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We narrowed to 2,481 results for: D10

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Showing: 1041 - 1060 of 2481 results

  1. lenti-dCas9-VP64-BFP

    Plasmid
    #196711
    Purpose
    CRISPR-activation. dCas9-VP64 with TagBFP for sorting
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-VP64-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    196711-plasmid-map-sequence-id-392561

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  2. mCh-KIF5A*-strep

    Plasmid
    #120163
    Purpose
    Expresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidin
    Depositor
    Juan Bonifacino
    Publication
    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    kinesin family member 5A (Kif5a Mouse)
    Tags
    Streptavidin and mCherry
    Expression
    Mammalian
    Mutation
    Truncated KIF5A: 1-559 aa
    Promoter
    CMV
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    120163-plasmid-map-sequence-id-247481

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  4. pSLPB2B-FKBP12_F36V-SpdCas9-KRAB-tagBFP-PGK-Blasticidin

    Plasmid
    #187953
    Purpose
    FKBP12 (F36V mutant) degron-tagged dCas9-KRAB domain fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP12_F36V-SpdCas9-KRAB-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187953-plasmid-map-sequence-id-373487

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  7. pWT065a

    Plasmid
    #107912
    Purpose
    W2m.3.1-1 plasmid. Contains (TCF-LEF)7-BE3 and is ppart of the CAMERA2m system for cellular recording in mammalian cells
    Depositor
    David Liu
    Publication
    Tang et al Science. 2018 Feb 15. pii: science.aap89
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    (TCF-LEF)7-BE3
    Expression
    Mammalian
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107912-plasmid-map-sequence-id-264842

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  9. LLP791_PB_dCas9-SSSavi_BirA

    Plasmid
    #211786
    Purpose
    PiggyBac plasmid with dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), and BirA, with Hygro selection
    Depositor
    Ryan Lister
    Publication
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9, Spy, Snoop, aGCN4, AviTags
    Tags
    3xHA
    Expression
    Mammalian
    Mutation
    dCas9 D10A and H840A
    Promoter
    pCAGG abd pEF1a
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    211786-plasmid-map-sequence-id-417395

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  11. pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-dSaCas9-2xNLS

    Plasmid
    #107319
    Purpose
    Expresses SpCas9 fused to dSaCas9 in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-SaCas9 fusion
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SpCas9 is fused to nuclease dead SaCas9 (D10A, N5…
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107319-plasmid-map-sequence-id-204682

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  12. pcDNA5FRT/TO-SAKS1-Strep-HA

    Plasmid
    #113487
    Purpose
    Expression of human SAKS1 with C-terminal strep-HA tag
    Depositor
    Hemmo Meyer
    Publication
    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SAKS1 (UBXN1 Human)
    Tags
    2xstrep-HA
    Expression
    Mammalian
    Mutation
    wildtype without stop codon
    Promoter
    CMV
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113487-plasmid-map-sequence-id-218561

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  14. pAC1-pCR8-dCas9VP48

    Plasmid
    #48214
    Purpose
    dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP48 activation domain
    Use
    CRISPR and Gateway Cloning
    Tags
    HA Tag and VP48
    Mutation
    D10A;H840A
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48214-plasmid-map-sequence-id-69493

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  18. pMH220

    Plasmid
    #122836
    Purpose
    dCas9 with GreenGate B-C flanking sequences
    Depositor
    Markus Schmid
    Publication
    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
    Use
    Golden gate compatible cloning vector
    Mutation
    Contains D10A and H840A mutations
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122836-plasmid-map-sequence-id-239365

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Showing: 1041 - 1060 of 2481 results