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pmCellFree_KA2_ZIKV C

Plasmid

#145359

Purpose

Cell-free/mammalian expression vector for Zika virus capsid protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV Capsid

Use

Tags

8xHis and mCherry

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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MAC_N_ZIC3

Plasmid

#167809

Purpose

Expresses ZIC3 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

ZIC3 (ZIC3 Human)

Use

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_EPHB6

Plasmid

#187763

Purpose

MAC-tagged gene expression of human EPHB6

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

EPHB6 (EPHB6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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BirA-Myc_N_HEN1

Plasmid

#167708

Purpose

Expresses HEN1 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

HEN1 (NHLH1 Human)

Use

Tags

Myc-BirA

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pGENE:EFF-1::mCherry::V5

Plasmid

#133020

Purpose

EFF-1 fused to mCherry fluorescent protein cloned into the pGene vector for inducible expression in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Perez-Vargas et al Cell. 2014 Apr 10;157(2):407-419. doi: 1

Insert

EFF-1::mCherry::V5

Use

Tags

V5; mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV PrM

Plasmid

#145360

Purpose

Cell-free/mammalian expression vector for Zika virus PrM protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV PrM

Use

Tags

8xHis and mCherry

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

March 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G06_ZIKV NS3

Plasmid

#145355

Purpose

Cell-free expression vector for Zika virus NS3 protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS3

Use

Tags

mCherry and 8xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_N_ETV7

Plasmid

#167756

Purpose

Expresses ETV7 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

ETV7 (ETV7 Human)

Use

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 Orf9c

Plasmid

#158379

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf9c

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC_C_INSR

Plasmid

#187788

Purpose

MAC-tagged gene expression of human INSR

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

INSR (INSR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV NS4B

Plasmid

#145367

Purpose

Cell-free/mammalian expression vector for Zika virus NS4Bprotein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4B

Use

Tags

8xHis and mCherry

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-3GFP-kanMX6

Plasmid

#105148

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu, Thomas Pollard, David Kovar

Article

Wu et al J Cell Biol. 2006 Jul 31;174(3):391-402.

Insert

3GFP-kanMX6

Use

Tags

3GFP

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1_ZIKV NS2B

Plasmid

#145344

Purpose

Cell-free/ mammalian expression vector for Zika virus NS2B protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2B

Use

Tags

8xHis and eGFP

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCellFree_KA1_ZIKV NS4B

Plasmid

#145347

Purpose

Cell-free/ mammalian expression vector for Zika virus NS4B protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4B

Use

Tags

8xHis and eGFP

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G06_ZIKVC

Plasmid

#145349

Purpose

Cell-free expression vector for Zika virus Capsid protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV Capsid

Use

Tags

mCherry and 8xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC-SARS-CoV-2 Orf3b

Plasmid

#158373

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf3b

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pmCellFree_KA1_ZIKV NS1

Plasmid

#145342

Purpose

Cell-free/mammalian expression vector for Zika virus NS1 protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS1

Use

Tags

8xHis and eGFP

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G06_ZIKV NS1

Plasmid

#145352

Purpose

Cell-free expression vector for Zika virus NS1 protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS1

Use

Tags

mCherry and 8xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCellFree_KA2_ZIKV NS4A

Plasmid

#145366

Purpose

Cell-free/mammalian expression vector for Zika virus NS4A protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4A

Use

Tags

8xHis and mCherry

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCellFree_KA1_ZIKV PrM

Plasmid

#145340

Purpose

Cell-free/mammalian expression vector for Zika virus PrM protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV PrM

Use

Tags

8xHis and eGFP

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

April 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge