We narrowed to 17,082 results for: EMB

1. pHAND1-AM-Tag-PGK-Neo

   Plasmid

   #223216

   Purpose

   Targeting plasmid for inserting a C-terminal AM-Tag in the human HAND1 gene

   Depositor

   Matthew Birket

   Article

   Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

   Insert

   HAND1 (HAND1 Human)
   

   Use

   Cre/Lox; Human targeting

   Tags

   AM-Tag

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBiFC-SS-VC155-IRE1-dLD

   Plasmid

   #217751

   Purpose

   Expresses the VC fragment of BiFC-IRE1-dLD

   Depositor

   Christina Chan

   Article

   Cho et al Cell Rep. 2019 Apr 2;27(1):307-320.e5. d

   Insert

   VC155-IRE1-dLD (ERN1 Human)
   

   Tags

   Venus (155-238, A206K)

   Expression

   Mammalian

   Mutation

   deleted amino acids 29-408

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

   Plasmid

   #224512

   Purpose

   A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   3xHA-BirA-T2A-mCherry-Caax
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-CD28(CD45TM)

   Plasmid

   #223626

   Purpose

   Lentiviral expression of human CD28 (CD45TM) in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   CD28 (CD28 Human)
   

   Use

   Lentiviral; mammalian expression

   Mutation

   CD28 transmembrane domain replaced by CD45 transm…

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pS238D1::sptse5-CT_phoA-lacZalpha

   Plasmid

   #192961

   Purpose

   Test the biological function of the spTse5-CT (1169-1317)-PhoA (22-474)-LacZ (4-60) fusion protein in P. putida

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   sptse5-CT_phoA-lacZ_
   

   Tags

   PelB signal peptide

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1317) fused to phoA-lacZal…

   Available Since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330_gRNA_H19-DMR-2

   Plasmid

   #159930

   Purpose

   Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse H19-DMR (H19-icr Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6, CBh

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330_gRNA_H19-DMR-1

   Plasmid

   #159929

   Purpose

   Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse H19-DMR (H19-icr Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6, CBh

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKI-cjACTB-EGFP-3'short

   Plasmid

   #129749

   Purpose

   Gene targeting in marmoset cells

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

   Insert

   ACTB (ACTB C. jacchus (common marmost))
   

   Use

   Marmoset targeting

   Tags

   EGFP

   Available Since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKI-cjACTB-EGFP-5'short

   Plasmid

   #129748

   Purpose

   Gene targeting in marmoset cells

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

   Insert

   ACTB (ACTB C. jacchus (common marmost))
   

   Use

   Marmoset targeting

   Tags

   EGFP

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKI-cjACTB-EGFP

    Plasmid

    #129747

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    ACTB (ACTB C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Tags

    EGFP

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3_nmPKA-CαN-mPAGFP

    Plasmid

    #114130

    Purpose

    Mammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP, with G2A mutation (Prkaca Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-N1 Dynamin1 T78R L84R T92R V118R

    Plasmid

    #112106

    Purpose

    Mammalian expression plasmid of GFP-tagged Dynamin protein.

    Depositor

    Justin Taraska

    Article

    Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

    Insert

    Dynamin1 (DNM1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T78R L84R T92R V118R

    Promoter

    CMV

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-CMV-EGFP-PolB-Hygro

    Plasmid

    #176056

    Purpose

    EGFP fused to the N-terminus of PolB & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag_HsIRE1a_pBabePuro

    Plasmid

    #54337

    Purpose

    N-terminally FLAG tagged human IRE1a in retroviral pBabe puro vector

    Depositor

    David Ron

    Article

    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

    Insert

    IRE1a (ERN1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and preprotrypsin signal sequence

    Expression

    Mammalian

    Mutation

    The endogenous IRE1a signal sequence (aa1-18) was…

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETDuet-1 TIM9,10

    Plasmid

    #170280

    Purpose

    Expresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

    Depositor

    Paul Schanda

    Article

    Weinhaupl et al Cell. 2018 Nov 15;175(5):1365-1379.e25.

    Insert

    mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag) (TIM9, TIM10 Budding Yeast)
    

    Expression

    Bacterial

    Promoter

    Tim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFOXA2-T2A-mTagBFP2-PGK-Neo

    Plasmid

    #223191

    Purpose

    BFP reporter plasmid targeting the human FOXA2 gene

    Depositor

    Matthew Birket

    Article

    Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

    Insert

    FOXA2-T2A-mTagBFP2-PGK-Neo-FOXA2 (FOXA2 Human)
    

    Use

    Cre/Lox; Human targeting

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCW57.1-Eomes-3xT7

    Plasmid

    #200888

    Purpose

    Inducible lentiviral expression of Eomes

    Depositor

    Kai Ge

    Article

    Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

    Insert

    Eomes (Eomes Mouse)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    3xT7

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDyn1

    Plasmid

    #64067

    Purpose

    Human cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for Sf9 expression with a SNAPf tag for fluorescent labeling and a TEV-cleavable His-ZZ tag for purification (His-ZZ-TCS-SNAPf-DYNC1H1).

    Depositor

    Andrew Carter

    Article

    Schlager et al EMBO J. 2014 Sep 1;33(17):1855-68. doi:

    Insert

    DYNC1H1 (DYNC1H1 Human)
    

    Use

    Cre/Lox

    Tags

    His-ZZ-TCS-SNAPf

    Expression

    Insect

    Mutation

    Codon optimized for Sf9

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCW57.1 _3xHA-Ago2

    Plasmid

    #73538

    Purpose

    Inducible lentiviral expression of Ago2

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    Ago2 (Ago2 Mouse)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2+-N-PAS2-GAF-PHYB-MCherry-CAAX

    Plasmid

    #154910

    Purpose

    1-621 amino acids of Arabidopsis PHYB (Gene ID: 816394), tagged with mCherry fluorescence protein and the CAAX membrane moiety. Mammalian codon optimised.

    Depositor

    Jonathan Clarke, Clare Buckley

    Article

    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

    Insert

    Phytochrome B (PHYB Mustard Weed, Synthetic)
    

    Use

    Xenopus/avian/zebrafish

    Tags

    CAAX membrane moiety and mCherry

    Expression

    Mammalian

    Mutation

    1-621 amino acids of Arabidopsis PHYB

    Promoter

    CMV

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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