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Plasmid#217955PurposeExpression of human H3.1 tagged on its C-terminus with eGFP. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H3.1 (H3C1 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pH2B-miRFP718nano
Plasmid#195750PurposeHistone 2B labelled with near-infrared fluorescent protein miRFP718nanoDepositorInsertH2B-miRFP718nano
UseTagsmiRFP718nanoExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30-HPF1-His-Sumo-Flag
Plasmid#111577PurposeExpresses full length HPF1 in E. coliDepositorInsertHPF1 (HPF1 Human)
UseTags6xHis-Sumo-FLAGExpressionBacterialMutationPromoterT7Available sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-HTG
Plasmid#27437DepositorInserthistone-GFP-2A-TVA receptor-2A-rabies glycoprotein
UseAAV; Adeno-associated viralTagsExpressionMutationN/APromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHis-parallel1-NLSH2BCas9
Plasmid#112065PurposeExpression and purification of NLSH2BCas9 for fungal gene editingDepositorInsertNLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
UseCRISPRTagsHis tagExpressionBacterialMutationCas9 was codon optimized for Aspergillus nigerPromoterpromoter in vectorAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-H2B-AID-EYFP
Plasmid#47329Purposefor plasmid integration using the Flp-In system. AID at C-terminalDepositorInserthistone H2B (H2BC21 Human)
UseTagsAID and EYFPExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synp-F-H2B-jRGECO1a
Plasmid#102995PurposeExpresses histone fused jRGECO1a under the synapsin promoterDepositorInsertH2B-jRGECO1a
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFX-H2B-iRFP
Plasmid#175842PurposeExpress iRFP with nucleus-targeting sequence in mammalian cellsDepositorInsertiRFP with nucleus-targeting sequence
UseTagsH2B (human histone 2B)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-H2B-Venus
Plasmid#175840PurposeExpress Venus with nucleus-targeting sequence in mammalian cellsDepositorInsertVenus with nucleus-targeting sequence
UseTagsH2B (human histone 2B)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUB_smFLAG_H2B_MS2
Plasmid#81082PurposeTranslation reporter: Encodes Histone H2B (125 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).DepositorInsertsmFLAG_H2B_24xMS2
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFX-H2B-SECFP
Plasmid#175838PurposeExpress SECFP with nucleus-targeting sequence in mammalian cellsDepositorInsertSECFP with nucleus-targeting sequence
UseTagsH2B (human histone 2B)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-H2B-mCherry
Plasmid#175841PurposeExpress mCherry with nucleus-targeting sequence in mammalian cellsDepositorInsertmCherry with nucleus-targeting sequence
UseTagsH2B (human histone 2B)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-lxlplap
Plasmid#45581DepositorInsertsUseLentiviralTagsHistone 2BExpressionMammalianMutationPromoterUbiquitinAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFX-H2B-EGFP
Plasmid#175839PurposeExpress EGFP with nucleus-targeting sequence in mammalian cellsDepositorInsertEGFP with nucleus-targeting sequence
UseTagsH2B (human histone 2B)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 HDAC1
Plasmid#67101PurposeStandard for cell-free expression benchmarking.DepositorInsertHDAC1 (HDAC1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern-H2B/pcDNA3
Plasmid#51971Purposemammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to Histone H2BDepositorInsertNano-lantern
UseTagshuman H2BExpressionMammalianMutationS257G in Rluc8PromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYNL-C1_H2B
Plasmid#65702PurposeExpresses YNL-H2B in mammalian cellsDepositorInserthistone H2B
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pONL-C1_H2B
Plasmid#65704PurposeExpresses ONL-H2B in mammalian cellsDepositorInserthistone H2B
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCNL-C1_H2B
Plasmid#65703PurposeExpresses CNL-H2B in mammalian cellsDepositorInserthistone H2B
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nucGFP11-3xPax7gRNA
Plasmid#224570PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.DepositorInsertGFP11-H2B
UseCRISPRTagsHistone H2B (nuclear localization)ExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only