We narrowed to 718 results for: HL;

1. mCardinal-MCAK-C-7

   Plasmid

   #56168

   Purpose

   Localization: Microtubules/Kinesin, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   MCAK (KIF2C Human)
   

   Use

   Tags

   mCardinal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

2. p-miR-Report + WT miR-181 BS in GATA6 3UTR

   Plasmid

   #22794

   Depositor

   Xin Wang

   Article

   Ji et al Hepatology. 2009 Aug . 50(2):472-80.

   Insert

   Wild-type miR-181 Binding Site in GATA binding protein 6 3 Untranslated Region (GATA6 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 24, 2010

   Availability

   Academic Institutions and Nonprofits only

3. p-miR-Report + MT miR-181 BS in GATA6 3UTR

   Plasmid

   #22795

   Depositor

   Xin Wang

   Article

   Ji et al Hepatology. 2009 Aug . 50(2):472-80.

   Insert

   Mutant miR-181 Binding Site in GATA binding protein 6 3 Untranslated Region (GATA6 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 24, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pHL-EF1a-SphcCas9(D10A)-iP-A

   Plasmid

   #60600

   Purpose

   Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

   Depositor

   Akitsu Hotta

   Article

   Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

   Inserts

   CRISPR Cas9 D10A
   puromycin resistance gene
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Changed Asp 10 to Ala, Codon usage optimized for …

   Promoter

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.3-hALDOA D34S

   Plasmid

   #210810

   Purpose

   To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

   Depositor

   Sheng-cai Lin

   Article

   Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

   Insert

   ALDOA (ALDOA Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   residues 34 of ALDOA mutated to serine

   Promoter

   CMV

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCardinal-Calnexin-N-14

   Plasmid

   #56154

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   Calnexin (CANX Human)
   

   Use

   Tags

   mCardinal

   Expression

   Mammalian

   Mutation

   I474N in Calnexin

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. mCardinal-Fibrillarin-7

   Plasmid

   #56160

   Purpose

   Localization: Nuclear - Nucleolus, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   Fibrillarin (FBL Human)
   

   Use

   Tags

   mCardinal

   Expression

   Mammalian

   Mutation

   R36Q, S66F

   Promoter

   CMV

   Available Since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pMT-HGP-v3-Nb127D01-hIgG

   Plasmid

   #171564

   Purpose

   Expresses hIgG-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-human IgG-TEV-Avi-His) in fly cells

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   Nb127D01-human IgG-TEV-Avi-His (CXCR2 Human)
   

   Use

   Tags

   BiP signal peptide and hIgG1-hinge-N297A-TEV site…

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCJ199

   Plasmid

   #162672

   Purpose

   pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with C-terminal His tag, codon optimized for expression in E. coli K12.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   Putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with signal peptide
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   codon optimized for expression in E. coli K12

   Promoter

   T7/lac

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCJ203

    Plasmid

    #162678

    Purpose

    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    Putative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deltaF2-A75; codon optimized for expression in E.…

    Promoter

    T7/lac

    Available Since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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