippincott-Schwartz

Article

Choi et al Nature. 2025 Nov 5. doi: 10.1038/s41586-

Insert

mCherry-sspB-Sec61

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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PL-SIN-EF1α-EGFP

Plasmid

#21320

Purpose

Control Lentiviral vector with strong EF1a promoter, expresses EGFP

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Expression

Mammalian

Promoter

EF1α

Available Since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pLentiCas9-T2A-BFP

Plasmid

#78547

Purpose

Cas9 protein with BFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

In frame with Cas9

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-BBS1

Plasmid

#218711

Purpose

Expresses N-terminally EGFP-tagged BBS1 in mammalian cells

Depositor

Kazuhisa Nakayama

Insert

BBS1 (BBS1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCM433kanT

Plasmid

#61262

Purpose

Sucrose counterselection (sacB) vector for unmarked allelic exchange.

Depositor

Mary Lidstrom

Article

Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

Type

Empty backbone

Use

Synthetic Biology; Sucrose counterselection

Expression

Bacterial

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only

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