159,557 results

1. pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.GPI

   Plasmid

   #196223

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   pAAV hSyn FLP-FRT iGluSnFR3 v857.GPI
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   GPI anchor, IgK-chain, and Myc epi tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-hARdelta538-614

   Plasmid

   #89107

   Purpose

   mammalian expression of human androgen receptor with deletion of amino acids 538-614, deletion of AR DNA binding domain

   Depositor

   Elizabeth Wilson

   Article

   Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23

   Insert

   hAR-538-614 (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hAR delta 538-614

   Promoter

   CMV

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

3. tsPurple chromoprotein

   Plasmid

   #117848

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses tsPurple chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, tsPurple
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

   Plasmid

   #139090

   Purpose

   Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   dSpCas9-VPR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. EBFP2-H2B-6

   Plasmid

   #55243

   Purpose

   Localization: Nucleus/Histones, Excitation: 383, Emission: 448

   Depositor

   Michael Davidson

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   EBFP2

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAAV-CBh-mKate2-IRES-MCS (WT-WT)

   Plasmid

   #105921

   Purpose

   AAV-mediated gene expression from CBh promoter. Contains canonical left and right AAV2 ITRs

   Depositor

   Marcella Patrick

   Has Service

   AAV2

   Inserts

   mKate2
   IRES-Multiple cloning site
   

   Use

   AAV

   Tags

   Expression

   Mutation

   codon optimized

   Promoter

   CBh and same CBh promoter as mKate2

   Available since

   Feb. 15, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. COVID-SARS2 NSP13

   Plasmid

   #159614

   Purpose

   Bacterial expression plasmid for COVID-SARS2 NSP13 helicase

   Depositor

   Opher Gileadi

   Article

   Newman et al Nat Commun. 2021 Aug 11;12(1):4848. doi:

   Insert

   Covid-SARS2 Nsp13
   

   Use

   Tags

   His6-Zb-TEV

   Expression

   Bacterial

   Mutation

   Codon-optimized for E. coli expression

   Promoter

   T7 - LacO

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-hARdeltaNH2-6

   Plasmid

   #89104

   Purpose

   mammalian expression of human androgen receptor amino acids 507-919

   Depositor

   Elizabeth Wilson

   Article

   Simental et al J Biol Chem. 1991 Jan 5;266(1):510-8.

   Insert

   hAR DNA binding domain+ligand binding domain (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NH2 terminal deletion, contains amino acids 507-…

   Promoter

   CMV

   Available since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXs-3XFLAG-EGFP-OMP25

   Plasmid

   #83354

   Purpose

   For tagging mitochondria with Sigma FLAG epitopes

   Depositor

   David Sabatini

   Article

   Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

   Insert

   3XFLAG-EGFP-OMP25
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. FSW_jGCaMP8f

    Plasmid

    #197034

    Purpose

    Express GCaMP8f from hSyn1 promoter

    Depositor

    Ronald Hart

    Insert

    GCaMP8f
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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