We narrowed to 3,702 results for: WOL

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pSMART-ala13

Plasmid

#87174

Purpose

NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Constitutive proD

Available Since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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pSMART-ala11

Plasmid

#87172

Purpose

NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Constitutive proA

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSMART-ala12

Plasmid

#87173

Purpose

NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Constitutive proC

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet-I3-SYFP2

Plasmid

#189763

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-I3-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I3-SYFP2

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet3

Plasmid

#189754

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet3 red fluorescent protein

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet-I3

Plasmid

#189757

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-I3 red fluorescent protein

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I3

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPRi

Plasmid

#199808

Purpose

vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

Depositor

Steve Salipante

Article

Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

Inserts

dCas9
sgRNA with GFP cassette

Use

CRISPR

Promoter

P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet3-SYFP2

Plasmid

#189764

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet3-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-SYFP2

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2 GFP-GSK3-MAPK

Plasmid

#29689

Purpose

GFP reporter protein fused to three GSK3 phosphorylation sites

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

GFP glycogen synthase kinase 3 recognition site primed by MAPK

Tags

Flag and Flag-EGFP

Expression

Mammalian

Mutation

recognition sites only, and E3 ligase site

Promoter

SP6

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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pCS2 GFP-GSK3mut-MAPK

Plasmid

#29690

Purpose

GFP reporter protein fused to three mutated GSK3 phosphorylation sites

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

GFP glycogen synthase kinase 3 recognition site mutated primed by MAPK

Tags

Flag and Flag-EGFP

Expression

Mammalian

Mutation

recognition sites only, and E3 ligase site, GSK3 …

Promoter

SP6

Available Since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pGL4.18 CMV-Luc

Plasmid

#100984

Purpose

CMV luciferase reporter - firefly luciferase driven by CMV promoter

Depositor

Lee Helman

Article

Grohar et al J Natl Cancer Inst. 2011 Jun 22;103(12):

Insert

CMV

Expression

Mammalian

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2FA-H2B-mScarlet3

Plasmid

#224436

Purpose

Construct for labelling cell nuclei in mammalian cells using Histon protein 2B fused to mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

H2B-mScarlet3

Expression

Mammalian

Promoter

CMV & SP6

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS613_pTetR-P2A-BFPnls/sgTelo

Plasmid

#108649

Purpose

Telomere-targeting Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Telomere-targeting sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pRDA_816

Plasmid

#201157

Purpose

EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRa

Depositor

John Doench

Article

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

5x-ALFA tag-dEnAsCas12a-VP64

Use

CRISPR and Lentiviral

Mutation

D908A

Promoter

EFS

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Syn1-mCherry

Plasmid

#222967

Purpose

Make lentivirus to express mCherry in syn1 expressing cells

Depositor

Xinyu Zhao

Article

Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

Insert

mCherry

Use

Lentiviral

Promoter

synapsin I

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet-SYFP2

Plasmid

#189762

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-SYFP2

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2 HRS-RFP

Plasmid

#29685

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

hepatocyte growth factor-regulated tyrosine kinase substrate (HGS Human)

Tags

RFP

Expression

Mammalian

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pEJS614_pTetR-P2A-BFPnls/sgNS

Plasmid

#108650

Purpose

Non-specific Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Non-specific sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 Ub-S297

Plasmid

#169965

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 C-terminus fused to ubiquitin (co-translationally processed)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Tags

Ub

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDx_mCherry-SYFP2

Plasmid

#189759

Purpose

Dual expression vector for bacteria and mammalian cells producing mCherry-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mCherry-SYFP2

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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