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Talin1-mCherry

Plasmid

#212004

Purpose

Expression of mouse full-length (1-2541) talin1-mCherry in mammalian cells

Depositor

Vesa Hytönen

Article

Rahikainen et al J Cell Sci. 2019 Apr 3;132(7):jcs226514.

Insert

Mouse talin1 (Tln1 Mouse)

Tags

mCherry fluorescent protein

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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EK0285 SFFV-mCherry-SG(s70587)SG-EGFP (FLP-IN)

Plasmid

#191140

Purpose

Expression of Sensor 1 (for EK0208) in mammalian cells; mCherry marker, EGFP output.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:s70587:EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLOX-TERT-iresTK

Plasmid

#12245

Purpose

2nd generation lentiviral vector for reversible immortalization of human primary cells

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

TERT, HSV1-TK (TERT Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

LoxP in 3' LTR.

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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CIRTS-1: ORF5-TBP6.7-Pin domain-NLS

Plasmid

#132543

Purpose

Expresses CIRTS-1 (ORF5-TBP6.7-Pin domain-NLS) in mammalian cells

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

CIRTS-1: ORF5-TBP6.7-Pin domain-NLS

Tags

FLAG tag

Expression

Mammalian

Promoter

cmv d0

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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Nb-gp41-GFP (MoonTag-Nb-GFP)

Plasmid

#128602

Purpose

Expresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

Nanobody_gp41

Use

Lentiviral

Tags

GB1 and sfGFP

Promoter

SFFV

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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Red Glifon Nega

Plasmid

#163117

Purpose

Red fluorescent protein-based glucose negative control sensor (does not respond to glucose) for mammalian cells

Depositor

Takashi Tsuboi

Article

Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

Insert

Red Glifon Nega

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKP113 (pRS406-PRC1p-PRC1-GFP-ADH1t)

Plasmid

#106464

Purpose

Expresses Prc1 with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

PRC1

Tags

GFP

Expression

Bacterial and Yeast

Promoter

PRC1

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-mito

Plasmid

#174180

Purpose

Express AcGFP1 with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

AcGFP with mitochondria-targeting sequence

Tags

the mitochondria-targeting sequence of the cytoch…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-ClpX

Plasmid

#234733

Purpose

Human-codon optimized E. coli ClpX in mammalian cells

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

ClpX

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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Nb-gp41-Halo (MoonTag-Nb-Halo)

Plasmid

#128603

Purpose

Expresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

Nanobody_gp41

Use

Lentiviral

Tags

GB1 and Halo

Promoter

SFFV

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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Pyronic/pcDNA3.1(-)

Plasmid

#51308

Purpose

Pyronic, FRET sensor that allow quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resolution.

Depositor

Luis Felipe Barros

Article

San Martin et al PLoS One. 2014 Jan 21;9(1):e85780. doi:

Insert

Pyronic

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2014

Availability

Academic Institutions and Nonprofits only

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LZRS-IresGFP

Plasmid

#21961

Depositor

Lynda Chin

Article

Kim et al Cell. 2006 Jun 30. 125(7):1269-81.

Insert

Ires GFP

Use

Retroviral

Expression

Mammalian

Mutation

GACAGATGACTATGCAGAGAT

Available Since

Sept. 3, 2009

Availability

Academic Institutions and Nonprofits only
pCMV 3xFLAG-LC3B WT

Plasmid

#123092

Purpose

Expresses 3xFLAG-LC3B wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO APEX2-eIF4E1

Plasmid

#129644

Purpose

Express APEX2 fusion to cap-binding protein eIF4E1 in mammalian cells

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

EIF4E1 (EIF4E Human)

Tags

APEX2

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3/hArf1(WT)-EGFP

Plasmid

#79413

Purpose

Expresses C-terminally EGFP-tagged Arf1(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf1 (ARF1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-mScarlet3-CAAX-IRES-Puro

Plasmid

#222639

Purpose

Live-cell plasma membrane marker

Depositor

Arnold Hayer

Article

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

mScarlet3-CAAX

Use

Lentiviral

Tags

mScarlet3

Promoter

EF1a

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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PB533-42B3mutAC2-sfGFP

Plasmid

#186777

Purpose

RNAP2 Ser2ph-mintbody [42B3(R78K/A80T/M95V)-scFv] expression vector for mammalian cells

Depositor

Hiroshi Kimura

Article

Uchino et al J Cell Biol. 2022 Feb 7;221(2). pii: 212

Insert

42B3mutAC2-scFv

Tags

sfGFP

Expression

Mammalian

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-mSTING

Plasmid

#229739

Purpose

Expresses mouse STING in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

STING (Sting1 Mouse)

Tags

None

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAPL2 WT

Plasmid

#123103

Purpose

Expresses 3xFLAG-GABARAPL2 wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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CAGGS-MCS-NLS-3XFLAG-V5

Plasmid

#196383

Purpose

Empty backbone vector to insert cDNA for strong expression in mammalian cells

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Type

Empty backbone

Tags

3xFLAG and V5

Expression

Bacterial and Mammalian

Promoter

CAGGs

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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