We narrowed to 42,011 results for: LAS
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Plasmid#105541PurposeAAV expression of EGFP from CamKII promoterDepositorHas ServiceAAV1 and AAV5InsertEGFP
UseAAVExpressionMammalianPromoterCamkIIAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)
Plasmid#185912PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpRY-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…PromoterCMV and T7Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP
Plasmid#188899PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening elementDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusionPromoterSFFVAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Blast
Plasmid#111887PurposeLentiviral expression of cDNA with blasticidin resistance geneDepositorTypeEmpty backboneUseLentiviralTagsFLAGExpressionMammalianPromoterEFS promoterAvailable SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.hSyn.TurboRFP.WPRE.RBG
Plasmid#105552PurposeAAV expression of TurboRFP from hSyn promoterDepositorHas ServiceAAV1InsertTurboRFP
UseAAVExpressionMammalianPromoterhSynAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(E123A)-mCherry
Plasmid#35506PurposeAAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.DepositorInserthChR2
UseAAVTagsmCherryExpressionMammalianMutationE123APromoterCaMKIIaAvailable SinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
PCR4-Shh::lacZ-H11
Plasmid#139098PurposeenSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingExpressionMammalianPromoterShhAvailable SinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO hChR2(E123A)-EYFP
Plasmid#35507PurposeAAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.DepositorInserthChR2
UseAAVTagsEYFPExpressionMammalianMutationE123APromoterEf1aAvailable SinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
UBC plasmid
Plasmid#169746PurposeUnregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.DepositorInsertEGFP
UseSynthetic BiologyExpressionMammalianPromoterUBCAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE30-butelase1
Plasmid#115430PurposeExpresses the macrocyclizing asparaginyl endopeptidase butelase 1 in E. coliDepositorInsertbutelase 1
Tags6-HisPromoterT5Available SinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cytoplasmic cpGFP
Plasmid#215709PurposeExpresses cpGFP in the cytoplasmDepositorInsertcpGFP
UseLentiviralTagsFLAG, HA, and cytoplasmic localization sequenceExpressionMammalianMutationcircularly permuted GFPPromoterCMVAvailable SinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tn7 helper plasmid
Plasmid#141161PurposeTemperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoterDepositorInsertTn7 transposon machinery
ExpressionBacterialPromoterArabinose-inducible pBADAvailable SinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-LbCpf1-Blast
Plasmid#84751PurposeExpresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.DepositorInsertLbCpf1
UseCRISPR and LentiviralTagsNLS-3xHA (C terminal on insert)ExpressionMammalianPromoterEFS-NSAvailable SinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-ccdB-Blast
Plasmid#158560PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
PGK plasmid
Plasmid#169744PurposeUnregulated "open-loop" PGK promoter plasmid. This plasmid encodes eGFP to report circuit output levels.DepositorInserteGFP
UseSynthetic BiologyExpressionMammalianPromoterPGK promoterAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
cytoplasmic cpVenus control
Plasmid#186788PurposeIdentical to cytoplasmic NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligaseDepositorInsertcytoplasmic cpVenus control
UseLentiviralExpressionMammalianAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash5
Plasmid#74133PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 of Arrestin.DepositorInsertArrestin3
TagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…Available SinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash6
Plasmid#74134PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 410 of Arrestin3.DepositorInsertArrestin3
TagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…Available SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
LVXN-Neo-NSD2
Plasmid#86010Purposeexpress wild-type NSD2 in mammalian cellsDepositorInsertNSD2 (NSD2 Human)
UseLentiviralTagsFlag tag D Y K D D D D KExpressionBacterialMutationK1002R (please see depositor comments below)PromoterCMVAvailable SinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLeAPS-GFP-blast
Plasmid#182230PurposeLentiviral transfer plasmid for the LeAPS packaging system; encodes GFP-P2A-blasticidin transgeneDepositorInsertGFP-P2A-Blasticidin
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only